1887

Abstract

Rhapidosomes, tubular proteinaceous microstructures found in a variety of bacteria and algae, are resistant to disruption by many denaturing agents. They have potential application as a biomaterial and may also serve as a model for the study of self-assembly. Several reagents were tested for their ability to disrupt rhapidosomes isolated from into their component proteins. Only treatments with 01 M hydrochloric acid and 6 M guanidinium hydrochloride were found to disrupt rhapidosomes. A protocol was developed to renature the disrupted component proteins using rapid dilution and subsequent dialysis of the residual guanidinium hydrochloride. The renatured proteins were shown to have secondary structure as determined by circular dichroism. Furthermore, upon renaturation the two component proteins interacted with each other to form a complex of molecular mass > 2500 kDa. This complex, which may be a precursor in the pathway to rhapidosome formation, is currently being used to study the self-assembly process of these unique structures .

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/content/journal/micro/10.1099/13500872-141-6-1419
1995-06-01
2024-04-25
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References

  1. Avila J. 1990; Microtubule dynamics.. FASEB J 4:3284–3290
    [Google Scholar]
  2. Chang H.Y., Allen M.M. 1974; The isolation of rhapidosomes from the blue-green alga, Spirulina.. J Gen Microbiol 81:121–130
    [Google Scholar]
  3. Correll D.L., Lewin R.A. 1964; Rod shaped ribonucleoprotein particles from Saprospira.. Can J Microbiol 10:63–74
    [Google Scholar]
  4. Delk A.S., Dekker C.A. 1972; Characterization of rhapido-somes from Saprospira grandis.. J Mol Biol 64:287–295
    [Google Scholar]
  5. Evers M., Murray R. 1980; The comparison of rhapidosomes and defective bacteriophage particles from Aquaspirillum itersonii.. Can J Microbiol 26:1312–1319
    [Google Scholar]
  6. Jaenicke R., Rudolph R. 1990; Folding proteins.. in Protein Structure: a Practical Approach pp. 191–223 Creighton T.E. Edited by New York: Oxford University Press;
    [Google Scholar]
  7. Jaenicke R., Welsch R., Sara M., Sleytr U.B. 1985; Stability and self-assembly of the S-layer protein of the cell wall of Bacillus stearothermophilus.. Biol Chem Hoppe-Sejler 366:663–669
    [Google Scholar]
  8. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature 227:680–685
    [Google Scholar]
  9. Pazirandeh M., Campbell J. 1993; Protein composition of rhapidosomes isolated from Aquaspirillum itersonii.. J Gen Microbiol 139:859–864
    [Google Scholar]
  10. Pazirandeh M., Baral S., Campbell J. 1992; Metallized nanotubules derived from bacteria.. Biomimetics 1:39–48
    [Google Scholar]
  11. Sleytr U.B., Pum D., Sara M., Messner P. 1992; Twodimensional protein crystals as patterning elements in molecular nanotechnology.. In AIP Conference Proceedings 262 Molecular Electronics - Science and Technology. Aviram A. Edited by New York: American Institute of Physics;
    [Google Scholar]
  12. Takumi K., Koga T., Oka T., Endo Y. 1991; Self-assembly, adhesion, and chemical properties of tetragonally arrayed S-layer proteins of Clostridium.. J Gen Appl Microbiol 37:455–465
    [Google Scholar]
  13. Wellman S.E., Case S.T. 1989; Disassembly and reassembly in vitro of complexes of secretory proteins from Chironomus tentans salivary glands.. J Biol Chem 264:10878–10883
    [Google Scholar]
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