@article{mbs:/content/journal/micro/10.1099/13500872-140-9-2289, author = "Yoshida, K. and Sano, H. and Miwa, Y. and Ogasawara, N. and Fujita, Y.", title = "Cloning and nucleotide sequencing of a 15 kb region of the Bacillus subtilis genome containing the iol operon", journal= "Microbiology", year = "1994", volume = "140", number = "9", pages = "2289-2298", doi = "https://doi.org/10.1099/13500872-140-9-2289", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-140-9-2289", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "genomic sequencing", keywords = "ABC family", keywords = "inositol operon", keywords = "Bacillus subtilis", keywords = "OmpR subfamily", abstract = "Within the framework of an international project on the sequencing of the whole Bacillus subtilis genome, a 15 kb chromosome segment, which contains the iol operon involved in inositol utilization, has been cloned and sequenced. This region (14974 bp) contains 12 complete open reading frames (ORFs; genes) and two partial ones; the seventh gene (E83G) is the idh gene encoding inositol dehydrogenase. All the genes identified are transcribed in the same direction as that of the movement of the replication fork. A homology search for their products deduced from the 12 complete ORFs revealed that eight of them exhibit significant homology to known proteins such as fructokinase, acetolactate synthase, fructose-1,6-bisphosphate aldolase (B. subtilis), and PhoB and FtsE proteins (Escherichia coli). It also implied that two genes (B65B and B65E) might encode a set of two-component regulatory proteins and that the B65F gene might encode a protein belonging to the ATP-binding cassette (ABC) family. Based on the features of the nucleotide sequence determined and the results of the homology search, the primary structure of the iol operon is predicted.", }