1887

Abstract

Summary: A gene encoding the chitin synthase of was cloned. DNA sequencing and comparison with another gene described elsewhere indicated that the gene encoded a polypeptide with 775 amino acid residues, a protein with one less amino acid than that encoded by the gene. A six-base alteration was observed between the two genes, suggesting that the gene is a variant gene of the . The pH profile/activity relationship of canChs1A in permeabilized cells was identical to that of canChs1. The canChs1A enzyme was competitively inhibited by polyoxin D (5.2 μM) and nikkomycin Z (12 μM). When the cloned gene was expressed in a mutant that exhibited aberrant morphology, the normal structure was restored. We conclude that the function of the gene is similar to that of in

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1995-10-01
2024-04-19
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