1887

Abstract

Summary: A few entomophthoralean species are able to multiply in a protoplast form. The polysaccharide synthases which synthesize the cell wall are inactivated in this form. An inhibitor of one of the key enzymes of wall synthesis, glucan synthase, was isolated from entomophthoralean protoplasts, using silica column chromatography and HPLC. Thin-layer and gas chromatography revealed free fatty acids in the inhibitory fractions. These fatty acids, including long-chain unsaturated fatty acids, were shown to be responsible for the inhibition of glucan synthase. The fatty acids were generated during incubation of a protoplast homogenate for 36 h at 37 °C and were shown to be non-competitive and non-specific inhibitors of glucan synthase.

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1995-10-01
2021-10-23
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References

  1. Beaulieu D., Tang J., Zeckner D., Parr T. 1993; Correlation of cilofungin in vivo efficacy with its activity against Aspergillus Jumigatus (l,3)-β-D-glucan synthase.. FEMS Microbiol Lett 108:133–138
    [Google Scholar]
  2. Beauvais A., Latgé J.P. 1988; A simple medium to grow Entomophthoralean protoplasts.. J Invertebr Pathol 51:175–178
    [Google Scholar]
  3. Beauvais A., Latgé J.P. 1989; Chitin and β(l-3)glucan synthases in the protoplastic entomophthorales.. Arch Microbiol 152:229–236
    [Google Scholar]
  4. Beauvais A., Latgé J.P., Vey A., Prevost M.C. 1989; The role of surface components of the entomopathogenic fungus Entomophaga aulicae in the cellular immune response of Galleria mellonella (Lepidoptera).. J Gen Microbiol 135:489–498
    [Google Scholar]
  5. Beauvais A., Drake R., Ng K., Diaquin M., Latgé J.P. 1993; Characterization of the 1,3-β-glucan synthase of Aspergillus fumigatus.. J Gen Microbiol 139:3071–3078
    [Google Scholar]
  6. Cabib E., Bowers B., Sburlati A., Silverman S.J. 1988; Fungal cell wall synthesis; the construction of a biological structure.. Microbiol Sci 5:370–375
    [Google Scholar]
  7. Fevre M. 1979; Glucanases, glucan synthases and wall growth in Saprolegnia monoica.. In Fungal Walls and Fljphal Growth pp. 225–263 Burnett J.H., Trinci A.P.J. Edited by Cambridge:: Cambridge University Press.;
    [Google Scholar]
  8. Fromtling R., Abruzzo G. 1989; L-671,329, a new antifungal agent: in vitro activity, toxicity and efficacy in comparison to aculeacin.. J Antibiot 42:174–178
    [Google Scholar]
  9. Hamilton J., Era S., Bhamidipati S., Reed R. 1991; Locations of the three primary binding sites for long-chain fatty acids on bovine serum albumin.. Proc Natl Acad Sci USA 882051–2054
    [Google Scholar]
  10. Higgins J.A. 1987; Separation and analysis of membrane lipid components.. In Biological Membranes: a Practical Approach pp. 103–137 Findlay J.B.C., Evans W.H. Edited by Oxford:: IRL Press.;
    [Google Scholar]
  11. Iwata K., Yamamoto Y., Yamaguchi H., Hiratani T. 1982; In vitro studies of Aculeacin A, a new antifungal antibiotic.. J Antibiot 35:203–209
    [Google Scholar]
  12. Kang M.S., Szaniszlo P.J., Notario V., Cabib E. 1986; The effect of Papulacandin B on (l-3)-β-D-glucan synthetases. A possible relationship between inhibition and enzyme conformation.. Carbohydr Res 149:13–21
    [Google Scholar]
  13. Kates M. (editor) 1986 Techniques of Eipidology: Isolation, Analysis and Identification of Lipids. Amsterdam:: Elsevier;
    [Google Scholar]
  14. Kerwin J.L., Tuininga A.R., Ericsson L.H. 1994; Identification of molecular species of glycerophospholipids and sphingomyelin using electrospray mass spectrometry.. J Lipid Res 35:1102–1114
    [Google Scholar]
  15. Ko Y.T., Frost D., Ho C.T., Ludescher R., Wasserman B. 1993; Inhibition of yeast (1,3)-β-glucan synthase by phosphoUpase A2 and its reaction products.. Biochim Biophys Acta 1193:31–40
    [Google Scholar]
  16. Maresca B., Carratu L., Kobayashi G.S. 1994; Morphological transition in the human fungal pathogen Histoplasma capsulatum.. Trends Microbiol 2:110–114
    [Google Scholar]
  17. Sawistowska-Schroder E.T., Kerridge D., Perry H. 1984; Echinocandin inhibition of l,3-β-D-glucan synthase from Candida albicans.. FEBS Lett 173:134–138
    [Google Scholar]
  18. Taft C.S., Seletrennikoff C.P. 1990; Cilofungin inhibition of (l-3)-)β-glucan synthase: the lipophilic side chain is essential for inhibition of enzyme activity.. J Antibiot 43:433–437
    [Google Scholar]
  19. Thomsen L., Beauvais A. 1995; Cloning of two chitin synthase gene fragments from a protoplastic entomophthorale.. FEMS Microbiol Lett 129:115–120
    [Google Scholar]
  20. Varona R., Perez P., Duran A. 1983; Effect of papulacandin B on β-glucan synthesis in Sshɀosaccharomyces pombe.. FEMS Microbiol Lett 20:243–247
    [Google Scholar]
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