1887

Abstract

Summary: The FimH adhesin of type 1 fimbriae has been tested as a display system for heterologous protein segments on the surface of . This was carried out by introduction of restriction site handles sites) in two different positions in the gene, followed by in-frame insertion of heterologous DNA segments encoding two reporter sequences. In the selected positions such insertions did not significantly alter the function of the FimH protein with regard to surface location and adhesive ability. The system seemed to be quite flexible, since chimeric versions of the FimH adhesin containing as many as 56 foreign amino acids were transported to the bacterial surface as components of the fimbrial organelles. Furthermore, the foreign protein segments were recognized by insert-specific antibodies when expressed within chimeric proteins on the surface of the bacteria. The results from this feasibility study point to the possibility of using the FimH adhesin as a general surface display system for sizeable protein segments.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-141-11-2839
1995-11-01
2021-07-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/141/11/mic-141-11-2839.html?itemId=/content/journal/micro/10.1099/13500872-141-11-2839&mimeType=html&fmt=ahah

References

  1. Abraham S.N., Gougen J.D., Sun D., Klemm P., Beachey E.H. 1987; Identification of two ancillary subunits of Escherichia coli type 1 fimbriae using antibodies against synthetic oligopeptides of fim gene products.. J Bacteriol 169:5530–5536
    [Google Scholar]
  2. Abraham S.N., Gougen J.D., Beachey E.H. 1988; Hyperadhesive mutant of type 1-fimbriated Escherichia coli associated with formation of FimH organelles (fimbriosomes).. Infect Immun 56:1023–1029
    [Google Scholar]
  3. Birnboim H.C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA.. Nucleic Acids Res 7:1513–1523
    [Google Scholar]
  4. Blomfield I.C., McClain M.S., Eisenstein B.I. 1991; Type 1 fimbriae mutants of Escherichia coli K12: characterization of recognized afimbriate strains and construction of new fim deletion mutants.. Mol Microbiol 5:1439–1445
    [Google Scholar]
  5. Boyer H.W., Roulland-Dussoix D. 1969; A complementary analysis of the restriction and modification of DNA in Escherichia coli.. J Mol Biol 41:459–472
    [Google Scholar]
  6. Brinton C.C. Jr 1965; The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in Gram negative bacteria.. Trans N Y Acad Sci 27:1003–1054
    [Google Scholar]
  7. Chang A.C.Y., Cohen S.N. 1978; Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.. J Bacteriol 134:1141–1156
    [Google Scholar]
  8. Chanteloup N.K., Dho-Moulin M., Esnault E., Brée A., Lafont J.-P. 1991; Serological conservation and location of the adhesin of avian Escherichia coli type 1 fimbriae.. Microb Pathog 10:271–280
    [Google Scholar]
  9. Charnay P., Pourcel C., Louise A., Fritsch A., Tiollais P. 1979; Cloning in Escherichia coli and physical structure of hepatitis B virion DNA.. Proc Natl Acad Sci USA 762222–2226
    [Google Scholar]
  10. Dodd D.C., Eisenstein B.I. 1984; Dependence of secretion and assembly of type 1 fimbrial subunits on normal protein export.. J Bacteriol 159:1077–1079
    [Google Scholar]
  11. Hanson M.S., Hempel J., Brinton C.C. Jr 1988; Purification of the Escherichia coli type 1 pilin and minor pilin proteins and partial characterization of the adhesin protein.. J Bacteriol 170:3350–3358
    [Google Scholar]
  12. Haslam D.B., Borén T., Falk P., Ilver D., Chou A., Xu Z., Normark S. 1994; The amino-terminal domain of the P-pilus adhesin determines receptor specificity.. Mol Microbiol 14:399–409
    [Google Scholar]
  13. Hasty D.L, Courtney H.S., Sokurenko E.V., Ofek I. 1994; Bacteria-extracellular matrix interactions.. In Fimbriae, Adhesion, Genetics, Biogenesis and Vaccines pp. 197–211 Klemm P. Edited by Boca Raton, FL:: CRC Press.;
    [Google Scholar]
  14. Hedegaard L., Klemm P. 1989; Type 1 fimbriae of Escherichia coli as carriers of heterologous antigenic sequences.. Gene 85:115–124
    [Google Scholar]
  15. Hultgren S.J., Abraham S.N., Caparon M., Falk P., St Geme J.W. III Normark S. 1993; Pilus and non pilus bacterial adhesins: assembly and function in cell recognition.. Cell 73:887–901
