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The 29·71 kb chromosomal region of Bacillus subtilis 168 extending from 308° to 311° contains 18 ORFs. Functions of most of these ORFs were identified and associated with cell wall metabolism. Sequences of two non-coding regions of 0·7 and 2·2 kb flanking the ggaAB operon involved in the synthesis of poly(3-O-β-D-glucopyranosyl N-acetylgalactosamine 1-phosphate), a minor teichoic acid, correspond to five degenerate segments of neighbouring protein-coding regions. We discuss the possibility that such grey holes are indicative of a chromosomal rearrangement which could have arisen from horizontal gene transfer.
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