1887

Abstract

Summary: ferments carbohydrate substrates to acetate, ethanol and L-lactate. To block acetate production, mutants defective in acetate kinase and/or phosphotransacetylase were isolated after mutagenesis with -methyl-'-nitro--nitroguanidine by selection for fluoroacetate resistance and subsequent counterselection for growth on pyruvate. Upon growth on glucose, all mutants formed only minor amounts of acetate and markedly less ethanol than the wild-type. The mutants produced L-lactate as the main fermentation product, yielding up to 1-8 mol lactate (mol glucose) as compared to 0-7 mol lactate (mol glucose) obtained with the parent strain. Expression of the genes encoding acetate kinase and phosphotransacetylase was found to be negatively controlled by acetate.

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1995-11-01
2021-10-16
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References

  1. Ballongue J., Amine J., Petitdemange H., Gay R. 1986; Regulation of acetate kinase and butyrate kinase by acids in Clostridium acetohutylicum.. FEMS Microbiol Lett 35:295–301
    [Google Scholar]
  2. Brown T.D.K., Jones-Mortimer M.C, Kornberg H.L. 1977; The enzymic interconversion of acetate and acetyL-coenzyme A in Escherichia coli.. J Gen Microbiol 102:327–336
    [Google Scholar]
  3. Canganella F., Wiegel J. 1993; The potential of thermophilic Clostridia in biotechnology.. In The Clostridia and Biotechnology pp. 393–429 Woods D.R. Edited by Boston:: Butterworth-Heinemann.;
    [Google Scholar]
  4. Clark S.W., Bennett G.N., Rudolph F.B. 1989; Isolation and characterization of mutants of Clostridium acetohutylicum ATCC 824 deficient in acetoacetyL-CoA :acetate/butyrate-CoA-transferase and in other solvent pathway enzymes.. Appl Environ Microbiol 55:970–976
    [Google Scholar]
  5. Glusker J.P. 1971; Aconitase.. In The Enɀymes 5, 3rd edn. pp. 413–439 Boyer P.D. Edited by New York:: Academic Press.;
    [Google Scholar]
  6. Junelles A.-M., Janati-Idrissi R., El Kanouni A., Petitdemange H., Gay R. 1987; Acetone-butanol fermentation by mutants selected for resistance to acetate and butyrate halogen analogues.. Biotechnol Lett 9:175–178
    [Google Scholar]
  7. Jungermann K., Thauer R.K., Leimenstoll G., Decker K. 1973; Function of reduced pyridine nucleotide-ferredoxin oxido- reductases in saccharolytic clostridia.. Biochim Biophys Acta 305:268–280
    [Google Scholar]
  8. Kakuda H., Hosono K., Shiroishi K., Ichihara S. 1994; Identification and characterization of the ackA (acetate kinase A)-pta (phosphotransacetylase) operon and complementation analysis of acetate utilization by an ackA-pta deletion mutant of Escherichia coli.. J Biochem 116:916–922
    [Google Scholar]
  9. Latimer M.T., Ferry J.G. 1993; Cloning, sequence analysis and hyperexpression of the genes encoding phosphotransacetylase and acetate kinase from Methanosarcina thermophila.. J Bacteriol 175:6822–6829
    [Google Scholar]
  10. Le Ruyet P., Dubourguier H.C., Albagnac G., Prensier G. 1985; Characterization of Clostridium thermolacticum sp. nov., a hydrolytic thermophilic anaerobe producing high amounts of lactate.. System Appl Microbiol 6:196–202
    [Google Scholar]
  11. Lovitt R.W., Shen G.-J., Zeikus G.J. 1988; Ethanol production by thermophilic bacteria: biochemical basis for ethanol and hydrogen tolerance in Clostridium thermohydrosulfuricum.. J Bacteriol 170:2809–2815
    [Google Scholar]
  12. McCleary W.R., Stock J.B., Ninfa A.J. 1993; Is acetyl phosphate a global signal in Escherichia coli? . J Bacteriol 175:2793–2798
    [Google Scholar]
  13. Murray W.D., Wemyss K.B., Khan A.W. 1983; Increased ethanol production and tolerance by a pyruvate negative mutant of Clostridium saccharolyticum.. Eur J Appl Microbiol Biotechnol 18:71–74
    [Google Scholar]
  14. Oultram J.D., Burr I.D., Elmore M.J., Minton N.P. 1993; Cloning and sequence analysis of the genes encoding phospho- transbutyrylase and butyrate kinase from Clostridium acetohutylicum NCIMB 8052.. Gene 131:107–112
    [Google Scholar]
  15. Rogers P., Gottschalk G. 1993; Biochemistry and regulation of acid and solvent production in clostridia.. In The Clostridia and Biotechnology pp. 25–50 Woods D.R. Edited by Boston:: Butterworth-Heinemann.;
    [Google Scholar]
  16. Rothstein D.M. 1986; Clostridium thermosaccharolyticum strain deficient in acetate production.. J Bacteriol 165:319–320
    [Google Scholar]
  17. Sedmak J.J., Grossberg S.E. 1977; A rapid, sensitive and versatile assay for protein using Coomassie brilliant blue G 250.. Anal Biochem 79:544–552
    [Google Scholar]
  18. Turunen M., Parkkinen E., Londesborough J., Korhola M. 1987; Distinct forms of LDH purified from ethanoL- and lactate-producing cells of Clostridium thermohydrosulfuricum.. J Gen Microbiol 133:2865–2873
    [Google Scholar]
  19. Van Dyk T.K., LaRossa R.A. 1987; Involvement of ack-ptaoperon products in α-ketobutyrate metabolism by Salmonella typhimurium.. Mol & Gen Genet 207:435–440
    [Google Scholar]
  20. Walter K.A., Nair R.V., Cary J.W., Bennett G.N., Papoutsakis E.T. 1993; Sequence and arrangement of two genes of the butyrate-synthesis pathway of Clostridium acetobutylicum ATCC 824.. Gene 134:107–111
    [Google Scholar]
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