A plasmid encoding enzymes for nylon oligomer degradation: nucleotide sequence and analysis of pOAD2 Free

Abstract

Summary: The entire nucleotide sequence of nylon oligomer degradative plasmid pOAD2 from sp. KI723T1 was determined. pOAD2 comprises 45519 bp, with a 66.6 mol% G + C content. The precise loci of the four nylon oligomer degradation genes, namely (6-aminohexanoate-cyclic-dimer hydrolase gene), ) (6-aminohexanoate-dimer hydrolase), ' (a gene having 88% homology to ) and (endo-type 6-aminohexanoate oligomer hydrolase), and five IS6100 elements were identified on this plasmid. Comparison of the sequence of pOAD2 with those in the GenBank and EMBL databases revealed that the deduced amino acid sequences from eight regions of pOAD2 had significant similarity with the sequences of gene products such as (oligopeptide permeases), (filamentation temperature sensitive), (isopenicillin ) and (plasmid incompatibility). A functional map of pOAD2 is presented.

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1995-10-01
2024-03-28
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References

  1. Alloing G., Trombe M.-C., Claverys J.-P. 1990; The ami locus of the gram-positive bacterium Streptococcus pneumoniae is similar to binding protein-dependent transport operons of gram-negative bacteria.. Mol Microbiol 4:633–644
    [Google Scholar]
  2. Alvarez E., Meesschaert B., Montenegro E., Gutierrez S., Diez B., Barredo J.L., Martin J.F. 1993; The isopenicillin-N acyltransferase and isopenicillin-N amidohydrolase, 6-amino- penicillanic acid acyltransferase and penicillin amidase activities, all of which are encoded by the single penDE gene.. Eur J Biochem 215:323–332
    [Google Scholar]
  3. Birnboim H.C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA.. Nucleic Acids Res 7:1513–1523
    [Google Scholar]
  4. Bolivar F., Rodriguez R.L., Greene P.J., Betlach M.C., Heyneker H.L, Boyer H.W., Crosa J.H., Falkow S. 1977; Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.. Gene 2:95–113
    [Google Scholar]
  5. Davis M.A., Martin K.A., Austin S.J. 1992; Biochemical activities of the parA partition protein of the P1 plasmid.. Mol Microbiol 6:1141–1147
    [Google Scholar]
  6. Gill D.R., Hatfull G.F., Salmond G.P. 1986; A new cell division operon in Escherichia coli. . Mol & Gen Genet 205:134–145
    [Google Scholar]
  7. Hiles I.D., Gallagher M.P., Jamieson D.J., Higgins C.F. 1987; Molecular characterization of the oligopeptide permease of Salmonella typbimurium. . J Mol Biol 195:125–142
    [Google Scholar]
  8. Kanagawa K., Negoro S., Takada N., Okada H. 1989; Plasmid dependence of Pseudomonas sp. strain NK87 enzymes that degrade 6- aminohexanoate-cyclic dimer.. J Bacteriol 171:3181–3186
    [Google Scholar]
  9. Kanagawa K., Oishi M., Negoro S., Urabe I., Okada H. 1993; Characterization of the 6-aminohexanoate-dimer hydrolase from Pseudomonas sp. NK87.. J Gen Microbiol 139:787–795
    [Google Scholar]
  10. Kato K., Ohtsuki K., Mitsuda H., Yomo T., Negoro S., Urabe I. 1994; Insertion sequence 1S6100 on plasmid pOAD2, which degrades nylon oligomers.. J Bacteriol 176:1197–1200
    [Google Scholar]
  11. Kinoshita S., Negoro S., Muramatsu M., Bisaria V.S., Sawada S., Okada H. 1977; 6-Aminohexanoic acid cyclic dimer hydrolase: a new cyclic amide hydrolase produced by Acbromobacter guttatus KI72.. Eur J Biochem 80:489–495
    [Google Scholar]
  12. Kinoshita S., Terada T., Taniguchi T., Takene Y., Masuda S., Matsunaga N., Okada H. 1981; Purification and characterization of 6-aminohexanoic acid oligomer hydrolase of Flavo-bacterium sp. KI72.. Eur J Biochem 116:547–551
    [Google Scholar]
  13. Mori H., Kondo A., Ohshima A., Ogura T., Hiraga S. 1986; Structure and function of the F plasmid genes essential for partitioning.. J Mol Biol 192:1–15
