RT Journal Article SR Electronic(1) A1 Suizu, Tetsuyoshi A1 Tsutsumi, Hiroko A1 Kawado, Akitsugu A1 Inose, Tomoko A1 Suginami, Koji A1 Murata, KousakuYR 1995 T1 Analysis of lysine-dependent yeast sporulation: a decrease in cyclic AMP is not required for initiation of meiosis and sporulation in Saccharomyces cerevisiae JF Microbiology, VO 141 IS 10 SP 2463 OP 2469 DO https://doi.org/10.1099/13500872-141-10-2463 PB Microbiology Society, SN 1465-2080, AB Summary: Cells of the yeast Saccharomyces cerevisiae sporulated in nutrient-rich medium containing l-lysine. Sporulation was specific to the presence of L-lysine and was initiated when the cellular content of this basic amino acid reached approximately 0·2-0·5 mmol (g cells)−1, at early stationary phase. The formation of asci was most efficient at pH 7·0 and 50-100 mM L-lysine; in these optimum conditions, the sporulation frequency reached about 60% after 5 d incubation. The L-lysine-dependent sporulation system in nutrient-rich conditions was distinct from the currently used potassium-acetate-dependent system in nutrient-deficient conditions. Analysis of the L-lysine-dependent system indicated that, prior to entrance into meiosis and/or sporulation processes, the yeast cells change in shape, their pool sizes for L-cysteine and glutathione alter, and they synthesize a protein with a molecular mass of 15 kDa. A low level of cAMP was not required for the entrance into meiosis and/or sporulation., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-141-10-2463