1887

Abstract

SMF13 produced leupeptin, chymotrypsin-like protease (CTP), metalloprotease, and trypsin-like protease (TLP) extracellularly. The activity of TLP was specifically inhibited by leupeptin. Production of leupeptin was closely associated with growth but leupeptin was inactivated by leupeptin-inactivating protein (LIP) when growth reached the stationary phase in submerged cultures, or when aerial mycelia started to form on surface cultures. Autolysis of mycelia after the stationary phase in submerged cultures was apparently retarded by the addition of leupeptin; on surface cultures, aerial mycelium formation was clearly retarded by the addition of leupeptin. We propose that CTP participates primarily in utilization of a proteinaceous nitrogen source, that TLP functions as an essential enzyme involved in the metabolism of mycelial protein, that leupeptin inhibits the activity of TLP and that LIP inactivates leupeptin. The cascade of regulatory actions of the compounds, which are produced sequentially during mycelium development, may provide selective advantages in adverse culture conditions.

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1995-04-01
2021-10-16
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References

  1. Aoyagi T. 1989; Protease inhibitor and biological control. In Bioactive Metabolites from Microorganisms: Progress in Industrial Microbiology, vol. 27 pp. 403–418 Edited by Bussel M.E., Grafe U. New York: Elsevier;
    [Google Scholar]
  2. Aoyagi T., Miyata S., Nanbo M., Kojima F., Matsuzaki M., Ishisuka M., Takeuchi T., Umezawa H. 1969; Biological activities of leupeptins. J Antibiot 22:558–568
    [Google Scholar]
  3. Aoyagi T., Ishizuka M., Takeuchi T., Umezawa H. 1977; Enzyme inhibitors in relation to cancer therapy. J Antibiot 30:121–132
    [Google Scholar]
  4. Bond J. S., Butler P. E. 1987; Intracellular proteases.. Annu Rev Biochem 56:333–364
    [Google Scholar]
  5. Bradley S. G. 1988; Actinomycete protease and their role in regulation. In Biology of Actinomycetes-’88 pp. 433–438 Edited by Okami Y., Beppu T., Ogawara H. Tokyo: Japan Scientific Societies Press;
    [Google Scholar]
  6. Chater K. F. 1984; Morphological and physiological differentiation in Streptomyces . In Microbial Development pp. 89–115 Edited by Losik R., shapiro L. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  7. Chater K. F., Merrick M. J. 1979; Streptomyces. In Developmental Biology of Prokaryotes, pp. 93–114 Edited by Parish J. H. Oxford: Blackwells;
    [Google Scholar]
  8. Gibb G. D., Strohl W. R. 1988; Physiological regulation of protease activity in Streptomyces peucetius . Can J Microbiol 34:187–190
    [Google Scholar]
  9. Ginther C. L. 1978; Sporulation and the production of serine protease and cephamycin C by Streptomyces lactamdurans . Antimicrob Agents Chemother 15:522–526
    [Google Scholar]
  10. Gräfe U. 1989; Autoregulatory secondary metabolite from actinomycetes.. In Regulation of Secondary Metabolism in Actinomycetes pp. 75–126 Edited by Shapiro S. Boca Raton, FL: CRC Press;
    [Google Scholar]
  11. Holzer H. 1983; Regulation of proteinase in Saccharomyces cerevisiae . In Protease Inhibitors: Medical and Biological Aspects, pp. 181–190 Edited by Katunuma N., Umezawa H., Holzer H. Tokyo: Japan Scientific Society Press;
    [Google Scholar]
  12. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H., Lydiate D. J., Smith C. P., Ward J., Schrempf H. 1985 Genetic Manipulation of Streptomyces: a Laboratory Manual Norwich: John Innes Foundation;
    [Google Scholar]
  13. Kim I. S., Kim H. T., Ward A. C., Goodfellow M., Hah Y.C, Lee K. J. 1992; Numerical identification of a streptomyces strain producing thiol protease inhibitor. J microbiol biotechnol 2220–225
    [Google Scholar]
  14. Kim I. S., Han Y. T., Barrow K. D., Lee K. J. 1993; The structure of protease inhibitors produced by Streptomyces exfoliatus SMF13. Korean J Microbiol 31:326–334
    [Google Scholar]
  15. Mendez C., Braña A. F., Manzanal M. B., Hardisson C. 1985; Role of substrate mycelium in colony development in Streptomyces . Can J Microbiol 31:446–450
    [Google Scholar]
  16. Miller G. L. 1959; Use of dinitrosalicilic acid reagent for determination of reducing sugar. Anal Chem 31:426–428
    [Google Scholar]
  17. Narahashi Y. 1970; Pronase. Methods Ensymol 19:651–664
    [Google Scholar]
  18. Pirt S. J. 1975; Parameters of growth and analysis of growth data.. In Principles of Microbe and Cell Cultivation pp. 4–14 Oxford: Blackwells;
    [Google Scholar]
  19. Sarath G., De La Motte R. S., Wagner F. W. 1989; Protease assay methods.. In Proteolytic Enzymes: a Practical Approach pp. 25–55 Edited by Beynone R. J., Bond J. S. Oxford: IRL Press;
    [Google Scholar]
  20. Shapiro S. 1989; Nitrogen assimilation in actinomycetes and the influence of nitrogen nutrition on actinomycete secondary metabolism.. In Regulation of Secondary Metabolism in Actinomycetes pp. 149–153 Edited by Shapiro S. Boca Raton FL: CRC Press;
    [Google Scholar]
  21. Suda H., Aoyagi T., Hamada M., Takeuchi T., Umezawa H. 1972; Antipain, a new protease inhibitor isolated from actinomycetes. J Antibiot 25:263–266
    [Google Scholar]
  22. Umezawa H. 1982; Low-molecular-weight enzyme inhibitors from microbial origin. Annu rev Microbiol 36:75–99
    [Google Scholar]
  23. Umezawa H. 1988; Low-molecular-weight enzyme inhibitors and immunomodifiers.. In Actinomycetes in Biotechnology pp. 285–325 Edited by Goodfellow M., Williams S. T., Mordarski M. London: Academic Press;
    [Google Scholar]
  24. Umezawa H., Aoyagi T., Morishima H., Kunimoto S., Mat- suzaki M., Hamada M., Takeuchi T. 1970; Chymostatin, a new chymotrypsin inhibitor produced by actinomycetes. J Antibiot 23:425–427
    [Google Scholar]
  25. Umezawa H., Aoyagi T., Okura A., Morishima H., Takeuchi T., Okami Y. 1973; Elastatinal, a new elastase inhibitor produced by actinomycetes. J Antibiot 26:787–789
    [Google Scholar]
  26. Wildermath H. 1970; Development and organization of the aerial mycelium in Streptomyces coelicolor.. J Gen Microbiol 60:43–50
    [Google Scholar]
  27. Yuan L., Cole G. T. 1989; Characterization of a proteinase inhibitor isolated from the fungal pathogen Coccidioides immitis.. Biochem J 257:729–736
    [Google Scholar]
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