1887

Abstract

A recombinant strain was constructed that constitutively produces two proteins from , the cytoplasmic -galactosidase and the periplasmic pH 2.5 acid phosphatase (AppA protein). We have previously shown that during vegetative growth, AppA protein is partly accumulated in the periplasm of and partly released into the medium. We demonstrate here that during starvation-induced development, release of periplasmic AppA protein to the medium did not occur over a period of 20 h. This was coincident with, but not caused by, the arrest of the synthesis of the foreign proteins. We have shown that this lack of secretion could be attributed to starvation and did not depend on the ability of the cells to undergo development. Our findings suggest that protein secretion which occurs during the first hours of starvation-induced development might therefore take place via a different route from that which occurs in vegetative cells.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-141-12-3119
1995-12-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/141/12/mic-141-12-3119.html?itemId=/content/journal/micro/10.1099/13500872-141-12-3119&mimeType=html&fmt=ahah

References

  1. Breton A., Guespin-Michel J.F. 1987; E. coli pH 2·5 acid phosphatase and βMactamase TEM-2 are secreted into the medium by Myxococcus xanthus.. FEMS Microbiol Lett 40:183–188
    [Google Scholar]
  2. Breton A.M., Younes G., VanGijsegen F., Guespin-Michel J.F. 1986; Expression in Myxococcus xanthus of foreign genes coding for secreted pectate lyases of Erwinia chrjsanthemi.. J Biotechnol 4:303–311
    [Google Scholar]
  3. Bretscher A.P., Kaiser D. 1978; Nutrition of Myxococcus xanthus, a fruiting myxobacterium.. J Bacteriol 133:763–768
    [Google Scholar]
  4. Casadaban M., Cohen S.N. 1980; Analysis of gene control signals by DNA fusion cloning in Escherichia coli.. J Mol Biol 138:179–207
    [Google Scholar]
  5. Cronan J.E. 1968; Phospholipid alterations during growth of Escherichia coli.. J Bacteriol 95:2054–2061
    [Google Scholar]
  6. Dagert M., Ehrlich S.D. 1974; Prolonged incubation in calcium chloride improves competence of Escherichia coli cells.. Gene 6:23–28
    [Google Scholar]
  7. Dassa E., Boquet P.L. 1981; expA: a conditional mutation affecting the expression of a group of exported proteins in Escherichia coli K-12.. Mol & Gen Genet 181:192–200
    [Google Scholar]
  8. Dassa J., Marck C., Boquet P.L. 1990; The complete nucleotide sequence of the Escherichia coli gene appA reveals significant homology between pH 2·5 acid phosphatase and glucose-1-phosphatase.. J Bacteriol 172:5497–5500
    [Google Scholar]
  9. Douillard D., Ribadeau-Dumas B. 1970; Détermination avec la K-caseine de l’activité protéolytique de la présure, de la pepsine de pore et des pepsines bovines.. Bull Soc Chim Biol 52:1429–1445
    [Google Scholar]
  10. Downard J., Kroos L. 1993; Transcriptional regulation of developmental gene expression in Myxococcus xanthus.. In Myxobacteria II pp. 183–199 Edited by Dworking M., Kaiser D. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  11. Guespin-Michel J.F., Letouvet-Pawlak B., Petit F. 1993; Protein secretion in myxobacteria.. In Myxobacteria II pp. 235–255 Edited by Dworkin M., Kaiser D. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  12. Hanahan D. 1983; Studies on transformation of Escherichia coli with plasmids.. J Mol Biol 166:557–580
    [Google Scholar]
  13. He S.Y., Lindeberg M., Chatterjee A.K., Collmer A. 1991; Cloned Erwinia chrysanthemi out genes enable Escherichia coli to selectively secrete a diverse family of heterologous proteins to its milieu.. Proc Natl Acad Sci USA 881079–1083
    [Google Scholar]
  14. Hodgson D.A. 1987; Light-inducible promoter. International Patent Application No. PCT/GB87/0040; New British Patent Application No. 8615263..
    [Google Scholar]
  15. Hodgson D.A. 1993; Light-induced carotenogenesis in Myxo- coccus xanthus-. genetic analysis of the carR region.. Mol Microbiol 7:471–488
    [Google Scholar]