    [Google Scholar]
  16. Itoh Y., Takai E., Ohnuma H., Kitajama K., Tsuda F., Machida A., Mishiro S., Nakamura T., Miyakawa Y., Mayumi M. 1986; A synthetic peptide vaccine involving the product of the pre-S2 region of hepatitis B virus DNA: protective efficacy in chimpanzees.. Proc Natl Acad Sci USA 839174–9178
    [Google Scholar]
  17. Jacob O.C., Sela M., Arnon R. 1983; Antibodies against synthetic peptides of the B subunit of cholera toxin: crossreaction and neutralization of the toxin.. Proc Natl Acad Sci USA 807611–7615
    [Google Scholar]
  18. Jones C.H., Pinkner J.S., Nicholes A.V., Slonim L.N., Abraham S.N., Hultgren S.J. 1993; FimC is a periplasmic PapD-like chaperone that directs assembly of type 1 pili in bacteria.. Proc Natl Acad Sci USA 908397–8401
    [Google Scholar]
  19. Klemm P. 1992; FimC, a chaperone-like periplasmic protein of Escherichia coli involved in biogenesis of type 1 fimbriae.. Res Microbiol 143:831–838
    [Google Scholar]
  20. Klemm P., Christiansen G. 1987; Three fim genes required for the regulation of length and mediation of adhesion of Escherichia colitype 1 fimbriae.. Mol & Gen Genet 208:439–445
    [Google Scholar]
  21. Klemm P., Christiansen G. 1990; The fimD gene required for cell surface localization of Escherichia coli type 1 fimbriae.. Mol & Gen Genet 220:334–338
    [Google Scholar]
  22. Klemm P., Krogfelt K.A. 1994; Type 1 fimbriae of Escherichia coli. . In Fimbriae, Adhesion, Genetics, Biogenesis and Vaccines pp. 9–26 Klemm P. Edited by Boca Raton, FL:: CRC Press.;
    [Google Scholar]
  23. Klemm P., Jørgensen B.J., van Die I., de Ree H., Bergmans H. 1985; The fim genes responsible for synthesis of type 1 fimbriae in Escherichia coli.. Mol & Gen Genet 199:410–414
    [Google Scholar]
  24. Klemm P., Krogfelt K.A., Hedegaard L., Christiansen G. 1990; The major subunit of Escherichia coli type 1 fimbriae is not required for D-mannose specific adhesion.. Mol Microbiol 4:553–559
    [Google Scholar]
  25. Klemm P., Christiansen G., Kreft B., Marre R., Bergmans H. 1994; Reciprocal exchange of minor components of type 1 and F1C fimbriae results in hybrid organelles with changed receptor specificity.. J Bacteriol 176:2227–2234
    [Google Scholar]
  26. Krogfelt K.A. 1991; Bacterial adhesion: genetics, biogenesis, and role in pathogenesis of fimbrial adhesins of Escherichia coli.. Rev Infect Dis 13:721–735
    [Google Scholar]
  27. Krogfelt K.A., Klemm P. 1988; Investigation of minor components of Escherichia coli type 1 fimbriae: protein chemical and immunological aspects.. Microb Pathog 4:231–238
    [Google Scholar]
  28. Pallesen L., Klemm P. 1994; Chimeric fimbrial vaccines.. In Fimbriae, Adhesion, Genetics, Biogenesis and Vaccines pp. 271–276 Klemm P. Edited by Boca Raton, FL:: CRC Press.;
    [Google Scholar]
  29. Pfaff E.P., Klinkert M.-Q., Theilmann L., Schaller H. 1986; Characterization of large surface proteins of hepatitis B virus by antibodies to preS-S encoded amino acids.. Virology 148:15–22
    [Google Scholar]
  30. Pugsley A.P. 1993; The complete secretory pathway in Gramnegative bacteria.. Microbiol Rev 57:50–108
    [Google Scholar]
  31. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 745463–5467
    [Google Scholar]
  32. Sokurenko E.V., Courtney H.S., Abraham S.N., Klemm P., Hasty D.L. 1992; Functional heterogeneity of type 1 fimbriae of Escherichia coli.. Infect Immun 60:4709–4719
    [Google Scholar]
  33. Sokurenko E.V., Courtney H.S., Ohman D.E., Klemm P., Hasty D.L. 1994; FimH family of type 1 fimbrial adhesins: functional heterogeneity due to minor sequence variations among fimH genes.. J Bacteriol 176:748–755
    [Google Scholar]
  34. Tabor S., Richardson C. 1985; A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.. Proc Natl Acad Sci USA 821074–1078
    [Google Scholar]
  35. Tiollais P., Pourcel C., Dejan A. 1985; The hepatitis B virus.. Nature 317:489–495
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-141-11-2839
Loading
/content/journal/micro/10.1099/13500872-141-11-2839
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error