1887

Abstract

A toxin gene () on a 2·9 kb DNA fragment of B-4ac was cloned and expressed in , and its nucleotide sequence determined. The DNA fragment contained an open reading frame capable of encoding a polypeptide of 336 amino acids with a molecular mass of 41039 Da. The translated product in exhibited Vero cell cytotoxicity, and was positive in a vascular permeability assay. It also caused fluid accumulation in a ligated mouse ileal loop and was lethal to mice upon injection. These biological activities are considered characteristic of diarrhoeal enterotoxins. We therefore conclude that this gene, designated , encodes one of the enterotoxic proteins of which cause food-borne diarrhoea.

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1995-04-01
2021-05-09
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