RT Journal Article SR Electronic(1) A1 Mrkeberg, Rikke A1 Carlsen, Morten A1 Nielsen, JensYR 1995 T1 Induction and repression of a-amylase production in batch and continuous cultures of Aspergillus oryzae JF Microbiology, VO 141 IS 10 SP 2449 OP 2454 DO https://doi.org/10.1099/13500872-141-10-2449 PB Microbiology Society, SN 1465-2080, AB Summary: The intra- and extracellular concentrations of a-amylase in Aspergillus oryzae have been measured during batch culture of a wild-type strain and two recombinant strains. The mean intracellular level for the two recombinant strains was about four to five times the level of the wild-type strain. The recombinant strains also had a higher a-amylase productivity, whereas the residence time of the intracellular a-amylase pool was approximately the same for the three strains. At high glucose concentrations there was a low constitutive synthesis of a-amylase, whereas at low glucose concentrations derepression resulted in an increased production rate. Shifts from a glucose- to a maltose-limited chemostat showed that maltose induces both the production and secretion of a-amylase. Finally, from immunoblots, both a glycosylated and an unglycosylated a-amylase have been detected., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-141-10-2449