1887

Abstract

Summary: Gel-stabilized two-dimensional gradient plates were used to study the effects of pH, salt concentration and temperature on the conjugal transfer of plasmid RP4 between strains of and . The combinations of pH and salt concentration that permitted conjugation were mapped as a two-dimensional growth area occupied by transconjugants following conjugation. This conjugation domain was less extensive than the areas that supported growth of the parental strains, and showed evidence for the interactive effects of pH and salt concentration in determination of conditions that permitted conjugation. The size and shape of the conjugation domain was influenced by time, temperature, the identities of the donor and recipient bacteria, and the combination of donor and recipient bacteria.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-141-10-2713
1995-10-01
2022-01-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/141/10/mic-141-10-2713.html?itemId=/content/journal/micro/10.1099/13500872-141-10-2713&mimeType=html&fmt=ahah

References

  1. Fernandez-Astorga A., Muela A., Cisterna R., Iriberri J., Barcina I. 1992; Biotic and abiotic factors affecting plasmid transfer in Escherichia coli strains.. Appl Environ Microbiol 58:392–398
    [Google Scholar]
  2. Gauthier M.J., Cauvin F., Breittmayer V.A. 1985; Influence of salts and temperature on the transfer of mercury resistance from a marine pseudomonad to Escherichia coli.. Appl Environ Microbiol 50:38–40
    [Google Scholar]
  3. Kelly W.J., Reanney D.C. 1984; Mercury resistance among soil bacteria: ecology and transferability of genes encoding resistance.. Soil Biol Biochem 16:1–8
    [Google Scholar]
  4. Khalil T.A., Gealt M.A. 1987; Temperature, pH and cations affect the ability of Escherichia coli to mobilize plasmids in L-broth and synthetic wastewater.. Can J Microbiol 33:733–737
    [Google Scholar]
  5. MacDonald J.A., Smets B.F., Rittman B.E. 1992; The effects of energy availability on the conjugative-transfer kinetics of plasmid RP4.. Water Res 26:461–468
    [Google Scholar]
  6. Rochelle P.A., Fry J.C., Day M.J. 1989; Factors affecting conjugal transfer of plasmids encoding mercury resistance from pure cultures and mixed natural suspensions of epilithic bacteria.. J Gen Microbiol 135:409–424
    [Google Scholar]
  7. Singleton P., Anson A.E. 1981; Conjugal transfer of R-plasmid R1drd-19 in Escherichia coli below 22 °C.. Appl Environ Microbiol 42:789–791
    [Google Scholar]
  8. Sybalski W., Bryson V. 1952; Cross-resistance of Escherichia colito fifteen antibiotics.. J Bacteriol 64:489–499
    [Google Scholar]
  9. Trevors J.T., Oddie K.M. 1986; R-plasmid transfer in soil and water.. Can J Microbiol 32:610–613
    [Google Scholar]
  10. Van Elsas J.D., Govaert J.M., Van Veen J.A. 1987; Transfer of plasmid pFT30 between bacilli in soil as influenced by bacterial population dynamics and soil conditions.. Soil Biol Biochem 19:639–647
    [Google Scholar]
  11. Wimpenny J., Waters P. 1987; The use of gel-stabilized gradient plates to map the responses of microorganisms to three or four environmental factors varied simultaneously.. FEMS Microbiol Lett 40:263–267
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-141-10-2713
Loading
/content/journal/micro/10.1099/13500872-141-10-2713
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error