1887

Abstract

Summary: A gene encoding the chitin synthase of was cloned. DNA sequencing and comparison with another gene described elsewhere indicated that the gene encoded a polypeptide with 775 amino acid residues, a protein with one less amino acid than that encoded by the gene. A six-base alteration was observed between the two genes, suggesting that the gene is a variant gene of the . The pH profile/activity relationship of canChs1A in permeabilized cells was identical to that of canChs1. The canChs1A enzyme was competitively inhibited by polyoxin D (5.2 μM) and nikkomycin Z (12 μM). When the cloned gene was expressed in a mutant that exhibited aberrant morphology, the normal structure was restored. We conclude that the function of the gene is similar to that of in

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-141-10-2673
1995-10-01
2021-10-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/141/10/mic-141-10-2673.html?itemId=/content/journal/micro/10.1099/13500872-141-10-2673&mimeType=html&fmt=ahah

References

  1. Au-Young J., Robbins P.W. 1990; Isolation of a chitin synthase gene (CHS1) from Candida albicans by expression in Saccharomyces cerevisiae.. Mol Microbiol 4:197–207
    [Google Scholar]
  2. Becker D.M., Guarente L. 1990; High efficiency transformation of yeast by electroporation.. Methods Enzymol 194:182–186
    [Google Scholar]
  3. Beth Din A.B., Yarden O. 1994; The Neurospora crassa chs-2 gene encodes a non-essential chitin synthase.. Microbiology 140:2189–2197
    [Google Scholar]
  4. Bowen A.R., Chen-Wu J.L., Momany M., Young R., Szaniszlo P.J., Robbins P.W. 1992; Classification of fungal chitin synthases.. Proc Natl Acad Sci USA 89:519–523
    [Google Scholar]
  5. Bulawa C.E. 1992; CSD2, CSD3, and CSD4, genes required for chitin synthesis in Saccharomyces cerevisiae: the CSD2 gene product is related to chitin synthase and to developmentally regulated proteins in Rhizobium species and Xenopus laevis.. Mol Cell Biol 12:1764–1776
    [Google Scholar]
  6. Bulawa C.E., Osmond B.C. 1990; Chitin synthase I and chitin synthase II are not required for chitin synthesis in vivo in Saccharomyces cerevisiae.. Proc Natl Acad Sci USA 87:7424–7428
    [Google Scholar]
  7. Bulawa C.E., Slater M., Cabib E., Au-Young J., Sburlati A., Adair W.L. Jr Robbins P.W. 1986; The S. cerevisiae structural gene for chitin synthase is not required for chitin synthase in vivo.. Cell 46:213–225
    [Google Scholar]
  8. Cabib E. 1991; Differential inhibition of chitin synthases 1 and 2 from Saccharomyces cerevisiae by polyoxin D and nikkomycins.. Antimicrob Agents Chemother 35:170–173
    [Google Scholar]
  9. Cabib E., Sburlati A., Bowers B., Silverman S.J. 1989; Chitin synthase 1, an auxiliary enzyme for chitin synthesis in Saccharomyces cerevisiae.. J Cell Biol 108:1665–1672
    [Google Scholar]
  10. Chattaway F.W., Holmes M.R., Barlow A.J.E. 1968; Cell wall composition of the mycelial and blastospore forms of Candida albicans.. J Gen Microbiol 51:367–376
    [Google Scholar]
  11. Chen-Wu J.L., Zwicker J., Bowen A.R., Robbins P.W. 1992; Expression of chitin synthase genes during yeast and hyphal growth phases of Candida albicans.. Mol Microbiol 6:497–502
    [Google Scholar]
  12. Delome E. 1989; Transformation of Saccharomyces cerevisiae by electroporation.. Appl Environ Microbiol 55:2242–2246
    [Google Scholar]
  13. Fernandez M.P., Correa J.U., Cabib E. 1982; Activation of chitin synthase in permeabilized cells of a Saccharomyces cerevisiae mutant lacking proteinase B.. J Bacteriol 152:1255–1264
    [Google Scholar]
  14. Fukuda R., Horiuchi H., Ohta A., Takagi M. 1994; The prosequence of Rhizopus niveus aspartic proteinase-I supports correct folding and secretion of its mature part in Saccharomyces cerevisiae.. J Biol Chem 269:9556–9561
    [Google Scholar]
  15. Gow N.A.R. 1994; Growth and guidance of the fungal hypha.. Microbiology 140:3193–3205
    [Google Scholar]
  16. Motoyama T., Sudoh M., Horiuchi H., Ohta A., Takagei M. 1994; Isolation and characterization of two chitin synthase genes of Rhiyopus oligosporus.. Biosci Biotech Biochem 58:1685–1693
    [Google Scholar]
  17. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 74:5463–5467
    [Google Scholar]
  18. Sburlati A., Cabib E. 1986; Chitin synthase 2, a presumptive participant in septum formation in S. cerevisiae. . J Biol Chem 261:15147–15152
    [Google Scholar]
  19. Shaw J., Mol P.C., Bowers B., Silverman S.J., Valdivieso M.H., Duran A., Cabib E. 1991; The function of chitin synthase 2 and 3 in the Saccharomyces cerevisiae cell cycle.. J Cell Biol 114:111–123
    [Google Scholar]
  20. Silverman S.J. 1989; Similar and different domains of chitin synthases 1 and 2 of S. cerevisiae: two isozymes with distinct functions.. Yeast 5:459–467
    [Google Scholar]
  21. Silverman S.J., Sburlati A., Slater M.L., Cabib E. 1988; Chitin synthase 2 is essential for septum formation and cell division in Saccharomyces cerevisiae.. Proc Natl Acad Sci USA 85:4735–4739
    [Google Scholar]
  22. Soli D.R. 1992; High-frequency switching in Candida albicans.. Clin Microbiol Rev 5:183–203
    [Google Scholar]
  23. Sudoh M., Nagahashi S., Doi M., Ohta A., Takagi M., Arisawa M. 1993; Cloning of the chitin synthase 3 gene from Candida albicans and its expression during yeast-hyphal transition.. Mol & Gen Genet 241:351–358
    [Google Scholar]
  24. Sullivan P.A., Yin C.Y., Molloy C., Templeton M.D. 1983; An analysis of the metabolism and cell wall composition ofCandida albicans during germ tube formation.. Can J Microbiol 29:1514–1525
    [Google Scholar]
  25. Valdivieso M.H., Mol P.C., Shaw J.A., Cabib E., Duran A. 1991; CALI, a gene required for activity of chitin synthase 3 in Saccharomjces cerevisiae. . J Cell Biol 114:101–109
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-141-10-2673
Loading
/content/journal/micro/10.1099/13500872-141-10-2673
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error