1887

Microbiology Society logo

Volume 141, Issue 12

Adaptive response of to hydrogen peroxide and menadione Free

Abstract

The response of to oxidative stresses has been examined. On challenging for 60 min at early exponential phase with either 40 mM HO or 6 mM menadione (MD), a superoxide-generating agent, less than 10% of the cells survived. Pretreating cells with 0.2 mM HO or 0.2 mM MD for 1 h significantly increased survival of these lethal doses of each oxidant, indicating the existence of an adaptive response to oxidative stress. Furthermore, cells pretreated with a low dose of MD became resistant to a lethal dose of HO. However, cells pretreated with HO became only partially resistant to a lethal dose of MD. Adaptation was accompanied by the induction of several oxidative defence enzymes. The presence of 0/2 mM HO induced catalase by 2.8-fold and peroxidase by 2.0-fold. The presence of 0.2 mM MD induced catalase by 2.0-fold, glucose-6-phosphate dehydrogenase by 1.9-fold, glutathione reductase by 2.7-fold, peroxidase by 3.0-fold, and superoxide dismutase (SOD) by 2.1-fold. The higher induction of these defence enzymes by MD may explain why MD-pretreated cells were better adapted to lethal doses of oxidants than HO-pretreated ones. All these enzymes except SOD and peroxidase increased more than 5.0-fold as cells proceeded into stationary phase. The GSH/GSSG ratio also increased by 60%. These changes accord with the observation that stationary phase cells survive oxidant treatment better than cells in vegetative growth.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): adaptive response , hydrogen peroxide , menadione , oxidative stress and Schizosaccharomyces pombe
Society for General Microbiology 1995
Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-141-12-3127
1995-12-01
2024-10-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/141/12/mic-141-12-3127.html?itemId=/content/journal/micro/10.1099/13500872-141-12-3127&mimeType=html&fmt=ahah

