1887

Abstract

Summary: In order to examine the role of glutaminase in we isolated and characterized a glutaminase mutant (LM16). This mutant was selected for its impaired ability to grow on glutamine as nitrogen and carbon source while retaining the ability to grow on other nitrogen and carbon sources. The mutant showed very low levels of glutaminase activity under various growth conditions in comparison with the wild-type strain. With glutamine as the only nitrogen and carbon source, LM16 showed poor growth, with a very high content of glutamine, low glutamate content, and reduced ammonium excretion and CO evolution from [U-C]glutamine compared to the wild-type strain. This indicates that the main role of glutaminase is in the use of glutamine as carbon source. glutaminase also plays a role in maintaining the balance between glutamate and glutamine, as shown by the accumulation of glutamine and the low glutamate content of the mutant under different growth conditions. These results also indicate that glutaminase participates in a glutamine cycle in which it degrades glutamine which is then resynthesized by glutamine synthetase. The higher glutamine and lower glutamate content found in bacteroids of LM16 in comparison with bacteroids of the wild-type strain indicate that glutamine degradation by glutaminase plays an important role during the symbiosis between and .

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1995-11-01
2021-10-18
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References

  1. Bender R.A., Janssen K.S., Resnick A.D., Blumenberg M., Foor F., Magasanik B. 1977; Biochemical parameters of glutamine synthetase from Klebsiella aerogenes. . J Bacteriol 129:1001–1009
    [Google Scholar]
  2. Beringer J.E. 1974; R factor transfer in Rhizobium leguminosarum. . J Gen Microbiol 84:188–198
    [Google Scholar]
  3. Bravo A., Mora J. Ammonium assimilation in Rhixobium phaseoli by the glutamine synthetase-glutamate synthase pathway.. J Bacteriol 170:980–984
    [Google Scholar]
  4. Bravo A., Becerril B., Mora J. 1988; Introduction of the Escherichia coli gdhA gene into Rhiyobium phaseoli: effect on nitrogen fixation.. J Bacteriol 170:985–988
    [Google Scholar]
  5. de Bruijn F.J., Rossbach S., Scheider M., Ratet P., Mesmer S., Szeto W.W., Ausubel F.M., Schell J. 1989; Rhizobium meliloti1021 has three differentially regulated loci involved in glutamine biosynthesis, none of which is essential for symbiotic nitrogen fixation.. J Bacteriol 171:1673–1682
    [Google Scholar]
  6. Calderón J., Mora J. 1985; Glutamine cycling in Neurospora crassa. . J Gen Microbiol 131:3237–3242
    [Google Scholar]
  7. Calderón J., Mora J. 1989; Glutamine assimilation pathways in Neurospora crassa growing on glutamine as sole nitrogen and carbon source.. J Gen Microbiol 135:2699–2707
    [Google Scholar]
  8. Calderón J., Morett E., Mora J. 1985; ω-Amidase pathway in the degradation of glutamine in Neurospora crassa. . J Bacteriol 161:807–809
    [Google Scholar]
  9. Calderón J., Cooper A.J.L., Gelbard A.S., Mora J. 1989; 13N isotope studies of glutamine assimilation pathways in Neurospora crassa. . J Bacteriol 171:1772–1774
    [Google Scholar]
  10. Chaney A.L., Marbach R. 1962; Modified reagents for the determination of urea and ammonia.. Clin Chem 8:130–132
    [Google Scholar]
  11. Chiurazzi M., Meza R., Lara M., Lahn A., Defez R., laccarino M., Espin G. 1992; The Rhizobium leguminosarum biovar phaseoli glnTgene, encoding glutamine synthetase III.. Gene 119:1–8
    [Google Scholar]
  12. Darrow R.A., Knotts R.R. 1977; Two forms of glutamine synthetase in free-living root-nodule bacteria.. Biochem Biophys Res Commun 78:554–559
    [Google Scholar]
  13. Donald R.G.K., Ludwig R.A. 1984; Rhizobium sp. strain ORS571 ammonium assimilation and nitrogen fixation.. J Bacteriol 158:1144–1151
    [Google Scholar]
  14. Duran S., Calderón J. 1995; Role of the glutamine transaminase-ω-amidase pathway and glutaminase in glutamine degradation in Rhizobium etli. . Microbiology 141:589–595
    [Google Scholar]
