1887

Abstract

SUMMARY

The products of anaerobic metabolism of glucose and its derivatives sorbitol, gluconate and glucuronate by have been determined by proton NMR. Glucose was fermented through mixed-acid fermentation pathways to acetate, 2,3-butanediol, ethanol, formate, lactate, succinate and pyruvate. However, the bacterium was incapable of fermenting the three glucose derivatives. When cells were incubated anaerobically with glucose in the presence of nitrate, the reduced products and formate did not appear and acetate was formed as the major metabolite. Growth and formation of acetate was also observed when cells were incubated anaerobically with each of the three glucose derivatives, in the presence of nitrate. A formate-nitrate oxido-reductase system was induced under anaerobic conditions, with increased activities when nitrate was added to the anaerobic growth medium. However no activity was detected when cell; were grown in the presence of molecular oxygen. Formate-nitrate oxido-reductase activity was absent in chlorate-resistant mutants isolated spontaneously or following Tn insertional mutagenesis. The spontaneous mutants fermented glucose in the presence of nitrate suggesting that they were incapable of nitrate respiration, due to a deficiency in one or more components of the formate-nitrate oxido-reductase system. Two insertional mutants exhibited elevated -galactosidase activity when grown in the presence of nitrate.

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1995-05-01
2021-04-18
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References

  1. Alam K.Y., Clark D.P. 1989; Anaerobic fermentation balance of Escherichia coli as observed by in vivo NMR spectroscopy.. J Bacteriol 171:6213–6217
    [Google Scholar]
  2. Anagnostopoulos C., Spizizen J. 1961; Requirements for transformation in Bacillus subtilis. . J Bacteriol 81:741–746
    [Google Scholar]
  3. Blasco F., Iobbi C., Giordano G., Chippaux M., Bonnefy V. 1989; Nitrate reductase of Escherichia coli: completion of the nucleotide sequence of the nar operon and reassessment of the role of the alpha and beta subunits in iron binding and electron transfer.. Mol & Gen Genet 218:249–256
    [Google Scholar]
  4. Choe M., Reznikoff W.S. 1991; Anaerobically expressed Escherichia coli genes identified by operon fusion techniques.. J Bacteriol 173:6139–6146
    [Google Scholar]
  5. Clark D.P. 1989; The fermentation pathways of Escherichia coli. . FEMS Microbiol Rev 63:223–234
    [Google Scholar]
  6. Cole J.A. 1978; The rapid accumulation of large quantities of ammonia during nitrite reduction by Escherichia coli. . FEMS Microbiol Lett 4:327–329
    [Google Scholar]
  7. Cole J.A. 1982; Independent pathways for the anaerobic reduction of nitrite to ammonia in Escherichia coli. . Biochem Soc Trans 10:476–478
    [Google Scholar]
  8. Cole i.A., Brown C.M. 1980; Nitrite reduction to ammonia by fermentative bacteria: a short circuit in the biological nitrogen cycle.. FEMS Microbiol Lett 7:65–72
    [Google Scholar]
  9. Downey R.J., Kiszkiss D.F. 1969; Oxygen and nitrate induced modification of the electron transfer system of Bacillus stearothermophilus. . Microbios 2:145–153
    [Google Scholar]
  10. Downey R.J., Kiszkiss D.F., Nuner J.H. 1969; Influence of oxygen on development of nitrate respiration in Bacillus stearothermophilus. . J Bacteriol 98:1056–1062
    [Google Scholar]
  11. Garzon A., Li J., Flores A., Casadesus J., Stewart V. 1992; Molybdenum cofactor (chlorate resistant) mutants of Klebsiella pneumoniae M5al can use hypoxanthine as the sole nitrogen source.. J Bacteriol 74:6298–6302
    [Google Scholar]
  12. Glaser J.H., De Moss J.A. 1972; Comparison of nitrate reductase mutants of Escherichia coli selected by alternative procedures.. Mol & Gen Genet 116:1–10
    [Google Scholar]
  13. Gunsalus R.P. 1992; Control of electron flow in Escherichia coli: coordinated transcription of respiratory pathway genes.. J Bacteriol 174:7069–7074
    [Google Scholar]
  14. Johann S., Hinton S.M. 1987; Cloning and nucleotide sequence of the chlD locus.. J Bacteriol 169:1911–1916
    [Google Scholar]
  15. Pichinoty F., Garda J.-L., Durand C.J. 1977; La de’nitrification chez Bacillus licheniformis. . Can J Microbiol 24:45–49
    [Google Scholar]
  16. Priest F.G. 1977; Extracellular enzyme synthesis in the genus Bacillus. . Bacteriol Rev 41:711–753
    [Google Scholar]
  17. Priest F.G. 1993; Systematics and ecology of Bacillus. . In Bacillus subtilis and Other Gram-positive Bacteria pp. 3–16 Sonenshein A.L., Hoch J., Losick R. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  18. Rajagopalan K.V., Johnson J.L. 1992; The pterin molybdenum cofactors.. J Biol Chem 267:10199–10202
    [Google Scholar]
  19. Raspoet D., Pot B., De Deyn D., Devos P., Kersters K., Deley J. 1991; Differentiation between 2,3-butanediol producing Bacillus licheniformis and B. polymyxa strains by fermentation product profiles and whole-cell protein electrophoretic patterns.. Syst Appl Microbiol 14:1–7
    [Google Scholar]
  20. Sawers G., Bock A. 1988; Anaerobic regulation of pyruvate formate-lyase from Escherichia coli K-12.. J Bacteriol 170:5330–5336
    [Google Scholar]
  21. Schulp J.A., Stouthamer A.H. 1972; Isolation and characterization of mutants resistant against chlorate of Bacillus licheniformis. . J Gen Microbiol 73:95–112
    [Google Scholar]
  22. Spiro S., Guest J.R. 1991; Adaptive responses to oxygen limitation in Escherichia coli. . Trends Biochem Sci 16:310–314
    [Google Scholar]
  23. Stewart V. 1982; Requirement of fnr and narL functions for nitrate reductase expression in Escherichia coli K-12.. J Bacteriol 151:1320–1325
    [Google Scholar]
  24. Tangney M., Priest F.G., Mitchell W.J. 1993; Two glucose transport systems in Bacillus licheniformis. . J Bacteriol 175:2137–2142
    [Google Scholar]
  25. Van Leen R.W., Bakhuis J.G., Van Beckhoven R.F.W.C., Burger H., Dorssers L.C.J., Hommes R.W.J., Lemson P.J., Noordam B., Persoon N.L.M., Wagemaker G. 1991; Pro- duction of human interleukin-3 using industrial microorganisms.. Biol Technology 9:47–52
    [Google Scholar]
  26. Van ’Triet J., Wientjes F.B., Van Doom J., Planta R.J. 1979; Purification and characterization of the respiratory nitrate reductase of Bacillus licheniformis. . Biochim Biophys Acta 576:347–360
    [Google Scholar]
  27. Wati M.R., Priest F.G., Mitchell W.J. 1990; Mutagenesis using Tn917 in Bacillus licheniformis. . FEMS Microbiol Lett 71:211–214
    [Google Scholar]
  28. Youngman P. 1990; Use of transposons and integrational vectors for mutagenesis and construction of gene fusions in Bacillus. . In Molecular Biological Methods for Bacillus pp. 221–226 Harwood C.R., Cutting S.M. Edited by Chichester: Wiley;
    [Google Scholar]
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