1887

Abstract

SUMMARY

The strictly anaerobic Gram-negative beer spoilage bacteria cerevisiae, Pectinatus cerevisiiphilus and were subjected to cellular fatty acid analysis, employing acid- and base-catalysed cleavage, gas chromatography and mass spectrometry. contained 12:0, 16:0, 16:1, 18:1, 17:cyc, 19:cyc, 12:0(3OH), 14:0(3OH) as the main fatty acids, and alk-1-enyl chains instead of acyl chains were detected to a considerable extent (14% of total fatty acids), indicating the presence of plasmalogens. The fatty acid pattern of was almost identical to that of , the only species previously assigned to this genus. and yielded fatty acids that were heavily dominated by odd-numbered chains; 11:0, 15:0, 17:1, 18:cyc and 13:0(3OH) were the main fatty acids detected in both species. Alk-1-enyl chains with similar chain lengths were also found. Both species contained six different 3-hydroxy fatty acids with chain lengths between 11 and 15 carbons, 13:0(3OH) being dominant and the others accounting for generally less than 1% of total fatty acids. Among the minor components, an unsaturated 3-hydroxy fatty acid was detected which was shown to be 13:1(30H). In addition, fatty acid analysis was shown to be applicable to detection of bacterial contamination of beer.

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1995-05-01
2024-03-29
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References

