SUMMARY: The gene encoding thymidylate synthase A was cloned from a genomic library of 168. The sequence of was found to be highly similar to that of the phage ø3T thymidylate synthase gene. This similarity is, however, limited to about 862 nucleotides, spanning the coding region and the adjacent 3” region. The flanking sequences are not related. An integrative plasmid containing a DNA fragment with a deletion within the coding region of was constructed and used to replace the chromosomal gene. Transformants unable to grow without thymidine at 47 °C were obtained. Genetic and physical mapping techniques were used to show that the cloned DNA fragment harbouring δ was integrated at 168° on the chromosome.


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