1887

Abstract

Summary: Arylsulphatase activity was identified in cultures of the marine bacterium using methylumbelliferyl sulphate as substrate. In contrast with most other microbial arylsulphatases, arylsulphatase production in was not repressed by sulphate. The structural gene of arylsulphatase () was cloned and sequenced. An ORF of 984 bp was found, specifying a primary translation product of 328 amino acids with a molecular mass of 35797 Da. Arylsulphatase was partially purified from cell extracts of both and recombinant . Both the recombinant and native enzymes exhibited a pl of 5-5, a Michaelis constant for methylumbelliferyl sulphate of 68 μM, and a molecular mass of approximately 35000 Da in SDS-PAGE analysis. Secondary structure comparisons using hydrophobic cluster analysis suggest functional analogies between the arylsulphatase of , that of and a 33-5 kDa protein from . It is speculated that these proteins are all glycosulphohydrolases, involved with desulphatation of sulphated polysaccharides.

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1995-11-01
2021-07-28
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References

  1. Adachi T., Murooka Y., Harada T. 1975; Regulation of arylsulfatase synthesis by sulfur compounds in Klebsiella aerogenes. . J Bacteriol 121:29–35
    [Google Scholar]
  2. Akagawa-Matsushita M., Matsuo M., Koga Y., Yamasato K. 1992; Alteromonas atlantica sp. nov. and Alteromonas carrageenovora sp. nov., bacteria that decompose algal polysaccharides.. Int J Sjst Bacteriol 42:621–627
    [Google Scholar]
  3. Barbeyron T., Henrissat B., Kloareg B. 1994; The gene encoding the kappa-Carrageenase of Alteromonas carrageenovora is related to beta-1,3–1,4--glucanases.. Gene 139:105–109
    [Google Scholar]
  4. Baumann L., Baumann P., Mandel M., Allen R.D. 1972; Taxonomy of aerobic marine eubacteria.. J Bacteriol 110:402–429
    [Google Scholar]
  5. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA.. Nucleic Acids Res 7:1513–1523
    [Google Scholar]
  6. De Hostos E.L., Togasaki R.K., Grossman A. 1988; Purification and biosynthesis of a derepressible periplasmic arylsulfatase from Chlamjdomonas reinhardtii. . J Cell Biol 106:29–37
    [Google Scholar]
  7. De Hostos E.L., Schilling J., Grossman A.R. 1989; Stucture and expression of the gene encoding the periplasmic arylsulfatase of Chlamjdomonas reinhardtii. . Mol & Gen Genet 218:229–239
    [Google Scholar]
  8. De Vos P., Van Landschoot A., Segers P., Tytgat R., Gillis M., Bauwens M., Rossau R., Goor M., Pot B., Kersters K., Lizzaraga P., de Ley J. 1989; Genotypic relationships and taxonomic localisation of unclassified Pseudomonas and Pseudomonas-like strains by deoxyribonucleic acid: ribosomal ribonucleic acid hybridizations.. Int J Sjst Bacteriol 39:35–49
    [Google Scholar]
  9. Fitzgerald J.W. 1976; Sulfate ester formation and hydrolysis: a potentially important yet often ignored aspect of the sulfur cycle of aerobic soils.. Bacteriol Rev 40:698–721
    [Google Scholar]
  10. Fitzgerald J.W. 1977; Localization of arylsufatase in Pseudomonas C12B.. Appl Environ Microbiol 34:107–108
    [Google Scholar]
  11. George J.R., Fitzgerald J.W. 1981; Arylsulfatase from Pseudomonas sp. strain C12B: purification to homogeneity, immunological analysis, and physical properties.. J Bacteriol 145:1428–1431
    [Google Scholar]
  12. Harley C.B., Reynolds R.P. 1987; Analysis of E. coli promotor sequences.. Nucleic Acids Res 15:2343–2360
    [Google Scholar]
  13. Henderson M.J., Milazzo F.H. 1979; Arylsulfatase in Salmonella typhimurium: detection and influence of carbon source and tyramine on its synthesis.. J Bacteriol 139:80–87
    [Google Scholar]
  14. von Heijne G. 1985; Signal sequences: the limits of variation.. J Mol Biol 184:99–105
    [Google Scholar]
  15. Johnston K.H., McCandless E.L. 1973; Enzymic hydrolysis of the potassium chloride soluble fraction of carrageenan: properties of ‘ambda-carrageenases’ from Pseudomonas carrageenovora. . Can J Microbiol 19:779–788
    [Google Scholar]
  16. Knöss W., Glombitza K.W. 1993; A phenolsulphatase from the marine brown alga Cystoseira tamariscifolia. . Phytochemistry 32:1119–1123
    [Google Scholar]
  17. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature 227:680–685
    [Google Scholar]
  18. McLean M.W., Williamson F.B. 1979a; Kappa -carrageenase from Pseudomonas carrageenovora. . Eur J Biochem 93:553–558
