1887

Abstract

SUMMARY

The uptake of nitrate or ammonium (at a concentration of 10 mol I) by marine phytoplankton was studied in relation to cell size. Initial specific nitrate uptake rates by small (35000 m) and large (130000 m) cells of the diatom did not differ significantly. However, the larger cells maintained a high uptake rate for a longer time. Therefore, they accumulated nitrate in a higher biomass-specific pool than the smaller cells. In the dark, this effect was even more pronounced. Two smaller diatom species, (7474 m) and (98 (m), had lower initial specific nitrate uptake rates and lower intracellular pools. Transient-state ammonium uptake did not result in accumulation of large intracellular pools of ammonium. Theoretically, and on the basis of the presented results, we stress the dualistic functional role of the vacuole. A large vacuole is an effective way for larger algal species to possess a minimum cell nutrient quota/cell surface ratio which is in the range of smaller species. Furthermore, by functioning as a storage reservoir it reduces inhibition of the uptake rate by cytoplasmic accumulated nutrients. The effect of the latter mechanism is that larger algal species are better at nitrate uptake under fluctuating conditions. These results imply that, in nitrogen-controlled marine systems, resource competition under fluctuating nutrient concentrations can only lead to a shift towards larger phytoplankton species if nitrate rather than ammonium is the main nitrogen source. From theoretical considerations it is argued that the maximum growth rate of algae is determined by nutrient assimilation properties rather than by photosynthetic capacity.

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1995-05-01
2021-08-05
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References

