1887

Abstract

The molecular characteristics of the ethylene-forming enzymes of strains of were tested. The ethylene-producing activities of the nine strains as measured and were similar, except for that of pv. M5. A polyclonal antibody and a DNA probe for the ethylene-forming enzyme from pv. PK2 were prepared to investigate homologies among the proteins and genes for the ethylene-forming enzymes. With the exception of pv. M5, eight strains tested expressed the same antigen as the ethylene-forming enzyme from pv. PK2 and were homologous to DNA sequences on indigenous plasmids. Molecular masses of antigenic proteins from all ethylene-producing strains were 40 kDa. The N-terminal amino acid sequence of the purified ethylene-forming enzyme from pv. KN130 was identical to that of the enzyme from pv. PK2. These results show that the ethylene-forming enzymes encoded by the indigenous plasmid(s) in the pathogenic bacteria examined were similar.

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1994-09-01
2022-01-16
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References

  1. Adams D.O., Yang S. F. 1979; Ethylene biosynthesis: identification of 1-aminocyclopropane-l-carboxylic acid as an intermediate in the conversion of methionine to ethylene. Proc Natl Acad Sci USA 76:170–174
    [Google Scholar]
  2. Birnboim H.C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
    [Google Scholar]
  3. Coplin D. L., Rowan R. G., Chisholm D. A., Whitmoyer R. E. 1981; Characterization of plasmids in Erwinia stewartii. Appl Environ Microbiol 42:599–604
    [Google Scholar]
  4. Curiale M.S., Mills D. 1983; Molecular relatedness among cryptic plasmids in Pseudomonas syringae pv. glycinea.. Phytopathology 73:1440–1444
    [Google Scholar]
  5. Freebairn H.T., Buddenhagen I. W. 1964; Ethylene production by Pseudomonas solanacearum.. Nature 202:313–314
    [Google Scholar]
  6. Fukuda H., Ogawa T. 1992; Microbial production. In The Plant Hormone Ethylene pp. 279–292 Edited by Matto A. K., Suttle J. C. . London: CRC Press;
    [Google Scholar]
  7. Fukuda H., Fujii T., Ogawa T. 1986; Preparation of a cell-free ethylene-forming system from Penicillium digitatum.. Agric Biol Chem 50:977–981
    [Google Scholar]
  8. Fukuda H., Kitajima H., Fujii T., Tazaki M., Ogawa T. 1989a; Purification and some properties of a novel ethylene-forming enzyme produced by Penicillium digitatum. FEMS Microbiol Lett 59:1–6
    [Google Scholar]
  9. Fukuda H., Takahashi M., Fuji T., Tazaki M., Ogawa T. 1989b; An NADH: Fe(III)EDTA oxidoreductase from Crypto-coccus albidus-. an enzyme involved in ethylene production in vivo?. FEMS Microbiol Eett 60:107–112
    [Google Scholar]
  10. Fukuda H., Ogawa T., Ishihara K., Fujii T., Nagahama K., Omata T., Inoue Y., Tanase S., Morino Y. 1992a; Molecular cloning in Escherichia coli expression and nucleotide sequence of the gene for the ethylene-forming enzyme of Pseudomonas syringae pv. phaseolicoln PK2.. Biochem Biophys Res Commun 188:826–832
    [Google Scholar]
  11. Fukuda H., Ogawa T., Tazaki M., Nagahama K., Fujii T., Tanase S., Morino Y. 1992b; Two reactions are simultaneously catalyzed by a single enzyme: the arginine-dependent simultaneous formation of two products, ethylene and succinate, from 2-oxoglutarate by an enzyme from Pseudomonas syringae. Biochem Biophys Res Commun 188:483–489
    [Google Scholar]
  12. Fukuda H., Ogawa T., Tanase S. 1993; Ethylene production by microorganisms. In Advances in Microbial Physiology pp. 275–306 Edited bv A. H. Rose. London: Academic Press;
    [Google Scholar]
  13. Goto M., Ishida Y., Takikawa Y., Hyodo H. 1985; Ethylene production by the kudze strains of Pseudomonas syringae pv. phaseolicola causing halo blight in Pueraria lobata (Willd) Ohwi.. Plant Cell Physiol 26:141–150
    [Google Scholar]
  14. Laemmli U.K. 1970; Cleavage of structural proteins during the assemblv of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  15. Meinkoth J., Wahl G. 1984; Elybridization of nucleic acids immobilized on solid supports. Anal Biochem 138:267–284
    [Google Scholar]
  16. Nagahama K., Ogawa T., Fujii T., Tazaki M., Goto M., Fukuda H. 1991a; l-Arginine is essential for the formation in vitro of ethylene by an extract of Pseudomonas syringae. J Gen Microbiol 137:1641–1646
    [Google Scholar]
  17. Nagahama K., Ogawa T., Fujii T., Tazaki M., Tanase S., Morino Y., Fukuda H. 1991b; Purification and properties of an ethylene-forming enzyme from Pseudomonas syringae. J Gen Microbiol 137:2281–2286
    [Google Scholar]
  18. Nishiyama K., Azegami K., Osada S., Nakasone W., Ezuka A., Watanabe Y. 1986; Bacterial diseases of soybean and their pathogens in Japan. Bull Natl Inst Agro-Environ Sci 1:83–94
    [Google Scholar]
  19. Ogawa T., Takahashi M., Fujii T., Tazaki M., Fukuda H. 1990; The role of NADH:Fe(III)EDTA oxidoreductase in ethylene formation from 2-keto-4-methylthiobutyrate. J Ferment Bioeng 93:177–181
    [Google Scholar]
  20. Sato M. 1983; Phag e-induction from lysogenic strains of Pseudomonas syringae pv. mori by an extract from mulberry leaves.. Ann Phytopathol Soc Japan 49:259–261
    [Google Scholar]
  21. Sato M., Urushizaki S., Nishiyama K., Sakai F., Ota Y. 1987; Efficient production of ethylene by Pseudomonas syringae pv. glycinea which causes halo blight in soybeans.. Agric Biol Chem 51:1117–1178
    [Google Scholar]
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