1887

Abstract

Summary: A physical map of ATCC 43954 was constructed from DNA fragments generated by I (5′ G/TCGAC), I (5′ GG/CGCC) and HII (5′ G/CGCGC) restriction digests separated using pulsed-field gel electrophoresis. The size of the genome was approximately 2000 kb, providing evidence of the largest genome sized to date. Twenty-one fragments created from these restriction digests were assembled into a physical map using a combination of complementary methods including cross-Southern hybridization, hybridization fingerprint analysis and hybridization with homologous and heterologous (from ) gene probes. The position of ten genetic loci, including that of the iron-uptake regulatory () gene, were localized to the physical map. A genomic library of ATCC 43954 was constructed in lambda Gem-11 vector. Fifty thousand recombinants with an average size of 16 kb represent a library about 200 times the size of the genome. Using DNA probes, clones representing and genes were isolated and localized to the physical map.

Keyword(s): Campylobacter and genomic map
Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-141-10-2417
1995-10-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/141/10/mic-141-10-2417.html?itemId=/content/journal/micro/10.1099/13500872-141-10-2417&mimeType=html&fmt=ahah

References

  1. Allardet-Servent A, Carles-Nurit M.J, Bourg G., Michaux S., Ramuz M. 1991; Physical map of the brucella melitensis 16M chromosome.. J Bacterial 173:2219–2224
    [Google Scholar]
  2. Alleman A.R., Kamper S.M., Viseshakul N., Barbet A.F. 1993; Analysis of the Anaplasma marginale genome by pulsed-field electrophoresis.. J Gen Microbiol 139:2439–2444
    [Google Scholar]
  3. Chan V.L., Bingham H.L. 1992; Lysyl-tRNA synthetase gene of Campylobacter jejuni. . J Bacterial 174:695–701
    [Google Scholar]
  4. Chang N., Taylor D.E. 1990; Use of pulsed-field agarose gel electrophoresis to size genomes of Campylobacter species and to construct a SalI map of Campylobacter jejuni UA580.. J bacterial 172:5211–5217
    [Google Scholar]
  5. Chusid M.J., Wortmann D.W., Dunne W.M. 1990; 'Campylobacter upsaliensis'sepsis in a boy with acquired hypogammaglobulinemia.. Diagn Microbiol Infect Dis 13:367–369
    [Google Scholar]
  6. Goossens H., Vlaes L., DeBoeck M., Pot B., Kersters K., De Mol P., Butzler J.P., Van Damme P. 1990; Is 'Campylobacter upsaliensis' an unrecognised cause of human disease?. lancet 335:584–586
    [Google Scholar]
  7. Grossberger D. 1987; Minipreps of DNA from bacteriophage lambda.. Nucleic Acids Res 15:6737
    [Google Scholar]
  8. Honeycutt R.J., McClelland M., Sobral B.W.S. 1993; Physical map of the genome of Rhizobium meliloti 1021.. J Bacteriol 175:6945–6952
    [Google Scholar]
  9. Hong Y., Wong T., Bourke B., Chan V.L. 1995; An isoleucyl- tRNA synthetase gene from Campylobacter jejuni. . Microbiology 141:2561–2567
    [Google Scholar]
  10. Khawaja R., Neote K., Bingham H.L., Penner J.L., Chan V.L. 1992; Cloning and sequence analysis of the flagellin gene of Campylobacter jejuni TGH9011.. Curr Microbiol 24:213–221
    [Google Scholar]
  11. Kim N.W., Bingham H., Khawaja R., Louie H., Hani E., Neote K., Chan V.L. 1992; Physical map of Campylobacter jejuniTGH9011 and localization of 10 genetic markers by use of pulsed- field gel electrophoresis.. J Bacteriol 174:3494–3498
    [Google Scholar]
  12. Kim N.W., Lombardi R., Bingham H., Hani E., Louie H., Ng D., Chan V.L. 1993; Fine mapping of the three rRNA operons on the updated genomic map of Campylobacter jejuni TGH9011 (ATCC 43431). . J Bacteriol 175:7468–7470
    [Google Scholar]
  13. Ladefoged S.A., Christiansen G. 1992; Physical and genetic mapping of the genomes of five Mycoplasma hominis strains by pulsed-field gel electrophoresis.. J Bacteriol 174:2199–2207
    [Google Scholar]
  14. Lastovica A.J., Le Roux E., Penner J.L. 1989; 'Campylobacter upsaliensis' isolated from blood cultures of pediatric patients.. J Clin Microbiol 27:657–659
    [Google Scholar]
  15. Lee J.J., Smith H.O. 1988; Sizing of the Haemophilus influenizaeRd genome by pulsed-field agarose gel electrophoresis.. J Bacteriol 170:4402–4405
    [Google Scholar]
  16. Lee J.J., Smith H.O., Redfield R.J. 1989; Organization of the Haemophilus Rd genome.. J Bacteriol 171:3016–3024
    [Google Scholar]
