1887

Abstract

Summary: The structural components in cell walls of three mutants of a facultative alkaliphile, C-125, defective in certain cell-wall components were characterized in detail. The cell walls of the wild-type C-125 were thick and increased in thickness when grown at high pH. Electron microscopy showed that triple layers developed when the bacteria were grown in an alkaline environment. In contrast, cell walls of teichuronopeptide (TUP)-defective mutants consisted of a single layer. For both the wild-type and mutants, the cell-wall concentrations of the acidic structural polymers teichuronic acid and TUP increased with respect to peptidoglycan as culture pH increased. For all four strains, the anion content of their cell walls was the greatest at high pH. The cell-wall density of the negatively charged compounds (uronic acids plus L-glutamic acid) was calculated as about 3 and 9 equivalents (I cell wall region) for C-125 cells grown at pH 7 and 10, respectively. At high pH, the specific growth rates of the two TUP-defective mutants were much lower than those of the wild-type. It is concluded that increased levels of acidic polymers in the cell walls of alkaliphilic bacteria may be a necessary adaptation for growth at elevated pH.

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1995-11-01
2024-04-23
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References

  1. Aono R. 1985; Isolation and partial characterization of structural components of the walls of alkalophilic Bacillus strain C-125.. J Gen Microbiol 131:105–111
    [Google Scholar]
  2. Aono R. 1987; Characterization of structural component of cell walls of alkalophilic strain of Bacillus sp. C-125.. Biochem J 245:467–472
    [Google Scholar]
  3. Aono R. 1989; Characterization of cell wall components of the alkalophilic Bacillus strain C-125: identification of a polymer composed of polyglutamate and polyglucuronate.. J Gen Microbiol 135:265–271
    [Google Scholar]
  4. Aono R. 1995; Assignment of facultatively alkaliphilic Bacillus sp. C-125 to Bacillus lentus group 3.. Int J Syst Bacteriol 45:582–585
    [Google Scholar]
  5. Aono R., Horikoshi K. 1983; Chemical composition of cell walls of alkalophilic strains of Bacillus. . J Gen Microbiol 129:1083–1087
    [Google Scholar]
  6. Aono R., Ohtani M. 1990; Loss of alkalophily in cell-wall- component-defective mutants derived from alkalophilic Bacillus C- 125.. Biochem J 266:933–936
    [Google Scholar]
  7. Aono R., Sanada T. 1994; Hyper-autolysis of the facultative alkaliphile Bacillus sp. C-125 cells grown at neutral pH: culture-pH dependent cross-linking of the peptide moieties of the pepti- doglycan.. Biosci Biotechnol Biochem 58:2015–2019
    [Google Scholar]
  8. Aono R., Uramoto M. 1986; Presence of fucosamine in teichuronic acid of the alkalophilic Bacillus strain C-125.. Biochem J 233:291–294
    [Google Scholar]
  9. Aono R., Horikoshi K., Goto S. 1984; Composition of the peptidoglycan of alkalophilic Bacillus spp.. J Bacteriol 157:688–689
    [Google Scholar]
  10. Aono R., Ito M., Horikoshi K. 1992; Instability of the protoplast membrane of facultative alkaliphilic Bacillus sp. C-125 at alkaline pH values below the pH optimum for growth.. Biochem J 285:99–103
    [Google Scholar]
  11. Aono R., Ito M., Horikoshi K. 1993; Regeneration of protoplasts prepared from alkaliphilic strains of Bacillus spp.. Biosci Biotechnol Biochem 57:1597–1598
    [Google Scholar]
  12. Aono R., Ito M., Joblin K., Horikoshi K. 1994; Genetic recombination after cell fusion of protoplasts from the facultative alkaliphile Bacillus sp. C-125.. Microbiology 140:3085–3090
    [Google Scholar]
  13. Beutler H.O., Michal G. 1974; Glutamate, determination with glutamate dehydrogenase, diaphorase and tetrazolium salts.. In Methods of Ennymatic Analysis 2nd edn. 4 pp. 1708–1713 Bergmeyer H.U. Edited by Weinheim: Verlag Chemie;
    [Google Scholar]
  14. Davidson E.A. 1966; Analysis of sugars found in mucopolysaccharides.. Methods Ensymol 8:52–60
    [Google Scholar]
  15. Ito M., Tabata K., Aono R. 1994; Construction of a new teichuronopeptide-defective derivative from alkaphilic Bacillus sp. C-125 by cell fusion.. Biosci Biotechnol Biochem 58:2215–2277
    [Google Scholar]
  16. Kemper M.A., Urrutia M.M., Beveridge T.J., Koch A.L., Doyle R.J. 1993; Proton motive force may regulate cell wall- associated enzymes of Bacillus subtilis. . J Bacteriol 175:5690–5696
    [Google Scholar]
  17. Kitada M., Hashimoto M., Kudo T., Horikoshi K. 1994; Properties of two different Na+/H+ antiport systems in alkaliphilic Bacillus sp. strain C-125.. J Bacteriol 176:6464–6469
    [Google Scholar]
  18. Koch A.L. 1986; The pH in the neighborhood of membranes generating a protonmotive force.. J Theor Biol 120:73–84
    [Google Scholar]
  19. Mera M.U., Kemper M., Doyle R., Beveridge T.J. 1992; The membrane-induced proton motive force influences the metal binding ability of Bacillus subtilis cell wall.. Appl Environ Microbiol 58:3837–3844
    [Google Scholar]
  20. Stepka W. 1957; Identification of amino acids by paper chromatography.. Methods Enspymol 3:504–528
    [Google Scholar]
  21. Trevelyan W.E., Procter D.P., Harrison J.S. 1950; Detection of sugars on paper chromatograms.. Nature 166:444–445
    [Google Scholar]
  22. Wheat R.W. 1966; Analysis of hexosamines in bacterial polysaccharides by chromatographic procedures.. Methods Enzymol 8:60–78
    [Google Scholar]
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