The molecular characteristics of the ethylene-forming enzymes of strains of were tested. The ethylene-producing activities of the nine strains as measured and were similar, except for that of pv. M5. A polyclonal antibody and a DNA probe for the ethylene-forming enzyme from pv. PK2 were prepared to investigate homologies among the proteins and genes for the ethylene-forming enzymes. With the exception of pv. M5, eight strains tested expressed the same antigen as the ethylene-forming enzyme from pv. PK2 and were homologous to DNA sequences on indigenous plasmids. Molecular masses of antigenic proteins from all ethylene-producing strains were 40 kDa. The N-terminal amino acid sequence of the purified ethylene-forming enzyme from pv. KN130 was identical to that of the enzyme from pv. PK2. These results show that the ethylene-forming enzymes encoded by the indigenous plasmid(s) in the pathogenic bacteria examined were similar.


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