1887

Abstract

Summary: Cells of the yeast sporulated in nutrient-rich medium containing -lysine. Sporulation was specific to the presence of L-lysine and was initiated when the cellular content of this basic amino acid reached approximately 0·2-0·5 mmol (g cells), at early stationary phase. The formation of asci was most efficient at pH 7·0 and 50-100 mM L-lysine; in these optimum conditions, the sporulation frequency reached about 60% after 5 d incubation. The L-lysine-dependent sporulation system in nutrient-rich conditions was distinct from the currently used potassium-acetate-dependent system in nutrient-deficient conditions. Analysis of the L-lysine-dependent system indicated that, prior to entrance into meiosis and/or sporulation processes, the yeast cells change in shape, their pool sizes for L-cysteine and glutathione alter, and they synthesize a protein with a molecular mass of 15 kDa. A low level of cAMP was not required for the entrance into meiosis and/or sporulation.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-141-10-2463
1995-10-01
2021-07-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/141/10/mic-141-10-2463.html?itemId=/content/journal/micro/10.1099/13500872-141-10-2463&mimeType=html&fmt=ahah

References

  1. Cameron S., Levin L., Zoller M., Wigler M. 1988; cAMP- independent control of sporulation, glycogen metabolism, and heat shock resistance in S. cerevisiae. . Cell 53:555–566
    [Google Scholar]
  2. Freese E.B., Chu M.I., Freese E. 1982; Initiation of yeast sporulation by partial carbon, nitrogen, or phosphate deprivation.. J Bacteriol 149:840–851
    [Google Scholar]
  3. Freese E.B., Olempska-Beer Z., Hartig A., Freese E. 1984; Initiation of meiosis and sporulation of Saccharomyces cerevisiae by sulfur or guanine deprivation.. Dev Biol 102:438–451
    [Google Scholar]
  4. Hoshino T., Mizutani A., Hidaka H., Yamane T. 1992; Identification of Ca2+/calmodulin-dependent protein kinase and endogenous substrate of Fusarium oxysporum. . FEMS Microbiol Lett 94:27–30
    [Google Scholar]
  5. Kano K., Murata K., Kimura A., Aiba H. 1983; Cloning and amplification of the adenylate cyclase gene.. Agric Biol Chem 47:1079–1085
    [Google Scholar]
  6. Kawado A., Suizu T., Imayasu S., Kimura A., Murata K. 1993; Yeast sporulation induced by lysine during growth in nutrition-rich conditions.. J Ferment Bioeng 76:391–394
    [Google Scholar]
  7. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature 227:680–685
    [Google Scholar]
  8. Matsumoto K., Uno I., Oshima Y., Ishikawa T. 1982; Isolation and characterization of yeast mutants deficient in adenylate cyclase and cAMP-dependent protein kinase.. Proc Natl Acad Sci USA 792355–2359
    [Google Scholar]
  9. Matsumoto K., Uno I., Ishikawa T. 1983; Initiation of meiosis in yeast mutants defective in adenylate cyclase and cyclic AMP-dependent protein kinase.. Cell 32:417–423
    [Google Scholar]
  10. Matsumoto K., Uno I., Ishikawa T. 1984; Identification of the structural gene and nonsense alleles for adenylate cyclase in Saccharomyces cerevisiae. . J Bacteriol 157:277–282
    [Google Scholar]
  11. Matsumoto K., Uno I., Ishikawa T. 1985; Genetic analysis of the role of cAMP in yeast.. Yeast 1:15–24
    [Google Scholar]
  12. Olempska-Beer Z., Freese E. 1987; Initiation of meiosis and sporulation in Saccharomyces cerevisiae does not require a decrease in cyclic AMP.. Mol Cell Biol 7:2141–2147
    [Google Scholar]
  13. Suizu T., Tsutsumi H., Ohtake Y., Kawado A., Imayasu S., Kimura A. 1994a; Absolute glutathione requirement for sporulation of a yeast Saccharomyces cerevisiae. . Biochem Biophys Res Commun 205:1151–1155
    [Google Scholar]
  14. Suizu T., Tsutsumi H., Kawado A., Murata K., Imayasu S. 1994b; On the importance of calcium and magnesium ions in yeast sporulation.. J Ferment Bioeng 77:275–277
    [Google Scholar]
  15. Tietze F. 1969; Enzymatic method for quantitative determination of nanogram amounts of total and oxidized glutathione.. Anal Biochem 27:502–522
    [Google Scholar]
  16. Yamashita I. 1994; Pheromone that induces meiosis in Saccharomyces cerevisiae. . Nippon Nogeikagaku Kaishi (Journal of the Agricultural Chemical Society of Japan) 68:100 (in Japanese).
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-141-10-2463
Loading
/content/journal/micro/10.1099/13500872-141-10-2463
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error