    [Google Scholar]
  14. Negoro S., Shinagawa H., Nakata A., Kinoshita S., Hatozaki T., Okada H. 1980; Plasmid control of 6-aminohexanoic acid cyclic dimer degradation enzymes of Flavobacterium sp. KI72.. J Bacterial 143:238–245
    [Google Scholar]
  15. Negoro S., Taniguchi T., Kanaoka M., Kimura H., Okada H. 1983; Plasmid-determined enzymatic degradation of nylon oligomers.. J Bacteriol 155:22–31
    [Google Scholar]
  16. Negoro S., Kakudo S., Urabe I., Okada H. 1992; A new nylon oligomer degradation gene (nylC) on plasmid pOAD2 of Flavo-bacterium sp.. J Bacteriol 174:7948–7953
    [Google Scholar]
  17. Okada H., Negoro S., Kimura H., Nakamura S. 1983; Evolutionary adaptation of plasmid-encoded enzymes for degrading nylon oligomers.. Nature 306:203–206
    [Google Scholar]
  18. Okazaki M., Uemura T., Nishimori Y., Wakamatsu K., Shimosaka M., Saito H., Negoro S., Okada H. 1988; Analysis of a promoter-like region in Flavobacterium which controls 6-aminohexanoic acid linear oligomer hydrolase gene expression in Escherichia coli. . J Ferment Technol 66:489–494
    [Google Scholar]
  19. Pearson W.R., Lipman D.J. 1988; Improved tools for biological sequence analysis.. Proc Natl Acad Sci USA 852444–2448
    [Google Scholar]
  20. Perego M., Higgins C.F., Pearce S.R., Gallagher M.P., Hoch J.A. 1991; The oligopeptide transport system of Bacillus subtilisplays a role in the initiation of sporulation.. Mol Microbiol 5:173–185
    [Google Scholar]
  21. Rudner D.Z., LeDeaux J.R., Ireton K., Grossman A.D. 1991; The spoOK locus of Bacillus subtilis is homologous to the oligopeptide permease locus and is required for sporulation and competence.. J Bacteriol 173:1388–1398
    [Google Scholar]
  22. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Eaboratory Manual, 2nd. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  23. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 745463–5467
    [Google Scholar]
  24. Tabata S., Hooykaas P.J., Oka A. 1989; Sequence determination and characterization of the replicator region in the tumor-inducing plasmid pTiB6S3.. J Bacteriol 171:1665–1672
    [Google Scholar]
  25. Thomas C.M., Smith C.A. 1986; The trfB region of broad host range plasmid RK2: the nucleotide sequence reveals incC and key regulatory gene trfB/korA/korD as overlapping genes.. Nucleic Acids Res 14:4453–4469
    [Google Scholar]
  26. Tobin M.B., Fleming M.D., Skatrud P.L., Miller J.R. 1990; Molecular characterization of the acyl-coenzyme A: isopenicillin-N acyltransferase gene (penDE) from Penicillium chrysogenum and Aspergillus nidulans and activity of recombinant enzyme in Escherichia coli. . J Bacteriol 172:5908–5914
    [Google Scholar]
  27. Tsuchiya K., Fukuyama S., Kanzaki N., Kanagawa K., Negoro S., Okada H. 1989; High homology between 6-aminohexanoate-cyclic-dimer hydrolases of Flavobacterium and Pseudomonas strains.. J Bacteriol 171:3187–3191
    [Google Scholar]
  28. Vogelstein B., Gillespie D. 1979; Preparative and analytical purification of DNA from agarose.. Proc Natl Acad Sci USA 76615–619
    [Google Scholar]
  29. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors.. Gene 33:103–119
    [Google Scholar]
  30. Yomo T., Urabe I., Okada H. 1992; No stop codons in the antisense strands of the genes for nylon oligomer degradation.. Proc Natl Acad Sci USA 893780–3784
    [Google Scholar]
  31. Zhou Z.E., Nishimori Y., Shimosaka M., Okazaki M. 1990; Transcription of a gene for 6-aminohexanoic acid linear oligomer hydrolase of Flavobacterium sp. KI72 in Escherichia coli. . J Ferment Bioeng 70:266–268
    [Google Scholar]
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