1887

Abstract

transports mannose by a phosphoenolpyruvate: sugar phosphotransferase system (PTS) which consists of a membrane Enzyme II and two forms of Enzyme III (III) with molecular masses of 38.9 kDa (III ) and 35.2 kDa (III ) respectively. Using a pseudorevertant (strain 57P) isolated from a III -deficient spontaneous mutant unable to grow on mannose, we demonstrated that could also transport mannose by an inducible fructose PTS. This PTS phosphorylated fructose at the C-1 position with a high affinity (10 μM) and mannose at the C-6 position with a low affinity (200 μM). Derepression of this system in some III -deficient mutants would explain their ability to grow on mannose.

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1994-09-01
2024-04-25
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