1887

Abstract

Summary: The adherence of to extracellular matrix proteins may be a critical step in the pathogenesis of candidiasis. Yeast cell adherence to type I and IV collagen, fibronectin and laminin was blocked by peptide fragments from denatured type I collagen (gelatin). Gelatin fragments were obtained by digestion of the reduced protein with trypsin or CNBr. The fragments did not have antifungal properties, presumably inhibiting adherence by blocking receptors (adhesins) on the surface of the fungus. A 10-mer (GQRGVVGLPG) fashioned from the a-1 chain of type I collagen reduced adherence by 68%. However, a gelatin peptide possessing 47 amino acids reduced fungal adherence to type I collagen by 100%. Peptides derived from the biocompatible protein gelatin, therefore, may have a potential role in reducing the adherence of the fungus to host proteins.

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/content/journal/micro/10.1099/13500872-141-10-2681
1995-10-01
2021-10-25
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References

  1. Adelmann-Grill B.C. 1981; Independence of reciprocal attachment sites on denatured collagen and fibronectin from antigenic sites, and binding of gelatin-based plasma substitutes to plasma fibronectin.. Collagen Relat Res 1:367–375
    [Google Scholar]
  2. Calderone R.A., Braun P.C. 1991; Adherence and receptor relationships of Candida albicans.. Microbiol Rev 55:1–20
    [Google Scholar]
  3. Klotz S.A. 1990; Adherence of Candida albicans to components of the subendothelial extracellular matrix.. FEMS Microbiol Lett 68:249–254
    [Google Scholar]
  4. Klotz S.A. 1992; Fungal adherence to the vascular compartment: a critical step in the pathogenesis of disseminated candidiasis.. Clin Infect Dis 14:340–347
    [Google Scholar]
  5. Klotz S.A., Smith R.L. 1991; A fibronectin receptor on Candida albicans mediates adherence of the fungus to extracellular matrix.. J Infect Dis 163:604–610
    [Google Scholar]
  6. Klotz S.A., Smith R.L., Stewart B.W. 1992; Effect of an arginine-glycine-aspartic acid-containing peptide on hematogenous candidal infections in rabbits.. Antimicrob Agents Chemother 36:132–136
    [Google Scholar]
  7. Penn C., Klotz S.A. 1994; Binding of plasma fibronectin to Candida albicans occurs through the cell binding domain.. Microb Pathog 17:387–393
    [Google Scholar]
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