RT Journal Article SR Electronic(1) A1 Filippini, Silvia A1 Solinas, Maria Manuela A1 Breme, Umberto A1 Schlüter, Manuela Birgit A1 Gabellini, Davide A1 Biamonti, Giuseppe A1 Colombo, Anna Luisa A1 Garofano, LuisaYR 1995 T1 Streptomyces peucetius daunorubicin biosynthesis gene, dnrF: sequence and heterologous expression JF Microbiology, VO 141 IS 4 SP 1007 OP 1016 DO https://doi.org/10.1099/13500872-141-4-1007 PB Microbiology Society, SN 1465-2080, AB The dnrF gene, responsible for conversion of aklavinone to ɛ-rhodomycinone via C-11 hydroxylation, was mapped in the daunorubicin (Dnr) gene cluster of Streptomyces peucetius ATCC 29050, close to drrAB, one of the anthracycline-resistance genes. The dnrF gene was sequenced and should encode a protein of 489 amino acids with a molecular mass of 52 kDa. The deduced DnrF protein shows significant similarities with bacterial FAD- and NADPH-dependent hydroxylases either required to introduce hydroxyl groups into polycyclic aromatic polyketide antibiotics or involved in catabolism of aromatic compounds. Heterologous expression of dnrF in Streptomyces lividans TK23 and in Escherichia coli demonstrated that the gene encodes a NADPH-dependent hydroxylase catalysing the hydroxylation of aklavinone to yield ɛ-rhodomycinone. The enzyme is inactive on anthracyclines glycosylated at position C-7 and its activity decreases to a different extent with other substrate modifications, indicating that DnrF has a significant substrate specificity., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-141-4-1007