SUMMARY: The effect of glucose on a number of mitochondrial and cytoplasmic enzymes involved in carbon metabolism (L-lactate:ferricytochrome-c 2-oxidoreductase, malate dehydrogenase, β-galactosidase, invertase, maltase and NAD-glutamate dehydrogenase) has been analysed in two different strains of (PM4-4B and JA6 strains). All the above mentioned enzymes were catabolite-repressible in a strain-dependent way. From this study differences in the regulation of some enzymes have been observed between and To identify genes involved in glucose metabolism that may also control carbon catabolite repression, 2-deoxyglucose-resistant mutants (Dgr) of that were unable to grow on glucose in the presence of antimycin A (Rag) were selected. In this way we identified four classes of mutants. Two of them define genes previously identified: and , encoding the low affinity glucose transport system and hexokinase, respectively. The two remaining classes of mutants define two new genes, and , that control the level of hexokinase, indicating that in this enzyme is positively regulated by at least two genes. All the mutants devoid of hexokinase showed relief from carbon catabolite repression of several enzymes.


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