1887

Abstract

The effect of NaCl on growth and compatible solute utilization was investigated in a species. Growth of was observed in medium of low osmolarity (high water activity) when only 01 mM Na was present. However, lowering the water activity, by addition of KCl or sucrose, inhibited growth in this low-Na medium, but growth could be restored by the addition of NaCl. The bacterium could grow on glucose as the sole carbon source in up to 355 M NaCl and was shown also to metabolize glycine betaine. However NaCl concentrations greater than 2 M inhibited growth when glycine betaine was the sole carbon source. Glycine betaine was transported into the cells by a process stimulated by NaCl irrespective of whether the carbon source was glucose or glycine betaine. Cytoplasmic levels of glycine betaine were monitored throughout the growth cycle in 2 M NaCl medium with glycine betaine as sole carbon source. As the culture aged, glycine betaine was increasingly replaced by the tetrahydropyrimidine ectoine as the major cytoplasmic solute. The increased sensitivity to high NaCl concentrations when grown on glycine betaine may be due to the glycine betaine catabolic pathway enzymes being inhibited by the increasing external solute concentration.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-141-6-1413
1995-06-01
2019-10-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-141-6-1413
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error