1887

Abstract

Summary: The addition of glucose to derepressed cells of provokes a cAMP signal and activation of the cytoplasmic neutral trehalase. This transduction pathway does not require functional RAS protein since -disrupted cells exhibited a glucose response similar to that shown by control cells. Treatment of activated trehalase by alkaline phosphatase resulted in enzyme deactivation suggesting that trehalase may be modulated by reversible phosphorylation through cAMP-dependent protein kinase (PKA1). However, the addition of glucose to derepressed growing cells of lacking the catalytic subunit of protein kinase A (? strains) induced stimulation of trehalase as well as phosphorylation of the enzyme protein. This glucose-induced response was absent in -deficient cells from resting cultures. Addition of exogenous cAMP activated trehalase in normal growing cells but failed to produce any effect on trehalase in -disrupted growing cells. These results confirm the occurrence of a PKA1-dependent pathway for trehalase activation and imply the existence of another glucose-induced phosphorylation pathway capable of activating trehalase during growth by a distinct, cAMP-independent protein kinase. At least one of the upstream components playing a role in the transduction of this alternative signal is either absent or inactive in cells from stationary phase and sporulated cultures. Cells harbouring the disrupted gene responded also to a heat-shock signal by increasing trehalase activity, thus revealing that this enzyme may be a target common to various signalling pathways in the fission yeast.

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1995-10-01
2021-05-12
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References

  1. Beach D., Rodgers L., Gould J. 1985; RAN1+ controls the transition from mitotic division to meiosis in fission yeast.. Curr Genet 10:297–311
    [Google Scholar]
  2. Berridge M.J. 1987; Inositol trisphosphate and diacylglycerol: two interacting second messengers.. Annu Rev Biochem 56:159–163
    [Google Scholar]
  3. Brandao R.L., Magalhaes-Rocha N.M., Alijo B., Ramos J., Thevelein J.M. 1994; Possible involvement of a phosphatidylinositol-type signaling pathway in glucose-induced activation of plasma membrane H+-ATPase and cellular proton extrusion in the yeast Saccharomyces cerevtsiae.. Biochim Biophys Acta 1223:117–124
    [Google Scholar]
  4. Carrillo D., Vicente-Soler J., Gacto M. 1992; Activation of neutral trehalase by fermentable sugars and cAMP in the fission yeast Schiyosaccharomyces pombe.. FEMS Microbiol Lett 98:61–66
    [Google Scholar]
  5. Carrillo D., Vicente-Soler J., Gacto M. 1994; Cyclic AMP signalling pathway and trehalase activation in the fission yeast Schiɀosaccharomyces pombe.. Microbiology 140:1467–1472
    [Google Scholar]
  6. De Virgilio C., Simmen U., Hottiger T., Boiler T., Wiemken A. 1990; Heat shock induces enzymes of trehalose metabolism, trehalose accumulation and thermotolerance in Schiɀosaccharomyces pombe, even in the presence of cycloheximide.. FEBS Lett 273:107–110
    [Google Scholar]
  7. De Virgilio C., Muller J., Boller T., Wiemken A. 1991; A constitutive, heat shock-activated neutral trehalase occurs in Schiɀosaccharomycespombe in addition to the sporulation-specific acid trehalase.. FEMS Microbiol Lett 84:84–90
    [Google Scholar]
  8. De Voti J., Seydoux G., Beach D., McLeod M. 1991; Interaction between ran1+ protein kinase and cAMP dependent protein kinase as negative regulators of fission yeast meiosis.. EMBO J 10:3759–3768
    [Google Scholar]
  9. Fukui Y., Kozasa T., Kajiro Y., Takeda T., Yamamoto M. 1986; Role of a ras homologue in the life cycle of Schiɀosaccharomyces pombe.. Cell 44:329–336
    [Google Scholar]
  10. Fukui Y., Miyake S., Satoh M., Yamamoto M. 1989; Characterization of the Schiɀosaccharomyces pombe ral2 gene implicated in activation of the ras1 gene product.. Mol Cell Biol 9:5617–5622
    [Google Scholar]
  11. Herskowitz I. 1995; MAP kinase pathways in yeast: for mating and more.. Cell 80:187–197
    [Google Scholar]
  12. Hoffman C.S., Winston F. 1991; Glucose repression of transcription of the Schiɀosaccharomyces pombe fbp 1 gene occurs by a cAMP signaling pathway.. Genes & Dev 5:561–571
    [Google Scholar]
  13. Leupold U. 1950; Die Vererbung von Homothallie und Heterothallie bei Schiɀosaccharomyces pombe.. C R Trav Lab Carlsberg Ser Physiol 24:381–480
    [Google Scholar]
  14. Maeda T., Watanabe Y., Kunitomo H., Yamamoto M. 1994; Cloning of the pka1 gene encoding the catalytic subunit of the cAMP-dependent protein kinase in Schiɀosaccharomyces pombe.. J Biol Chem 269:9632–9637
    [Google Scholar]
  15. McLeod M., Beach D. 1986; Homology between the ran1+ gene of fission yeast and protein kinases.. EMBO J 5:3665–3671
    [Google Scholar]
  16. Mochizuki N., Yamamoto M. 1992; Reduction of the intracellular cAMP level triggers initiation of sexual development in fission yeast.. Mol & Gen Genet 233:17–24
    [Google Scholar]
  17. Nielsen O., Davey J., Egel R. 1992; The ras1 function of Schiɀosaccharomyces pombe mediates pheromone-induced transcription.. EMBO J 11:1391–1395
    [Google Scholar]
  18. Shimma Y., Uno I. 1990; Isolation and characterization of neomycin-sensitive mutants in Saccharomyces cerevisiae.. J Gen Microbiol 136:1753–1761
    [Google Scholar]
  19. Tamakori T., Nomoto H., Takahashi I., Kato Y., Monimoto M., Tomita F. 1986; Staurosporine, a potent inhibitor of phospholipid/Ca++ dependent protein kinase.. Biochem Biophys Res Commun 135:397–402
    [Google Scholar]
  20. Thevelein J.M. 1991; Fermentable sugars and intracellular acidification as specific activators of the RAS-adenylate cyclase signalling pathway in yeast: the relationship to nutrient-induced cell cycle control.. Mol Microbiol 5:1301–1307
    [Google Scholar]
  21. Van Laere A. 1989; Trehalose, reserve and/or stress metabolite?. FEMS Microbiol Rev 63:201–210
    [Google Scholar]
  22. Yu G., Li J., Young D. 1994; The Schiɀosaccharomyces pombe pka 1gene, encoding a homolog of cAMP-dependent protein kinase.. Gene 151:215–220
    [Google Scholar]
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