  17. Maslow J.N., Slutsky A.M., Arbeit R.D. 1993; Application of pulsed-field gel electrophoresis to molecular epidemiology.. In Diagnostic Molecular Microbiology: Principles and Applications, pp. 563–572 Persing D. H., Smith T. F., Tenover F. C., White. T. J. Edited by Washington, DC:: American Society for Microbiology.;
    [Google Scholar]
  18. Megraud F.8EBonnet. 1986; Unusual camplyobacters in human feces.. J Infect 12:275–276
    [Google Scholar]
  19. Murray B.E., Singh K.V., Ross R.P., Heath J.D., Dunny G.M., Weinstock G.M. 1993; Generation of restriction map of Enterococcus faecalis OGl and investigation of growth requirements and regions encoding biosynthetic function.. J Bacteriol 175:5216–5223
    [Google Scholar]
  20. Neumann B., Pospiech A., Schairer H.U. 1993; A physical and genetic map of the Stigmatella aurantiaca DW4/3.1 chromosome.. Mol Microbiol 10:1087–1099
    [Google Scholar]
  21. Nuijten P. J. M., Bartels C., Bleumink-Pluym N. M. C., Gaastra W., van der Zeijst B. A. M. 1990; Size and physical map of the Campylobacter jejuni chromosome.. Nucleic Acids Res 18:6211–6214
    [Google Scholar]
  22. Owen R. J., Hernandez J. 1990; Genotypic variation in Campylobacter upsaliensis from blood and faeces of patients in different countries.. FEMS Microbiol Eett 72:5–10
    [Google Scholar]
  23. Patton C.M., Shaffer N., Edmonds P., Barrett T.J., Lambert M.A., Baker C., Perlman D.M., Brenner D.J. 1989; Human disease associated with ' Campylobacter upsaliensis' (catalase-negative or weakly positive Campylobacter species) in the United States.. Clin Microbiol 27:66–73
    [Google Scholar]
  24. Pyle L.E., Corcoran L.N., Cocks B.G., Bergemann A.D., Whitley J.C., Finch L.R. 1988; Pulsed-field electrophoresis indicates larger-than-expected sizes for mycoplasma genomes.. Nucleic Acids Res 16:6015–6025
    [Google Scholar]
  25. Romling U., Grothues D., Bautsch W., Tummler B. 1989; A physical genome map of Pseudomonas aeruginosa PAO.. EMBO J 8:4081–4089
    [Google Scholar]
  26. Salama S.M., Taylor D.E. 1993; Construction of a genomic map of the chromosome of Campylobacter fetus subsp.fetus ATCC 27374.. Acta Gastro-enterol Belg 56:18
    [Google Scholar]
  27. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Eaboratory Manual,, 2. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  28. Sandstedt K., Ursing J. 1991; Description of the Campylobacter upsaliensis sp. nov. previously known as the CNW group.. Syst Appl Microbiol 14:39–45
    [Google Scholar]
  29. Sandstedt K., Ursing J., Walder M. 1983; Thermotolerant Campylobacter with no or weak catalase activity isolated from dogs.. Curr Microbiol 8:209–213
    [Google Scholar]
  30. Smith C.L., Econome J.G., Schutt A., Kico S., Cantor C.R. 1987; A physical map of the Escherichia coli K12 genome.. Science 236:1448–1453
    [Google Scholar]
  31. Smith C.L., Klco S.R., Cantor C.R. 1988; Pulsed-field gel electrophoresis and the technology of large DNA molecules.. In Genome Analysis: a Practical Approach, pp. 41–69 Davis. K. E. Edited by Oxford:: IRL Press.;
    [Google Scholar]
  32. Steele T.W., Sangster N., Lanser J.A. 1985; DNA relatedness and biochemical features of Campylobacter spp. isolated in Central and South Australia.. J Clin Microbiol 22:71–74
    [Google Scholar]
  33. Taylor D.E., Hiratsuka K., Mueller L. 1989; Isolation and characterization of catalase-negative and catalase-weak strains of Campylobacter species, including 'Campylobacter upsaliensis', from humans with gastroenteritis.. J Clin Microbiol 27:2042–2045
    [Google Scholar]
  34. Thomas C.A., Berns K.I., Kelly T.J. 1966; Isolation of high molecular weight DNA from bacteria and cell nuclei.. In Procedures in Nucleic Acid Research, pp. 535–540 Cantoni G. L., Druve. D. R. Edited by New York:: Harper & Row.;
    [Google Scholar]
  35. Walmsiey S.L., Karmali M.A. 1989; Direct isolation of atypical thermophilic Campylobacter species from human feces on selective agar medium.. J Clin Microbiol 27:668–670
    [Google Scholar]
  36. Yan W., Taylor D.E. 1991; Sizing and mapping of the genome of Campylobacter coli strain UA417R using pulsed-field gel electrophoresis.. Gene 101:117–120
    [Google Scholar]
/content/journal/micro/10.1099/13500872-141-10-2417
Loading
/content/journal/micro/10.1099/13500872-141-10-2417
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error