%0 Journal Article %A Deeble, V. Jayne %A Lindley, Helen K. %A Fazeli, Mohammad Reza %A Cove, Jonathan H. %A Baumberg, Simon %T Depression of streptomycin production by Streptomyces griseus at elevated growth temperature: studies using gene fusions %D 1995 %J Microbiology, %V 141 %N 10 %P 2511-2518 %@ 1465-2080 %R https://doi.org/10.1099/13500872-141-10-2511 %K strepromycin %K Streptomyces %K regulation of secondary metabolism %K antibiotics %K antibiotic production %I Microbiology Society, %X Summary: Streptomyces griseus ATCC 12475 fails to produce streptomycin when grown at 34 °C or above, although growth is appreciable up to at least 37 °C. This depression of streptomycin production at elevated growth temperature is manifest equally in liquid and on solid, and with complex and minimal, media. We report studies with gene fusions of the reporter genes aph or xylE to restriction fragments containing the streptomycin biosynthesis promoter PstrB1. aph constructs were in high, and xylE constructs in low, copy number vectors. Two strB1 promoter fragments were used, one requiring activation by the pathway-specific activator StrR of S. griseus, the other reportedly activator independent. PstrB1 expression in the aph constructs in S. griseus and in S. lividans was significantly reduced at 37 °C compared to 30 °C. Some of this reduction could be explained by lower plasmid copy number at the higher temperature, but strR-dependent expression was clearly temperature controlled. Using the xylE reporter system, the temperature dependence of PstrB1 expression was confirmed but, surprisingly, the strR dependence of the two promoter fragments differed from that observed in the multicopy aph constructs. These data identify a temperature-dependent promoter which may contribute to the depressive effect of elevated growth temperature on streptomycin production. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-141-10-2511