1887

Abstract

SUMMARY:

A 21 548 bp nucleotide sequence around the 24° region of the chromosome, located 306 kb downstream from the zero point of the physical map, was determined. Twenty-one putative ORFs were identified: two ORFs (Orf1 and Orf20) were consistent with the nucleotide and amino acid sequence of OrfJ, whose function is not known, and Pcp, respectively; four were found to display significant similarities to known proteins in data banks, i.e. 5-keto-3-deoxyglucarate dehydratase (Orf2), aldehyde dehydrogenase (Orf3) and two glucarate dehydratases (Orf4 and 5); three had considerable similarity to the sensor-regulator proteins of bacterial two-component signal transduction systems (Orf1, 11 and 12); two had considerably high levels of similarity to parts of known proteins (Orf13 and 19); and five showed low levels of similarity to known proteins in the data banks. The remaining six ORFs showed no similarity to known proteins.

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1995-02-01
2021-10-21
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References

  1. Arthur M., Molinas C., Courvalin P. 1992; The VanS-VanR two-component regulatory system controls synthesis of depsi- peptide peptidoglycan precursors in Enterococcus faecium BM4147. J Bacteriol 174:2582–2591
    [Google Scholar]
  2. Awade A., Cleuziat P., Gonzales T., Robert-Baudouy J. 1992; Characterization of the pcp gene encoding the pyrrolidone carboxyl peptidase of Bacillus subtilis . FEBS Lett 305:67–73
    [Google Scholar]
  3. Bibb M. J., Bibb Ma. J., Ward J. M., Cohen S. N. 1985; Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes indigenous to Streptomyces . Mol & Gen Genet 199:26–36
    [Google Scholar]
  4. Comeau D. E., Ikenaka K., Tsung K., Inouye M. 1985; Primary characterization of the protein products of the Escherichia coli ompB locus: structure and regulation of synthesis of the OmpR and EnvZ proteins. J Bacteriol 164:578–584.
    [Google Scholar]
  5. Daniels D.L, III Plunkett G., Burland V., Blattner F. R. 1992; Analysis of the Escherichia coli genome: DNA sequence of the region from 84·5 to 86·5 minutes. Science 257:771–778
    [Google Scholar]
  6. Drummond M., Whitty P., Wootton J. 1986; Sequence and domain relationships of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins. EMBO J 5:441–447
    [Google Scholar]
  7. Guest J. R. 1987; Functional implications of structural homologies between chloramphenicol acetyltransferase and dihydro- lipoamide acetyltransferase. FEMS Microbiol Lett 44:417–422
    [Google Scholar]
  8. Hempel J., Bahr-Lindstrom H., Jornvell H. 1984; Aldehyde dehydrogenase from human liver. Primary structure of the cytoplasmic isoenzyme. Eur J B¿ochem 141:21–35.
    [Google Scholar]
  9. Hidalgo E., Chen Y. M., Lin E., Aguilar J. 1991; Molecular cloning and DNA sequencing of the Escherichia coli K-12 ald gene encoding aldehyde dehydrogenase. J Bacteriol 1736118–6123
    [Google Scholar]
  10. Hoshino T., Kose K. 1990; Cloning, nucleotide sequences, and identification of products of the Pseudomonas aeruginosa PAO bra genes, which encode the high-affinity branched-chain amino acid transport system. J Bacteriol 172:5531–5539
    [Google Scholar]
  11. Imanaka T., Ohta T., Sakoda H., Widhyastuti N., Matsuoka M. 1993; Cloning, nucleotide sequence and efficient expression of the gene coding for thermostable aldehyde dehydrogenase from Bacillus stearothermophilus, and characterization of the enzyme. J Ferment Bioeng 76:161–167
    [Google Scholar]
  12. Itaya M., Tanaka T. 1991; Complete physical map of the Bacillus subtilis 168 chromosome constructed by a gene-directed mutagenesis method. J Mol Biol 220:631–648
    [Google Scholar]
  13. Johann S., Hinton S. M. 1987; Cloning and nucleotide sequence of the chlD locus. J Bacteriol 169:1911–1916