  1. Asselineau C., Asselineau J. 1984; Fatty acids and complex lipids.. In Gas Chromatography Mass Spectrometry. Applications in Microbiology pp. 57–103 Edited by Odham G., Larsson L., Mardh P.-A. New York: Plenum Press;
    [Google Scholar]
  2. Engelmann U., Weiss N. 1985; Megasphaera cerevisiae sp. nov.: a new Gram-negative obligately anaerobic coccus isolated from spoiled beer.. Syst Appl Microbiol 6:287–290
    [Google Scholar]
  3. Goldfine H. 1982; Lipids of prokaryotes - structure and distribution.. Curr Top Membr Transp 17:1–43
    [Google Scholar]
  4. Haikara A. 1991; The genera Pectinatus and Megasphaera. . In The Prokaryotes, 2nd edn.. pp. 1993–2004 Edited by Balows A., Triiper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer-V erlag;
    [Google Scholar]
  5. Helander I., Hakalehto E., Ahvenainen J., Haikara A. 1983; Characterization of lipopolysaccharides of Pectinatus cerevisiophilus. . FEMS Microbiol Lett 18:223–226
    [Google Scholar]
  6. Helander I.M., Hurme R., Haikara A., Moran A.P. 1992; Separation and characterization of two chemically distinct lipo-polysaccharides in two Pectinatus species.. J Bacteriol 174:3348–3354
    [Google Scholar]
  7. Helander I.M., Kilpeläinen I., Vaara M., Moran A.P., Lindner B., Seydel U. 1994; Chemical structure of the lipid A component of lipopolysaccharides of the genus Pectinatus. . Eur J Biochem 224:63–70
    [Google Scholar]
  8. Johnston N.C., Goldfine H. 1982; Effects of growth temperature on fatty acid and alk-l-enyl group compositions of Veillonellaparvula and Megasphaera elsdenii phospholipids.. J Bacteriol 149:567–575
    [Google Scholar]
  9. Kamio Y., Kim K.C., Takahashi H. 1971; Chemical structure of lipid A of Selenomonas ruminantium. . J Biochem 70:187–191
    [Google Scholar]
  10. Kasai N., Arata S., Mashimo J., Akiyama Y., Tanaka C., Egawa K., Tanaka S. 1987; Pseudomonas diminuta LPS with a new endotoxic lipid A structure.. Biochem Biophys Res Commun 142:972–978
    [Google Scholar]
  11. Lambert M.A., Moss C.W. 1983; Comparison of the effects of acid and base hydrolyses on hydroxy and cyclopropane fatty acids in bacteria.. J Clin Microbiol 18:1370–1377
    [Google Scholar]
  12. Lee S.Y., Mabee M.S., Jangaard N.O. 1978; Pectinatus, a new genus of the family Bacteroidaceae. . Int J Syst Bacteriol 28:582–594
    [Google Scholar]
  13. Mayberry W.R., Lane J.R. 1993; Sequential alkaline saponification/acid hydrolysis/esterification: a one-tube method with enhanced recovery of both cyclopropane and hydroxylated fatty acids.. J Microbiol Methods 18:21–32
    [Google Scholar]
  14. Moore L.V.H., Bourne D.M., Moore W.E.C. 1994; Comparative distribution and taxonomic value of cellular fatty acids in thirty-three genera of anaerobic gram-negative bacilli.. Int J Syst Bacteriol 44:338–347
    [Google Scholar]
  15. Russa R., Lüderitz O., Rietschel E.Th. 1985; Structural analyses of lipid A from lipopolysaccharides of nodulating and non- nodulating Rhispbium trifolii. . Arch Microbiol 141:284–289
    [Google Scholar]
  16. Schleifer K.H., Leuteritz M., Weiss N., Ludwig W., Kirchhof G., Seidel-Rufer H. 1990; Taxonomic study of anaerobic, Gramnegative, rod-shaped bacteria from breweries: amended description of Pectinatus cerevisiiphilus and description of Pectinatus frisingensis sp. nov., Selenomonas lacticifex sp. nov., Zymophilus raffinosivorans gen. nov., sp. nov., and Zymophilus paucivorans gen. nov., sp. nov.. Int J Syst Bacteriol 40:19–27
    [Google Scholar]
  17. Sonesson A., Jantzen E., Bryn K., Larsson L., Eng J. 1989; Chemical composition of a lipopolysaccharide from Eegionella pneumophila. . Arch Microbiol 153:72–78
    [Google Scholar]
  18. Urbanik-Sypniewska T., Seydel U., Greck M., Weckesser J., Mayer H. 1989; Chemical studies on the lipopolysaccharide of Rhi^obium meliloti 10406 and its lipid A region.. Arch Microbiol 152:527–532
    [Google Scholar]
  19. Sonesson A., Jantzen E., Tangen T., Zähringer U. 1994; Lipopolysaccharides of Eegionella erythra and Eegionella oakridgensis. . Can J Microbiol 40:666–671
    [Google Scholar]
  20. Van Golde L. M. G., Prins R. A., Franklin-Klein W., Akkermans- Kruyswijk J. 1973; Phosphatidylserine and its plasmalogen analogue as major lipid constituents in Megasphaera elsdenii. . Biochim Biophys Acta 326:314–323
    [Google Scholar]
  21. Vulliet P., Markey S.P., Tornabene T.G. 1974; Identification of methoxyester artifacts produced by methanolic-HCl solvolysis of the cyclopropane fatty acids of the genus Yersinia. . Biochim Biophys Acta 348:299–301
    [Google Scholar]
  22. Wackerbauer K., Bender G. 1983; Die Bedeutung der freien Fettsäuren und Fettsäure-Äthylester wahrend der Gärung und Lagerung.. Monatssch Brauwiss 36:152–158
    [Google Scholar]
  23. Wilkinson S.G. 1988; Gram-negative bacteria.. In Microbial Lipids 1: pp. 299–488 Edited by Ratledge C., Wilkinson S. G. London: Academic Press;
    [Google Scholar]
  24. Wollenweber H.-W., Rietschel E.T. 1990; Analysis of lipo- polysaccharide (lipid A) fatty acids.. J Microbiol Methods 11:195–211
    [Google Scholar]
  25. Zähringer U., Lindner B., Rietschel E.T. 1994; Molecular structure of lipid A, the endotoxic center of bacterial lipo- polysaccharides.. Adv Carbohydr Chem Biochem 50:211–276
    [Google Scholar]
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