1887

Abstract

We have characterized a new family of proteins (the ROK family) which includes six transcriptional repressors for sugar catabolic operons, three sugar kinases, and three unidentified open reading frames. Analyses of the aligned sequences and phylogenetic tree construction allow predictions regarding the functional nature of conserved domains and residues within these proteins as well as the pathway of evolutionary divergence that gave rise to the family.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-140-9-2349
1994-09-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/140/9/mic-140-9-2349.html?itemId=/content/journal/micro/10.1099/13500872-140-9-2349&mimeType=html&fmt=ahah

References

  1. Angell S., Schwarz E., Bibb M. J. 1992; The glucose kinase gene of Streptomyces coeiicolor A3(2): its nucleotide sequence, transcriptional analysis and role in glucose repression.. Mol Alicrobiol 6:2833–2844
    [Google Scholar]
  2. Blattner F. R., Burland V. D., Plunkett G., Sofia H. J., Daniels D. L. 1993; Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89-2 to 92-8 minutes.. Nucleic Acids 21:5408–5417
    [Google Scholar]
  3. Bork P., Sander C., Valencia A. 1992; An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin, and hsp70 heat shock proteins. Proc Natl Acad Sci USA 89:7290–7294
    [Google Scholar]
  4. Bork P., Sander C., Valencia A. 1993; Convergent evolution of similar enzymatic function on different protein folds: the hexo-kinase, ribokinase, and galactokinase families of sugar kinases. Prot Sci 2:31–40
    [Google Scholar]
  5. Dodd I.B., Egan J. B. 1990; Improved detection of helix-turn-helix DNA-binding motifs in protein sequences. Nucleic Acids Res 18:5019–5026
    [Google Scholar]
  6. Doolittle R.F., Bork P. 1993; Evolutionarily mobile modules in proteins. Sci Am50–56
    [Google Scholar]
  7. Feng D.-F., Doolittle R. F. 1990; Progressive alignment and phylogenetic tree construction of protein sequences. Methods Enzymol 183:375–387
    [Google Scholar]
  8. Kreuzer P., Gaertner D., Allmansberger R., Hillen W. 1989; identification and sequence analysis of the 'Bacillus subtilis W23 xyl R gene and xyl operator. J Bacteriol 171:3840–3845
    [Google Scholar]
  9. Lokman B.C., van Santen P., Verdoes J., Kruese J., Leer F.I.J., Posno M., Pouwels P. H. 1991; Organization and characterization of three genes involved in D-xylose catabolism in Lactobacillus pentosus.. Mol and Gen Genet 230:161–169
    [Google Scholar]
  10. Pearson W.R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85:2444–2448
    [Google Scholar]
  11. Plumbridge J. 1989; Sequence of the nag BACD operon in E. coli K.12 and pattern of transcription within the nag regulon. Mol Microbiol 3:505–515
    [Google Scholar]
  12. Reeder T., Schleif R. 1991; Mapping, sequence, and apparent lack of function of araj a gene of the Escherichia coli arabinose regulon.. J Bacteriol 173:7765–7771
    [Google Scholar]
  13. Reizer J., Reizer A., Saier M. H. 1993; Exopolyphosphate phosphatase and guanosine pentaphosphate phosphatase belong to the sugar kinase/actin/hsp70 superfamily. TIBS 18:247–248
    [Google Scholar]
  14. Rygus T., Scheler A., Allmansberger R., Hillen W. 1991; Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization. Arch Microbiol 155:535–542
    [Google Scholar]
  15. Saier M. H. 1994; Computer-aided analyses of transport protein sequences: gleaning evidence concerning function, structure, biogenesis, and evolution. Microbiol Rev 58:71–93
    [Google Scholar]
  16. Saier M. H., Jacobson G. R. 1984; The Molecular Basis of Sex and Differentiation: a Comparative Study of Evolution. Mechanism and Control in Microorganisms. New York: Springer-Verlag;
    [Google Scholar]
  17. Sato Y., Yamamoto Y., Kizaki H., Kuramitsu H. K. 1993; Isolation, characterization and sequence analysis of the scr K gene encoding fructokinase of Streptococcus mutans. J Gen Microbiol 139:921–927
    [Google Scholar]
  18. Scheler A., Rygus T., Allmansberger R., Hillen W. 1991; Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus licheniformis encoded regulon for xylose utilization.. Arch Microbiol 155:526–534
    [Google Scholar]
  19. Sizemore C., Buchner E., Rygus T., Witke C., Gotz F., Hillen W. 1991; Organization, promoter analysis and transcriptional regulation of the Staphylococcus xylosus xylose utilization operon. Mol and Gen Genet 227:377–384
    [Google Scholar]
  20. Tam R., Saier M. H. 1993a; Structural, functional, and evolutionary relationships among extracellular solute-binding receptors of bacteria. Microbiol Rev 57:320–346
    [Google Scholar]
  21. Tam R., Saier M. H. 1993b; A bacterial periplasmic receptor homologue with catalytic activity: cyclohexadienyl dehydratase of Pseudomonas aeruginosa is homologous to receptors specific for polar amino acids.. Res Microbiol 144:165–169
    [Google Scholar]
  22. Vartak N. B., Reizer J., Reizer A., Gripp J. T., Groisman E. A., Wu L.-F., Tomich J. M., Saier M. H. 1991; Sequence and evolution of the Fru R protein of Salmonella typhimurium: a pleiotropic transcriptional regulatory protein possessing both activator and repressor functions which is homologous to the periplasmic ribose-binding protein.. Res Microbiol 142:951–963
    [Google Scholar]
  23. Weickert M.J., Adhya S. 1992; A family of bacterial regulators homologous to Gal and Lac repressors. J Biol Chem 267:15869–15874
    [Google Scholar]
  24. Wilson S., Drew R. 1991; Cloning and DNA sequence of amiC, a new gene regulating expression of the Pseudomonas aeruginosa aliphatic amidase, and purification of the amiC product. J Bacteriol 173:4914–4921
    [Google Scholar]
  25. Zembrzuski B., Chilco P., Liu X.-L., Liu J., Conway T., Scopes R. K. 1992; The fructokinase gene from Zymomonas mobilis: cloning, sequencing, expression and structural comparison of the enzyme with other hexose kinases. J Bacteriol 174:3455–3460
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-140-9-2349
Loading
/content/journal/micro/10.1099/13500872-140-9-2349
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error