1887

Abstract

transports mannose by a phosphoenolpyruvate: sugar phosphotransferase system (PTS) which consists of a membrane Enzyme II and two forms of Enzyme III (III) with molecular masses of 38.9 kDa (III ) and 35.2 kDa (III ) respectively. Using a pseudorevertant (strain 57P) isolated from a III -deficient spontaneous mutant unable to grow on mannose, we demonstrated that could also transport mannose by an inducible fructose PTS. This PTS phosphorylated fructose at the C-1 position with a high affinity (10 μM) and mannose at the C-6 position with a low affinity (200 μM). Derepression of this system in some III -deficient mutants would explain their ability to grow on mannose.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-140-9-2433
1994-09-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/140/9/mic-140-9-2433.html?itemId=/content/journal/micro/10.1099/13500872-140-9-2433&mimeType=html&fmt=ahah

References

  1. Bourassa S., Vadeboncoeur C. 1992; Expression of an inducible enzyme II fructose and activation of a cryptic enzyme II glucose in glucose-grown cells of spontaneous mutants of Streptococcus salivarius lacking the low-molecular-mass form of IIIMan, a component of the phosphoenolpyruvate: mannose phosphotransferase system. J Gen Microbiol 138:769–777
    [Google Scholar]
  2. Bourassa S., Gauthier L., Giguere R., Vadeboncoeur C. 1990; A IVMan protein is involved in the transport of glucose, mannose and fructose by oral streptococci.. Oral Microbiol Immunol 5:288–297
    [Google Scholar]
  3. Brochu D., Trahan L., Jacques M., Lavoie M. C., Frenette M., Vadeboncoeur C. 1993; Alterations in the cellular envelope of spontaneous IIIManL-defective mutants of Streptococcus salivarius.. J Gen Rficrobioll 39:1291–1300
    [Google Scholar]
  4. Dixon M. 1953; The determination of enzyme inhibition constants. Biocbem. J 55:170–177
    [Google Scholar]
  5. Gauthier L., Bourassa S., Brochu D., Vadeboncoeur C. 1990; Control of sugar utilization in oral streptococci. Properties of phenotypically distinct 2-deoxyglucose-resistant mutants of Streptococcus salivarius.. Oral Microbiol Immunol 5:352–359
    [Google Scholar]
  6. Gracy R.W., Noltman E. A. 1968; Studies on phosphomannose isomerase. Isolation, homogeneity measurements, and determination of some physical properties. J Biol Chem 243:3161–3168
    [Google Scholar]
  7. Hamilton I. R., Gauthier L., Desjardins B., Vadeboncoeur C. 1989; Concentration-dependent repression of the soluble and membrane components of the Streptococcus mutans phosphoenol-pyruvate: sugar phosphotransferase system by glucose. J Bacteriol 171:2942–2948
    [Google Scholar]
  8. Kornberg H.S., Lambourne L. T. M. 1992; Role of the phosphoenolpyruvate-dependent fructose phosphotransferase system in the utilisation of mannose by Escherichia coli.. Proc R Soc Lond
    [Google Scholar]
  9. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  10. Lapointe R., Frenette M., Vadeboncoeur C. 1993; Altered expression of several genes in IIIman-defective mutants of Streptococcus salivarius demonstrated by two-dimensional gel electrophoresis of cytoplasmic proteins.. Res Microbiol 144:305–316
    [Google Scholar]
  11. Mattoo R.S., Waygood E. B. 1983; An enzymatic method for [32PEP]phosphoenolpyruvate synthesis.. Anal Biochem 128:245–249
    [Google Scholar]
  12. Meadow N. D., Fox D. K., Roseman S. 1990; The bacterial phosphoenolpyruvate: glycose phosphotransferase system. Annu Rev Biochem 59:497–542
    [Google Scholar]
  13. Postma P. W., Lengeler J. W., Jacobson G. R. 1993; Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria. Microbiol Rev 57:543–594
    [Google Scholar]
  14. Rodrigue L., Lacoste L., Trahan L., Vadeboncoeur C. 1988; Effect of nutritional constraints on the biosynthesis of the components of the phosphoenolpyruvate: sugar phospho-transferase system in a fresh isolate of Streptococcus mutans.. Infect Irnmun 56:518–522
    [Google Scholar]
  15. Thompson J. 1987; Sugar transport in the lactic acid bacteria. In Sugar Transport and Metabolism in Gram-positive Bacteria pp. 13–38 Edited by Reizer J., Peterkofsky A. Chichester: Ellis Horwood;
    [Google Scholar]
  16. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
    [Google Scholar]
  17. Vadeboncoeur C. 1984; Structure and properties of the phosphoenolpyruvate: glucose phosphotransferase system of oral streptococci. Can J Microbiol 30:495–502
    [Google Scholar]
  18. Vadeboncoeur C., Gauthier L. 1987; The phosphoenol-pyruvate : sugar phosphotransferase system of Streptococcus salivarius. Identification of a IHMan protein.. Can J Microbiol 33:118–122
    [Google Scholar]
  19. Vadeboncoeur C., Trahan L. 1982; Glucose transport in Streptococcus salivarius. Evidence for the presence of a distinct hosphoenolpyruvate: glucose phosphotransferase system which catalyses the phosphorylation of alpha-methyl glucoside.. Can J Microbiol 28:190–199
    [Google Scholar]
  20. Waygood E. B., Mattoo R. L., Erickson E., Vadeboncoeur C. 1986; Phosphoproteins and the phosphoenolpyruvate: sugar phosphotransferase system of Streptococcus salivarius. Detection of two different ATP-dependent phosphorylations of the phospho-carrier protein fd Pr. . Can J Microbiol 32:310–318
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-140-9-2433
Loading
/content/journal/micro/10.1099/13500872-140-9-2433
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error