1887

Abstract

SUMMARY

The enzyme glutamine synthetase (GS) is described for the first time in The appearance of this enzyme is developmentally regulated. The level of activity is low in vegetative cells and increases more than threefold during differentiation. Furthermore this enzyme is shown to be differentially localized in prespore cells, the specific activity being approximately fourfold higher than in prestalk cells. The enzyme has a pH optimum of 7.8 and 8.2 in the γ-glutamyltransferase and γ-glutamylsynthetase assays, respectively, and a temperature optimum of 45°C. Kinetic studies of GS revealed apparent K values of 5.9 mM, 0.009 mM and 8.6 mM for glutamine, ADP and NHOH, respectively, in the γ-glutamyltransferase assay, and of 2.2 mM, 0.12 mM and 0.64 mM for glutamate, ATP and NHOH, respectively, in the γ-glutamylsynthetase assay.

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1995-05-01
2021-05-18
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