  15. Espin G., Moreno S., Wild M., Meza R., laccarino M. 1990; A previously unrecognized glutamine synthetase expressed in Klebsiella pneumoniae from the glnT locus of Rhizobium leguminosarum. . Mol & Gen Genet 223:513–516
    [Google Scholar]
  16. Fuchs R.L., Keister D.L. 1980; Identification of two glutamine synthetases in Agrobacterium. . J Bacteriol 141:996–998
    [Google Scholar]
  17. Gibson A.H. 1980; Method for legumes in glasshouse and controlled environment cabinets.. In Methods for Evaluating Biological Nitrogen Fixation pp. 139–185 Bergersen F. J. Edited by New York:: John Wiley.;
    [Google Scholar]
  18. Halpern Y.S. 1988; Control of transport and utilization of nitrogen sources in bacteria.. In Nitrogen Source Control of Microbial Processes pp. 21–58 Sanchez-Esquivel S. Edited by Boca Raton, FL:: CRC Press.;
    [Google Scholar]
  19. Kumar P.S., Rao S.L.N. 1986; Identification and characterization of three forms of glutamine synthetase unique to Rhizobia.. Curr Microbiol 14:221–226
    [Google Scholar]
  20. Martin G.B., Chapman K.A., Chelm B.K. 1988; Role of the Bradyrhizobium japonicum ntrC gene product in differential regulation of the glutamine synthetase II gene (glnII).. J Bacteriol 170:5452–5459
    [Google Scholar]
  21. Mora J. 1990; Glutamine metabolism and cycling in Neurospora crassa. . Microbiol Rev 54:293–304
    [Google Scholar]
  22. Mora J., Salceda R., Sánchez S. 1972; Regulation of Neurospora crassa glutamine synthetase by carbon and nitrogen source.. J Gen Microbiol 118:455–463
    [Google Scholar]
  23. Osorio A., Camarena L., Salazar G., Noll-Louzada M. 1993; Nitrogen regulation in an Escherichia colistrain with a temperature sensitive glutamyl-tRNA synthetase.. Mol & Gen Genet 239:400–408
    [Google Scholar]
  24. Prusiner 5. 1973; Glutaminases of Escherichia coli: properties, regulation and evolution.. In The Enzymes of Glutamine Metabolism pp. 293–315 Prusiner S., Stadtman E. R. Edited by New York:: Academic Press.;
    [Google Scholar]
  25. Reibach P.H., Mask P.L., Streeter J.G. 1981; A rapid one-step method for the isolation of bacteroids from root nodules of soybean plants, utilizing self-generating Percoll gradients.. Can J Microbiol 27:491–495
    [Google Scholar]
  26. Rossbach S., Shell J., de Bruijn F.J. 1987; The ntrC of Agrobacterium tumefaciens C58 controls glutamine synthetase (GSII) activity, growth on nitrate and chromosomal but not Ti-encoded arginine catabolism pathways.. Mol & Gen Genet 209:419–426
    [Google Scholar]
  27. Rossi M., Defez R., Chiurazzi M., Lamberti A., Fuggi A., laccarino M. 1989; Regulation of glutamine isoenzymes in Rhizobium leguminosarum biovar viceae. . J Gen Microbiol 135:629–637
    [Google Scholar]
  28. Segovia L., Young P.W., Martinez-Romero E. 1993; Reclassification of American Rhizobium leguminosarum biovar phaseoli type I as Rhizobium etli sp. nov. . Int J Sjst Bacteriol 43:374–377
    [Google Scholar]
  29. Shatters R.G., Somerville J.E., Kahn M.L. 1989; Regulation of glutamine synthetase II activity in Rhizobium meliloti 104A14.. J Bacteriol 171:5087–5094
    [Google Scholar]
  30. Shatters R.G., Liu Y., Kahn M.L. 1993; Characterization of a novel glutamine synthetase from R. meliloti. . J Biol Chem 268:469–475
    [Google Scholar]
  31. Simon R. 1984; High frequency mobilization of gram-negative bacterial replicons by the in vitro constructed Tn5-Mob transposon.. Mol & Gen Genet 196:413–420
    [Google Scholar]
  32. Soberón M., González A. 1987; Glutamine degradation through the co-amidase pathway in Saccharomyces cerevisiae. . J Gen Microbiol 133:9–14
    [Google Scholar]
  33. Stadtman E.R. 1973; A note on the significance of glutamine in intermediary metabolism.. In The Enzymes of Glutamine Metabolism pp. 1–6 Prusiner S., Stadtman E. R. Edited by New York:: Academic Press.;
    [Google Scholar]
  34. Tsupurun U.L, Zograft O.N., Orlova E.V., Keiselev N.A., Pushkin A.V., Shiffelova G.E., Solovieva N.A., Evstigneeva S.G., Kretovich W.L. 1987; Electron microscopy of multi forms of glutamine synthetase from bacteroids and the cytosol of yellow Lupin root nodules.. Biochim Biophys Acta 913:368–376
    [Google Scholar]
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