    [Google Scholar]
  19. McLean M.W., Williamson F.B. 1979b; Glycosulphatase from Pseudomonas carrageenovora. Purification and some properties.. Eur J Biochem 101:497–505
    [Google Scholar]
  20. McLean M.W., Williamson F.B. 1981; Neocarratetraose 4-O- monosulfate β-hydrolase from Pseudomonas carrageenovora. . Eur J Biochem 113:447–456
    [Google Scholar]
  21. Maniatis T., Fritsch E.F., Sambrook J. 1982 In Molecular Cloning: a Eaboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Murooka Y., Higashiura T., Harada T. 1978; Genetic mapping of tyramine oxidase and arylsulfatase genes and their regulation in intergeneric hybrids of enteric bacteria.. J Bacteriol 136:714–722
    [Google Scholar]
  23. Murooka Y., Ishibashi K., Yasumoto M., Sasaki M., Sugino H., Azakami H., Yamashita M. 1990; A sulfur- and tyramineregulated Klebsiella aerogenes operon containing the arylsulfatase (atsA) gene and the atsB gene.. J Bacteriol 172:2131–2140
    [Google Scholar]
  24. Oka M., Murooka Y., Harada T. 1980; Genetic control of tyramine oxidase, which is involved in derepressed synthesis of arylsulfatase in Klebsiella aerogenes. . J Bacteriol 143:321–327
    [Google Scholar]
  25. Peters C., Schmidt B., Rommerskirch W., Rupp K., Zühlsdorf M., Vingron M., Meyer H.E., Pohlmann R., Von Figura K. 1990; Phylogenetic conservation of arylsulfatases. cDNA cloning and expression of human arylsulfatase B.. J Biol Chem 265:3374–3381
    [Google Scholar]
  26. Rammler D.H., Grado C., Fowler L.R. 1964; Sulfur metabolism of Aerobacter aerogenes. I. A repressible sulfatase.. Biochemistry 3:224–230
    [Google Scholar]
  27. Rosenberg M., Court D. 1979; Regulatory sequences involved in the promotion and termination of RNA transcription.. Annu Rev Genet 13:319–353
    [Google Scholar]
  28. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 745463–5467
    [Google Scholar]
  29. Sasaki H., Akasaka K., Shimada H., Shiroya T. 1987; Purification and characterization of arylsulfatase from sea urchin (.Hemicentrotus pulcherimus) embryos.. Comp Biochem Physiol B 88:147–152
    [Google Scholar]
  30. Sasaki H., Yamada K., Akasaka K., Kawasaki H., Suzuki K., Saito A., Sato M., Shimada H. 1988; cDNA cloning, nucleotide sequence and expression of the gene for arylsulfatase in the sea urchin (Hemicentrotuspulcherrimus) embryo.. Eur J Biochem 177:9–13
    [Google Scholar]
  31. Stein C., Gieselmann V., Kreysing J., Shmidt B., Polhmann R., Waheed A., Meyer H.E., O’Brien J.S., Von Figura K. 1989a; Cloning and expression of human arylsulfatase A. . J Biol Chem 264:1252–1259
    [Google Scholar]
  32. Stein C., Hille A., Seidel J., Rijnbout S., Waheed A., Schimdt B., Geuze H., Von Figura K. 1989b; Cloning and expression of human steroid-sulfatase. Membrane topology, glycosylation, and subcellular distribution in BHK-21 cells.. J Biol Chem 264:13865–13872
    [Google Scholar]
  33. Tinoco I., Borer P.N., Dengler B., Levine M.D., Uhlenbeck O.C, Crothers D. M., Cralla J. 1973; Improved estimation of secondary structure in ribonucleic acid.. Nature 246:40–41
    [Google Scholar]
  34. Weigl J., Yaphe W. 1966; Glycosulfatase of Pseudomonas carrageenovora: desulfation of dissaccharide from kappa-carrageenan.. Can J Microbiol 12:874–876
    [Google Scholar]
  35. Weigl J., Turvey J.R., Yaphe W. 1966; The enzymic hydrolysis of kappa-carrageenan with kappa-carrageenase from Pseudomonas carrageenovora. . Proc Int Seamed Symp 5329–332
    [Google Scholar]
  36. Yang Q., Angerer L.M., Angerer R.C. 1989; Structure and tissue-specific developmental expression of a sea urchin arylsulfatase gene.. Dev Biol 135:53–65
    [Google Scholar]
  37. Yaphe W., Baxter B. 1955; The enzymic hydrolysis of carrageenin.. Appl Microbiol 3:380–383
    [Google Scholar]
  38. Yaphe W., Morgan K. 1959; Enzymic hydrolysis of fucoidin by Pseudomonas carrageenovora. . Nature 189:761–762
    [Google Scholar]
  39. Yen P.H., Allen E., Marsh B., Mohandas T., Wang N., Taggart R.T., Shapiro L.J. 1987; Cloning and expression of steroid sulfatase cDNA and the frequent occurence of deletions in STS deficiency: implications for X-Y interchange.. Cell 49:443–454
    [Google Scholar]
  40. Zobell C.E. 1941; Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes.. J Mar Res 4:41–75
    [Google Scholar]
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