1887

Abstract

is a biocontrol agent that attacks a range of economically important phytopathogenic fungi. In an attempt to identify genes specifically expressed by T. harzianum during growth on cell walls of , we carried out differential screening of an induced cDNA library. In this paper we report the analysis of the sequence and expression of two cDNA clones that encode putative mycoparasitism-related proteins of T. harzianum. One of these clones corresponds to a gene, inda 1, that encodes a protein of 570 amino acids with a predicted molecular mass of 62853 Da. The predictedamino acid sequence of inda1 showed a high degree of similarity with amino acid permeases from several other organisms. The other cDNA clone corresponds to a gene, indc11, that encodes a novel protein of 340 ami acids with a predicted molecular mass of 37010 Da. The use of this methodology should provide specific genetic markers to follow mycoparasitism by Trichoderma spp.

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1995-04-01
2021-07-28
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