Large restriction fragments of genomic DNA from sp. Y-1 were separated by pulsed-field gel electrophoresis (PFGE). Since sp. Y-1 has a G + C content of approximately 70%, restriction fragments were obtained by digesting chromosomal DNA with endonucleases which recognize A+T-rich sequences. Five enzymes, l, l, l, l and RI, were used for generation of distinctly separated fragments in the size range 100-500 kb. No site for I was detected. In contrast, sites for 8-base-recognizing enzymes, but not for I and l, were frequent. The genome size of sp. Y-1 was determined from restriction fragments separated by PFGE, and was estimated to be approximately 4061 kb. Partial digestion experiments revealed the order of the six l fragments on the chromosome.


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