RT Journal Article SR Electronic(1) A1 Foglino, M. A1 Borne, F. A1 Bally, M. A1 Ball, G. A1 Patte, J. C.YR 1995 T1 A direct sulfhydrylation pathway is used for methionine biosynthesis in Pseudomonas aeruginosa JF Microbiology, VO 141 IS 2 SP 431 OP 439 DO https://doi.org/10.1099/13500872-141-2-431 PB Microbiology Society, SN 1465-2080, AB SUMMARY: The relationship between genes and enzymes in the methionine biosynthetic pathway has been studied in Pseudomonas aeruginosa. The first step is catalysed by an O-succinylhomoserine synthase, the product of the metA gene mapped at 20 min on the chromosome. The second step is achieved by direct sulfhydrylation, involving the enzyme encoded by a metZ gene that we have identified and sequenced, located at 40 min. Thus Pseudomonas appears to be the only organism so far described that uses O-succinylhomoserine as substrate for a direct sulfhydrylation. As in yeast, the two transsulfuration pathways between cysteine and homocysteine, with cystathionine as an intermediate, probably exist in parallel in this organism., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-141-2-431