References

  1. Allen J.F. 1993; Redox control of transcription: sensors, response regulators, activators and repressors.. FEBS Lett 332:203–207
     [Google Scholar]
  2. Beers R.F. Jr Sizer I.W. 1952; A spectrophotometric method for measuring breakdown of hydrogen peroxide by catalase.. J Biol Chem 195:276–287
     [Google Scholar]
  3. Belaázzi T., Wieser R., Schanz M., Adam G., Ruis H. 1991; Negative regulation of transcription of the Saccharomyces cerevisiae catalase T (CTT1) gene by cAMP is mediated by a positive control element.. EMBO J 10:585–592
     [Google Scholar]
  4. Bol D.K., Yasbin R.E. 1990; Characterization of an inducible oxidative stress system in Bacillus subtilis. . J Bacteriol 172:3503–3506
     [Google Scholar]
  5. Bradford M.M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding.. Anal Biochem 72:248–254
     [Google Scholar]
  6. Christman M.F., Morgan R.W., Jacobson F.S., Ames B.N. 1985; Positive control of a regulon for defences against oxidative stress and some heat-shock proteins in Salmonella typhimurium. . Cell 41:753–762
     [Google Scholar]
  7. Collinson L.P., Dawes I.W. 1992; Inducibility of the response of yeast cells to peroxide stress.. J Gen Microbiol 138:329–335
     [Google Scholar]
  8. Decker L.A. 1977 Worthington Enzyme Manual New Jersey, USA: Worthington Biochemical Corporation;
     [Google Scholar]
  9. Dowds B.C.D., Murphy P., McConnell D.J., Devine K.M. 1987; Relationship among oxidative stress, growth cycle, and sporulation in Bacillus subtilis. . J Bacteriol 169:5771–5775
     [Google Scholar]
  10. Farr S.B., Kogoma T. 1991; Oxidative stress responses in Escherichia coli and Salmonella typhimurium. . Microbiol Rev 55:561–585
     [Google Scholar]
  11. Flattery-O’Brien J., Collinson L.P., Dawes I.W. 1993; Saccharomyces cerevisiae has an inducible response to menadione which differs from that to hydrogen peroxide.. J Gen Microbiol 139:501–507
     [Google Scholar]
  12. Galiazzo F., Labbe-Bois R. 1993; Regulation of Cu,Zn- and Mn-superoxide dismutase transcription in Saccharomyces cerevisiae. . FEBS Lett 315:197–200
     [Google Scholar]
  13. Gralla E.B., Kosman D.J. 1992; Molecular genetics of superoxide dismutases in yeast and related fungi.. In Advances in Genetics 30 pp 251–319 Edited by Scandalios J. G. San Diego, SF: Academic Press;
     [Google Scholar]
  14. Griffith O.W. 1980; Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine.. Anal Biochem 106:207–212
     [Google Scholar]
  15. Hidalgo E., Demple B. 1994; An iron-sulfur center essential for transcriptional activation by the redox-sensing SoxR protein.. EMBO J 13:138–146
     [Google Scholar]
  16. Hörtner H., Ammerer G., Hartter E., Hamilton B., Rytika J., Blinski T., Ruis H. 1982; Regulation of synthesis of catalase in Saccharomyces cerevisiae by glucose, oxygen and heme.. Eur J Biochem 128:179–184
     [Google Scholar]
  17. Imlay J.A., Linn S. 1988; DNA damage and oxygen radical toxicity.. Science 240:1302–1309
     [Google Scholar]
  18. Jamieson D.J. 1992; Saccharomyces cerevisiae has distinctive adaptive responses to both hydrogen peroxide and menadione.. J Bacteriol 174:6678–6681
     [Google Scholar]
  19. Jamieson D.J., Rivers S.L., Stephen D.W.S. 1994; Analysis of Saccharomyces cerevisiae proteins induced by peroxide and superoxide stress.. Microbiology 140:3277–3283
     [Google Scholar]
  20. Janda S., Gill G., Sigler K, Höfer M. 1993; Effects of peroxidation on sugar transport in Schiyosaccharomyces pombe. Absence of membrane lipid peroxidation.. Folia Microbiol 38:135–140
     [Google Scholar]
  21. Kletzien R.F., Harris P.K.W., Foellmi L.A. 1994; Glucose-6- phosphate dehydrogenase: a ‘housekeeping’ enzyme subject to tissue-specific regulation by hormones, nutrients, and oxidative stress.. FASEB J 8:174–181
     [Google Scholar]
  22. Kuge S., Jones N. 1994; YAP1 dependent activation of TRX2 is essential for the response of Saccharomyces cerevisiae to oxidative stress by hydroperoxides.. EMBO J 13:655–664
     [Google Scholar]
  23. Lange R., Hengge-Aronis R. 1991; Identification of a central regulator of stationary-phase gene expression in Escherichia coli. . Mol Microbiol 5:49–59
     [Google Scholar]
  24. Lee J.-S., Hah Y.-C., Roe J.-H. 1993; The induction of oxidative enzymes in Streptomyces coelicolor upon hydrogen peroxide treatment.. J Gen Microbiol 139:1013–1018
     [Google Scholar]
  25. Lowen P.C., Switala J., Triggs-Lain L. 1985; Catalase HPI and HPII in Escherichia coli are inducible independently.. Arch Biochem Biophys 243:144–149
     [Google Scholar]
  26. McCann M.P., Kidwell J.P., Matin A. 1991; The putative a factor KatF has a central role in development of starvation- mediated general resistance in Escherichia coli. . J Bacteriol 173:4188–4194
     [Google Scholar]
  27. McDonald I.J., Tsai C.S. 1989; Continuous culture and intermediary carbon metabolism.. In Molecular Biology of the Fission Yeast pp. 367–397 Edited by Nasim A., Young P., Johnson B. San Diego, SF: Academic Press;
     [Google Scholar]
  28. Moreno S., Klar A., Nurse P. 1991; Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. . Methods Encymol 194:795–823
     [Google Scholar]
  29. Mukhopadhyay S., Schellihorn H. 1994; Induction of Escherichia coli hydroperoxidase I by acetate and other weak acids.. J Bacteriol 176:2300–2307
     [Google Scholar]
  30. Paoletti F., Mocali A. 1993; Determination of superoxide dismutase activity by purely chemical system based on NAD(P)H oxidation.. In Oxygen Radicals in Biological Systems pp. 209–221 Edited by Packer L., Glazer A. N. San Diego, SF: Academic Press;
     [Google Scholar]
  31. Ruis H., Hamilton B. 1992; Regulation of yeast catalase genes.. In Molecular Biology of Free Radical Scavenging Systems pp. 153–172 Edited by Scandalios J. G. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press;
     [Google Scholar]
  32. Schnell N., Krems B., Entian K.-D. 1992; The PARI (YAP1 /SNQ3) gene of Saccharomyces cerevisiae, a c-jun homologue, is involved in oxygen metabolism.. Curr Genet 21:269–271
     [Google Scholar]
  33. Smith I.K., Vierheller T.L., Thorne CA. 1988; Assay of glutathione reductase in crude tissue homogenates using 5,5´- dithiobis(2-nitrobenzoic acid).. Anal Biochem 175:408–413
     [Google Scholar]
  34. Spitz D.R., Dewey W.G, Li G.C. 1987; Hydrogen peroxide or heat shock induces resistance to hydrogen peroxide in Chinese hamster fibroblasts.. J Cell Physiol 131:364–373
     [Google Scholar]
  35. Steels E.L., Learmonth R.P., Watson K. 1994; Stress tolerance and membrane lipid unsaturation in Saccharomyces cerevisiae grown aerobically or anaerobically.. Microbiology 140:569–576
     [Google Scholar]
  36. Storz G., Tartaglia L.A., Ames B.N. 1990a; Transcriptional regulator of oxidative stress-inducible genes: direct activation by oxidation.. Science 248:189–194
     [Google Scholar]
  37. Storz G., Tartaglia L.A., Farr S.B., Ames B.N. 1990b; Bacterial defences against oxidative stress.. Trends Genet 6:363–368
     [Google Scholar]
  38. Zitomer R.S., Lowry C.V. 1992; Regulation of gene expression by oxygen in Saccharomyces cerevisiae. . Microbiol Rev 56:1–11
     [Google Scholar]
/content/journal/micro/10.1099/13500872-141-12-3127
Loading
Adaptive response of Schizosaccharomyces pombe to hydrogen peroxide and menadione
Microbiology 141, 3127 (1995); https://doi.org/10.1099/13500872-141-12-3127
/content/journal/micro/10.1099/13500872-141-12-3127
/content/journal/micro/10.1099/13500872-141-12-3127
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error