    [Google Scholar]
  14. Kessler U., Maid U., Zetsche K. 1992; An equivalent to bacterial omp'R genes is encoded on the plastid genome of red algae. Plant Mol Biol 18777–780
    [Google Scholar]
  15. Lamark T., Kaasen I., Eshoo M. W., Falkenberg P., McDougall J., Strom A. R. 1991; DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia colt . Mol Microbiol 5:1049–1064
    [Google Scholar]
  16. Makino K., Shinagawa H., Amemura M., Nakata T. 1986; Nucleotide sequence of the phoB gene, the positive regulatory gene for the phosphate regulon of Escherichia coli K-12. J Mol Biol 190:37–44
    [Google Scholar]
  17. Miranda-Rios J., Sanchez-Pescador R., Urdea M., Cavarrubias A. A. 1987; The complete nucleotide sequence of the gln ALG operon of Escherichia coli K12. Nucleic Acids Res 15:2757–2770.
    [Google Scholar]
  18. Munro A. W., Ritchie G. Y., Lamb A. J., Douglas R. M., Booth I. R. 1991; The cloning and DNA sequence of the gene for the glutathione-regulated potassium-efflux system KefC of Escherichia coli . Mol Microbiol 5:607–616
    [Google Scholar]
  19. Nagasawa S., Tokishita S., Aiba H., Mizuno T. 1992; A novel sensor-regulator protein that belongs to the homologous family of signal-transduction proteins involved in adaptive responses in Escherichia coli . Mol Microbiol 6:799–807
    [Google Scholar]
  20. Russo F. D., Slauch J. M., Silhavy T. J. 1993; Mutations that affect separate functions of OmpR, the phosphorylated regulator of porin transcription in Escherichia coli . J Mol Biol 231:261–271
    [Google Scholar]
  21. Saito H., Miura K. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Bìophys Acta 72:619–629
    [Google Scholar]
  22. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Seki T., Yoshikawa H., Takahashi H., Saito H. 1987; Cloning and nucleotide sequence of phoP, the regulatory gene for alkaline phosphatase and phosphodiesterase in Bacillus subtilis . J Bacteriol 169:2913–2916
    [Google Scholar]
  24. Seki T., Yoshikawa H., Takahashi H., Saito H. 1988; Nucleotide sequence of the Bacillus subtilis phoR gene. J Bacteriol 170:5935–5938
    [Google Scholar]
  25. Starnbach M. N., Lory S. 1992; The fliA (rpoF) gene of Pseudomonas aeruginosa encodes an alternative sigma factor required for flagellin synthesis. Mol Microbiol 6:459–469
    [Google Scholar]
  26. Sun D., Setlow P. L. 1993; Cloning, nucleotide sequence and regulation of the Bacillus subtilis nadB gene and a nifS-like gene both of which are essential for NAD biosynthesis. J Bacteriol 175:1423–1432
    [Google Scholar]
  27. Walderhaug M. O., Daniel J. M., Hesse J., Epstein W. 1992; KdpD and KdpE, regulatory proteins for the kdp operon of Escherichia coli, are members of the two component sensor-effector class. J Bacteriol 174:2152–2159
    [Google Scholar]
  28. Weretilnyk E. A., Hanson A. D. 1990; Molecular cloning of a plant betaine-aldehyde dehydrogenase, an enzyme implicated in adaptation to salinity and drought. Proc Natl Acad Set USA 87:2745–2749
    [Google Scholar]
  29. Wright G. D., Holman T. R., Walsh G T. 1993; Purification and characterization of VanR and the cytosolic domain of VanS: a two-component regulatory system required for vancomycin resistance in Enterococcus faecìum BM4147. Biochemistry 32:5057–5063
    [Google Scholar]
  30. Yamazaki H., Ohmura K., Nakayama A., Takeichi Y., Otozai K., Yamazaki M., Tamura G., Yamane K. 1983; α-Amylase genes (amyR2 and amyE) from an α-amylase hyperproducing Bacillus subtilis: molecular cloning and nucleotide sequence. J Bacteriol 156:327–337
    [Google Scholar]
  31. Yang M., Galizzi A., Henner D. 1983; Nucleotide sequence of the amylase gene from Bacillus subtilis . Nucleic Acids Res 11:237–249
    [Google Scholar]
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