The location and organization of repetitive sequences, members of the RPS family, which are sequences specific to Candida albicans, were determined on each chromosome of C. albicans strain FC18. Using pulsed-field gel electrophoresis, we separated seven fractions from eight chromosomes. Each chromosome was cleaved by BamHI and XhoI to excise the RPSs, which were then detected by hybridization with an RPS probe. All chromosomes except chromosome 4 carried RPSs, and these RPSs were located within a limited region on each chromosome. From the digestion of each chromosome with Sfil and probing with the RPSs, we found that these recognition sites within the RPS region were conserved among all RPS-containing chromosomes. For further characterization of the RPSs, the locations and the boundary regions of the RPSs were examined on chromosome 6 of strain FC18 as a model chromosome. Using the restriction enzymes Sfil, Smal, Xhol, BamHl, Mlul and Nrul, we constructed a semi-macro physical map of the RPSs and their boundary regions on this chromosome. We also determined which part of the RPS was adjacent to each boundary by using sub-fragments of RPS as probes. The physical configuration around the RPSs and their boundary regions are presented. The results obtained should be useful for future analysis of the function of these regions.
ChibanaH.,
IwaguchiS.-I.,
HommaM.,
ChindampornA.,
NakagawaY.,
TanakaK.1994; Diversity of tandemly repetitive sequences due to periodic repetitions in the chromosomes of Candida albicans
. J Bacteriol 176:3851–3858
ChindampornA.,
IwaguchiS.-I.,
NakagawaY.,
HommaM.,
TanakaK.1993; Clonal size-variation of rDNA cluster region on chromosome XII of Saccharomyces cerevisiae
. J Gen Microbiol 139: 1409–1415
DoiM.,
HommaM.,
ChindampornA.,
TanakaK.1992; Estimation of chromosome number and size by pulsed-field gel electrophoresis (PFGE) in medically important Candida species. J Gen Microbiol 138: 2243–2251
IwaguchiS.-I.,
HommaM.,
TanakaK.1990; Variation in the electrophoretic karyotype analysed by the assignment of DNA probes in Candida albicans
. J Gen Microbiol 136: 2433–2442
IwaguchiS.-I.,
HommaM.,
ChibanaH.,
TanakaK.(1992a); Isolation and characterization of a repeated sequence (RPS1) of Candida albicans
. J Gen Microbiol 138: 1893–1900
IwaguchiS.-I.,
HommaM.,
TanakaK.1992b; Clonal variation of chromosome size derived from the rDNA cluster region in Candida albicans
. J Gen Microbiol 138: 1177–1184
KurtzM. B.,
CortelyouM. W.,
MillerS. M.,
KirschD. R.1987; Development of autonomously replicating plasmids for Candida albicans
. Mol Cell Biol 7: 209–217
LaskerB. A.,
CarleG. F.,
KobayashiG. S.,
MedoffG.1989; Comparison of the separation of Candida albicans chromosome-sized DNA by pulsed-field gel electrophoresis techniques. Nucleic Acids Res 17: 3783–3793
LottT. J.,
BoironP.,
ReissE.1987; An electrophoretic karyotype for Candida albicans reveals large chromosomes in multiples. Mol & Gen Genet 209: 170–174
Rustchenko-BulgacE. P.,
ShermanF.,
HicksJ. B.1990; Chromosomal rearrangements associated with morphological mutants provide a means for genetic variation of Candida albicans
. J Bacteriol 172:1276–1283
SadhuC.,
McEachernM. J.,
Rustchenko-BulgacE. P.,
SchmidJ.,
SollD. R.,
HicksJ. B.1991; Telomeric and dispersed repeat sequences in Candida yeasts and their use in strain identification. J Bacteriol 173:842–850
SuzukiT.,
KobayashiI.,
KanbeT.,
TanakaK.1989; High- frequency variation of colony morphology and chromosome reorganization in the pathogenic yeast Candida albicans
. J Gen Microbiol 135:425–434
TakahashiK.,
MurakamiS.,
ChikashigeY.,
FunabikiH.,
NiwaO.,
YanagidaM.1992; A low copy number central sequence with strict symmetry and unusual chromatin structure in fission yeast centromere. Mol Biol Cell 3:819–835