1887

Abstract

Strains of subsp. small colony (SC) type, the agent of contagious bovine pleuropneumonia (CBPP), were analysed with respect to the polymorphism of distribution of a newly discovered insertion element, IS1296, on the chromosome. Analysis of 64 strains isolated from Europe, Africa and Australia, including four vaccine strains and the type strain PG1, revealed ten different IS patterns, forming two main clusters. The European strains originated from outbreaks of CBPP in different countries, and from various other sources such as semen and preputial washings from cattle, lungs from goats and buffalo, and milk from sheep. They showed identical IS1296 patterns, except one strain which had an additional IS1296 element, but the pattern belonged to the same cluster. This shows that the strains from Europe form a clonal lineage. The strains originating from different geographical parts of the African continent and from Australia showed four closely related IS1296 patterns which belong to a separate cluster. This indicates that strains from Africa and Australia form a clonal lineage different from that of the European strains, suggesting that the sporadic cases of CBPP that have re-emerged in Europe almost 15 years after the last declared endemic case in 1967 arose from an established reservoir within Europe rather than being the result of repeated importation from Africa and Australia. While most strains from Africa and Australia had the same IS1296 pattern, all vaccine strains could be distinguished by an individual pattern. The type strain PG1 also had a particular IS1296 pattern which belongs to the cluster of the strains from Africa and Australia. The molecular definition of clonality of subsp. SC strains with IS1296 represents a rapid and reproducible method for subtyping and differentiation of vaccine strains. It permits at the present time the definition of two main clonal lines, one including the strains from the European continent and a second with strains from Africa and Australia.

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1995-12-01
2024-03-28
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References