  1. Banse K. 1976; Rates of growth, respiration and photosynthesis of unicellular algae as related to cell size - a review.. J Phycol 12:135–140
    [Google Scholar]
  2. Banse K. 1982; Cell volumes, maximal growth rates of unicellular algae and ciliates, and the role of ciliates in the marine pelagial.. Limnol Oceanogr 27:1059–1071
    [Google Scholar]
  3. Blasco D., Packard T.T., Garfield P.C. 1982; Size dependence of growth rate, respiratory electron transport system activity and chemical composition of marine diatoms in the laboratory.. J Phycol 18:58–63
    [Google Scholar]
  4. Chan A.T. 1978; Comparative physiological study of marine diatoms and dinoflagellates in relation to irradiance and cell size. 1. Growth under continuous light.. J Phycol 14:396–402
    [Google Scholar]
  5. DeManche J.M., Curl H.C. Jr Lundy D.W., Donaghay P.L. 1979; The rapid response of the marine diatom Skeletonema costatum to changes in external and internal nutrient concentration.. Mar Biol 53:323–333
    [Google Scholar]
  6. Dortch Q. 1982; Effect of growth conditions on accumulation of internal nitrate, ammonium, amino acids, and protein in three marine diatoms.. J Exp Mar Biol Ecol 61:243–264
    [Google Scholar]
  7. Eppley R.W., Rogers J.N., McCarthy J.J. 1969; Half-saturation constants for uptake of nitrate and ammonium by marine phytoplankton.. Limnol Oceanogr 14:912–920
    [Google Scholar]
  8. Grasshoff K., Ehrhardt M. 1983; Automated chemical analysis.. In Methods of Seawater Analysis, 2nd edn. pp. 263–289 Grasshoff K., Ehrhardt M., Kremling K. Edited by Weinheim:: Verlag Chemie.;
    [Google Scholar]
  9. Grover J.P. 1989a; Influence of cell shape and size on algal competitive ability.. J Phycol 25:402–405
    [Google Scholar]
  10. Grover J.P. 1989b; Phosphorus-dependent growth kinetics of 11 species of freshwater algae.. Limnol Oceanogr 34:341–348
    [Google Scholar]
  11. Hobbie J.E., Daley R.J., Jasper S. 1977; Use of Nuclepore filters for counting bacteria by fluorescence microscopy.. Appl Environ Microbiol 33:1225–1228
    [Google Scholar]
  12. Laws E.A., Redalje D.G. 1979; Effect of sewage enrichment on the phytoplankton population of a subtropical estuary.. Pac Sci 33:129–144
    [Google Scholar]
  13. Margalef R. 1978; Life-forms of phytoplankton as survival alternatives in an unstable environment.. Oceanol Acta 1:493–509
    [Google Scholar]
  14. Martin J.H., Fitzwater S.E. 1988; Iron deficiency limits phytoplankton growth in the northeast Pacific subarctic.. Nature 331:341–343
    [Google Scholar]
  15. Miller A.J., Zhen R.-G. 1991; Measurement of intracellular nitrate concentrations in Chara using nitrate-selective micro-electrodes.. Planta 184:47–52
    [Google Scholar]
  16. Munk W.H., Riley G.A. 1952; Absorption of nutrients by aquatic plants.. J Mar Res 11:215–240
    [Google Scholar]
  17. Owens N.J.P., Woodward E.M.S., Aiken J., Bellan I.E., Rees A.P. 1990; Primary production and nitrogen assimilation in the North Sea during July 1987.. Neth J Sea Res 25:143–154
    [Google Scholar]
  18. Owens N.J.P., Priddle J., Whitehouse M.J. 1991; Variations in phytoplanktonic nitrogen assimilation around South Georgia and in the Bransfield Strait (Southern Ocean).. Mar Chem 35:287–304
    [Google Scholar]
  19. Parsons T.R., Takahashi M. 1973; Environmental control of phytoplankton cell size.. Limnol Oceanogr 18:511–515
    [Google Scholar]
  20. Parsons T.R., Takahashi M. 1974; A rebuttal to the comment by Hecky and Kilham.. Limnol Oceanogr 19:366–368
    [Google Scholar]
  21. Pasciak W.J., Gavis J. 1974; Transport limitation of nutrient uptake in phytoplankton.. Limnol Oceanogr 19:881–888
    [Google Scholar]
  22. Peters R.H. 1983 The Ecological Implications of Body Siɀe (Cambridge Studies in Ecology 2) Cambridge:: Cambridge University Press.;
    [Google Scholar]
  23. Price N.M., Andersen L.G., Morel F.M.M. 1991; Iron and nitrogen nutrition of equatorial Pacific plankton.. Deep-Sea Res 38:1361–1378
    [Google Scholar]
  24. Probyn T.A. 1985; Nitrogen uptake by size-fractionated phytoplankton populations in the southern Benguela upwelling system.. Mar Ecol Progr Ser 22:249–258
    [Google Scholar]
  25. Raven J.A. 1984 Energetics and Transport in Aquatic Plants (MBL Lectures in Biology 4) New York:: Alan R. Liss.;
    [Google Scholar]
  26. Raven J.A. 1986; Physiological consequences of extremely small size for autotrophic organisms in the sea.. In Photosynthetic Picoplankton. Platt T., Li W.K.W. Edited by Can Bull Fish Aquat Sci 2141–70
    [Google Scholar]
  27. Riegman R., Kuipers B.R., Noordeloos A.A.M., Witte H.J. 1993; Size differential control of phytoplankton and the structure of plankton communities.. Neth J Sea Res 31:255–265
    [Google Scholar]
  28. Sharp J.H., Perry M.J., Renger E.H., Eppley R.W. 1980; Phytoplankton rate processes in the oligotrophic waters of the central North Pacific Ocean.. J Plankton Res 2:335–353
    [Google Scholar]
  29. Shuter B.J. 1978; Size dependence of phosphorus and nitrogen subsistence quotas in unicellular microorganisms.. Limnol Oceanogr 23:1248–1255
    [Google Scholar]
  30. Smith R.E.H., Kalff J. 1982; Size-dependent phosphorus uptake kinetics and cell quota in phytoplankton.. J Phycol 18:275–284
    [Google Scholar]
  31. Sommer U. 1989; Maximal growth rates of antarctic phytoplankton : only weak dependence on cell size.. Limnol Oceanogr 34:1109–1112
    [Google Scholar]
  32. Stolte W., McCollin T., Noordeloos A.A.M., Riegman R. 1994; Effect of nitrogen source on the size distribution within marine phytoplankton populations.. J Exp Mar Biol Ecol 184:83–97
    [Google Scholar]
  33. Timmermans K.R., Stolte W., De Baar H.J.W. 1994; Iron mediated effects on nitrate reductase in marine phytoplankton.. Mar Biol 121:389–396
    [Google Scholar]
  34. Turpin D.H., Harrison P.J. 1979; Limiting nutrient patchiness and its role in phytoplankton ecology.. J Exp Mar Biol Ecol 39:151–166
    [Google Scholar]
  35. Veldhuis M.J.W., Admiraal W. 1987; The influence of phosphate depletion on the growth and colony formation of Phaeocystis pouchetii (Hariot) Lagerheim.. Mar Biol 95:47–54
    [Google Scholar]
  36. Vince S., Valiela I. 1973; The effects of ammonium and phosphate enrichments on chlorophyll a, pigment ratio and species composition of phytoplankton of Vineyard Sound.. Mar Biol 19:69–73
    [Google Scholar]
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