@article{mbs:/content/journal/micro/10.1099/13500872-141-2-261, author = "Moszer, Ivan and Glaser, Philippe and Danchin, Antoine", title = "Subtilist: a relational database for the Bacillus subtilis genome", journal= "Microbiology", year = "1995", volume = "141", number = "2", pages = "261-268", doi = "https://doi.org/10.1099/13500872-141-2-261", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-141-2-261", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "Bacillus subtilis", keywords = "codon usage", keywords = "database", keywords = "genome sequencing", keywords = "isoelectric point", abstract = "SUMMARY In the framework of the international collaborative project aiming to sequence the whole Bacillus subtilis chromosome, we have created a relational database for managing and analysing information associated with the molecular genetics of this bacterium: Subtilist. It allows recovery of non-redundant DNA sequences of the B. subtilis genome, as well as related information, i.e. genes, proteins, etc. A logical structure has been designed with appropriate links between the different objects, and a set of procedures has been implemented for data updating and management. The database is organized around a core constituted by all known contigs of B. subtilis, i.e. sets of nonredundant sequences created from original entries in the EMBL data library. A user-friendly interface has been developed to make the database easy to consult. Sequence analysis tools have been integrated into the database, such as a program for rapid similarity searching of protein data banks, and a powerful DNA pattern searching program. Thanks to the consistency of Subtilist, we have performed a codon usage analysis by Factorial Correspondence Analysis, and a study of the distribution of the isoelectric points of known proteins of B. subtilis. The Subti List database is available through anonymous ftp (address ‘ftp.pasteur.fr’ or IP number 157.99.64.12, directory ‘/pub/GenomeDB/SubtiList’", }