  1. Abu Groun E.A.M., Taylor R.R., Varsani H., Wadher B.J., Leach R.H., Miles R.J. 1994; Biochemical diversity within the ‘Mycoplasma mycoides’ cluster.. Microbiology 140:2033–2042
    [Google Scholar]
  2. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A. , Struhl K. 1990 Current Protocols in Molecular Biology. 1: New York: Wiley Interscience;
    [Google Scholar]
  3. Bannerman E.S., Nicolet J. 1971; Isolation and identification of porcine Mycoplasma in Switzerland.. Schweiz Arch Tierheilkd 113:697–710
    [Google Scholar]
  4. Bashiruddin J.B., Nicholas R.A.J., Santini F.G., Ready R.A., Woodward M.J., Taylor T.K. 1994a; Use of the polymerase chain reaction to detect mycoplasma DNA in cattle with contagious bovine pleuropneumonia.. Vet Rec 134:240–241
    [Google Scholar]
  5. Bashiruddin J.B., Taylor T.K., Gould A.R. 1994b; A PCR- based test for the specific identification of Mycoplasma mycoides subspecies mycoides SC.. J Vet Diagn Invest 6:428–434
    [Google Scholar]
  6. Brandao F. 1995; Isolation and identification of Mycoplasma mycoides subspecies mycoides SC strains in sheep and goats.. Vet Rec 136:98–99
    [Google Scholar]
  7. Brocchi E., Gamba D., Poumarat F., Martel J.L., De Simone F. 1993; Improvements in the diagnosis of contagious bovine pleuropneumonia through the use of monoclonal antibodies.. Rev Sci Tech Off Int Epiz 12:559–570
    [Google Scholar]
  8. Costas M., Leach R.H., Mitchelmore D.L. 1987; Numerical analysis of PAGE protein patterns and the taxonomic relationships within the ‘Mycoplasma mycoides cluster’.. J Gen Microbiol 133:3319–3329
    [Google Scholar]
  9. Cottew G.S., Breard A., DaMassa A.J., ErnØ H., Leach R.H., Lefevre P.C., Rodwell A.W., Smith G.R. 1987; Taxonomy of the Mycoplasma mycoides cluster.. Isr J Med Sci 23:632–635
    [Google Scholar]
  10. Dedieu L., Mady V., Lefevre P.C. 1994; Development of a selective polymerase chain reaction assay for the detection of Mycoplasma mycoides subsp.mycoides SC (contagious bovine pleuropneumonia agent).. Vet Microbiol 42:327–339
    [Google Scholar]
  11. Edward D.G., Freundt E.A. 1973; Type strains of species of the order Mycoplasmatales, including designation of neotypes for Mycoplasma mycoides subsp.mycoides, Mycoplasma agalactiae subsp.agalactiae, and Mycoplasma arthritidis. . Int J Syst Bacteriol 23:55–61
    [Google Scholar]
  12. van Embden J.D., Cave M.D., Crawford J.T., Dale J.W., Eisenach K.D., Gicquel B., Hermans P., Martin G, Shinnick T.M. 1993; Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology (see comments).. J Clin Microbiol 31:406–409
    [Google Scholar]
  13. Frey J., Cheng X., Kuhnert P., Nicolet J. 1995; Identification and characterization of IS 1296 in Mycoplasma mycoides subsp.mycoides SC and presence in related mycoplasmas.. Gene 160:95–100
    [Google Scholar]
  14. Galas D.J., Chandler M. 1989; Bacterial insertion sequences.. In Mobile DNA pp. 109–162 Berg D.E., Howe M.M. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Goncalves M.R. 1994; Isolation and identification of Mycoplasma mycoides subspecies mycoides SC from bull semen and sheath washings in Portugal.. Vet Rec 135:308–309
    [Google Scholar]
  16. Goncalves R., Regalia J., Penha-Goncalves A. 1994; Immuno- blotting and electrophoretic analysis of Mycoplasma mycoides subsp.mycoides SC strains isolated from bovine and small ruminants.. Int Organ Mycoplasmol Lett 3:64
    [Google Scholar]
  17. ter Laak E.A. 1992; Contagious bovine pleuropneumonia. A review.. Vet Q 14:104–110
    [Google Scholar]
  18. Nocard E.I.E., Roux E. 1898; Le microbe de la péripneumonie.. Ann Inst Pasteur 12:240–262 [Translated as ‘The microbe of pleuropneumonia’ in Rev Infect Dis 12,354-358(1990)]
    [Google Scholar]
  19. Piercy S.E., Knight G.J. 1956; Studies with avianised strains of the organism of contagious bovine pleuro-pneumonia.. Vet Rec 68:367–373
    [Google Scholar]
  20. Poumarat F. 1994; Molecular epidemiology of Mycoplasma mycoides subsp.mycoides biotype ‘small colony’ (M.m.m. SC).. Int Organ Mycoplasmol Lett 3:23
    [Google Scholar]
  21. Provost A. 1969; Principes de production d'un vaccin mixte associe antibovipestique-antiperipneumonique inocule en un seul temps.. Bull Epizoot Dis Afr 17:7–10
    [Google Scholar]
  22. Pyle L.E., Taylor T., Finch L.R. 1990; Genomic maps of some strains within the Mycoplasma mycoides cluster.. J Bacteriol 172:7265–7268
    [Google Scholar]
  23. Small P.M., Hopewell P.C, Singh S.P., Paz A., Parsonnet J., Ruston D.G, Schecter G.F., Daley GL., Schoolnik G.K. 1994; The epidemiology of tuberculosis in San Francisco. A population-based study using conventional and molecular methods.. N Engl J Med 330:1703–1709
    [Google Scholar]
  24. Stanley J., Baquar N., Threlfall E.J. 1993; Genotypes and phylogenetic relationships of Salmonella typhimurium are defined by molecular fingerprinting of IS200 and 16S rrn loci.. J Gen Microbiol 139:1133–1140
    [Google Scholar]
  25. Taylor T.K., Bashiruddin J.B., Gould A.R. 1992; Relationships between members of the Mycoplasma mycoides cluster as shown by DNA probes and sequence analysis.. Int J Syst Bacteriol 42:593–601
    [Google Scholar]
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