SUMMARY: The gene encoding extracellular xylanase () was amplified as a 770 bp DNA fragment from 168 chromosomal DNA by PCR. The genes encoding endo-β-1,4-glucanase () and endo-β-1,3-1,4-glucanase () were isolated from a genomic library of 168. The sequences of and were identical to those of the xylanase and cellulase genes from PAP115. Integrative plasmids containing DNA fragments with deletions in the coding region of the genes were constructed and used to replace the chromosomal and genes of 168. Strains without any detectable activity against xylan (Xyn-), carboxymethylcellulose (Egl-) or mixed linked β-1,3-1,4-glucan (Egl- Bgl-) were obtained. The genes were mapped at 170° 175° and 340° on thechromosome.


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