RT Journal Article SR Electronic(1) A1 Park, Jong Hoon A1 Song, Jae-Chan A1 Kim, Myoung Hee A1 Lee, Dae-Sil A1 Kim, Cheorl-HoYR 1994 T1 Determination of genome size and a preliminary physical map of an extreme alkaliphile, Micrococcus sp. Y-1, by pulsed-field gel electrophoresis JF Microbiology, VO 140 IS 9 SP 2247 OP 2250 DO https://doi.org/10.1099/13500872-140-9-2247 PB Microbiology Society, SN 1465-2080, AB Large restriction fragments of genomic DNA from Micrococcus sp. Y-1 were separated by pulsed-field gel electrophoresis (PFGE). Since Micrococcus sp. Y-1 has a G + C content of approximately 70%, restriction fragments were obtained by digesting chromosomal DNA with endonucleases which recognize A+T-rich sequences. Five enzymes, Sspl, Spel, Xbal, Hpal and EcoRI, were used for generation of distinctly separated fragments in the size range 100-500 kb. No site for DraI was detected. In contrast, sites for 8-base-recognizing enzymes, but not for NotI and Sfil, were frequent. The genome size of Micrococcus sp. Y-1 was determined from restriction fragments separated by PFGE, and was estimated to be approximately 4061 kb. Partial digestion experiments revealed the order of the six Sspl fragments on the chromosome., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-140-9-2247