1887

Abstract

Within the framework of an international project on the sequencing of the whole genome, a 15 kb chromosome segment, which contains the operon involved in inositol utilization, has been cloned and sequenced. This region (14974 bp) contains 12 complete open reading frames (ORFs; genes) and two partial ones; the seventh gene () is the gene encoding inositol dehydrogenase. All the genes identified are transcribed in the same direction as that of the movement of the replication fork. A homology search for their products deduced from the 12 complete ORFs revealed that eight of them exhibit significant homology to known proteins such as fructokinase, acetolactate synthase, fructose-1,6-bisphosphate aldolase (), and PhoB and FtsE proteins (). It also implied that two genes ( and ) might encode a set of two-component regulatory proteins and that the gene might encode a protein belonging to the ATP-binding cassette (ABC) family. Based on the features of the nucleotide sequence determined and the results of the homology search, the primary structure of the operon is predicted.

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/content/journal/micro/10.1099/13500872-140-9-2289
1994-09-01
2019-11-17
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-140-9-2289
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