  16. Holmes D.S., Quigley M. 1981; A rapid boiling method for the preparation of bacterial plasmids.. Anal Biochem 114:193–197
    [Google Scholar]
  17. Kaiser D. 1979; Social gliding is correlated with the presence of pili in Myxococcus xanthus.. Proc Natl Acad Sci USA 765952–5956
    [Google Scholar]
  18. Kaiser D. 1989; Multicellular development in myxobacteria.. In Genetics of Bacterial Diversity pp. 243–246 Edited by Hopwood D.A., Chater K.F. San Diego: Academic Press;
    [Google Scholar]
  19. Kuner J.H., Kaiser D. 1982; Fruiting body morphogenesis in submerged cultures of Myxococcus xanthus.. J Bacteriol 151:458–461
    [Google Scholar]
  20. Kuspa A.L., Kaiser D. 1989; Genes required for developmental signalling in Myxococcus xanthus: three asg loci.. J Bacteriol 171:2762–2772
    [Google Scholar]
  21. Letouvet-Pawlak B., Monnier C., Barray S., Hodgson D.A., Guespin-Michel J.F. 1990; Comparison of β-galactosidase production by two inducible promoters in Myxococcus xanthus.. Res Microbiol 141:425–435
    [Google Scholar]
  22. Letouvet-Pawlak B., Barray S., Laval-Favre K., Guespin-Michel J.F. 1993; Kinetics of secretion of recombinant acid phosphatase by Myxococcus xanthus: a sensitive probe for the assay of protein translocation through the envelopes.. J Gen Microbiol 139:3243–3252
    [Google Scholar]
  23. Masson P.J., Guespin-Michel J.F. 1988; An extracellular blood-anticoagulant glycopeptide produced exclusively during vegetative growth by Myxococcus xanthus and other myxobacteria is not co-regulated with other extracellular macromolecules.. J Gen Microbiol 134:801–806
    [Google Scholar]
  24. Miller J.H. 1972 Experiments in Molecular Genetics pp. 352–355 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Millet J. 1970; Characterization of proteinases excreted by Bacillus subtilis Marburg strain during sporulation.. J Appl Bacteriol 33:207–219
    [Google Scholar]
  26. Nicaud J.M., Breton A., Younes G., Guespin-Michel J.F. 1984; Mutants of Myxococcus xanthus impaired in protein secretion: an approach to study of a secretory mechanism.. Appl Microbiol Biotechnol 20:344–350
    [Google Scholar]
  27. Nikaido H., Vaara M. 1985; Molecular basis of bacterial outer membrane permeability.. Microbiol Rev 49:1–32
    [Google Scholar]
  28. O´Connor K.A., Zusman D.R. 1988; Reexamination of the role of autolysis in the development of Myxococcus xanthus.. J Bacteriol 170:4103–4112
    [Google Scholar]
  29. Petit F., Guespin-Michel J.F. 1992; Production of an extracellular milk-clotting activity during development in Myxococcus xanthus.. J Bacteriol 174:5136–5140
    [Google Scholar]
  30. Petit F., Merah M., Monnier C., Guespin-Michel J.F. 1993; Mutations in two new loci that impair both extracellular protein production and development in Myxococcus xanthus.. J Bacteriol 175:4239–4244
    [Google Scholar]
  31. Plamann L., Kuspa A., Kaiser D. 1992; Proteins that rescue A- signal-defective mutants of Myxococcus xanthus.. J Bacteriol 174:3311–3318
    [Google Scholar]
  32. Pugsley A.P. 1993; The complete general secretory pathway in Gram-negative bacteria.. Microbiol Rev 57:50–108
    [Google Scholar]
  33. Salmond G.P.C., Reeves P.J. 1993; Membrane traffic wardens and protein secretion in Gram-negative bacteria.. Trends Biochem Sci 18:7–12
    [Google Scholar]
  34. Shimkets L.J. 1990; Social and developmental biology of the myxobacteria.. Microbiol Rev 54:473–501
    [Google Scholar]
  35. Shimkets L.J., Gill R.E., Kaiser D. 1983; Developmental cell interactions in Myxococcus xanthus and the spoC locus.. Proc Natl Acad Sci USA 801406–1410
    [Google Scholar]
  36. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7- derived system for insertion mutagenesis and sequencing with synthetic universal primers.. Gene 19:259–268
    [Google Scholar]
  37. Zusman D.R., Kotoski D.M., Cumsky M. 1978; Chromosome replication in Myxococcus xanthus.. J Bacteriol 133:122–129
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-141-12-3119
Loading
/content/journal/micro/10.1099/13500872-141-12-3119
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error