1887

Abstract

Sexual agglutination, caused by agglutination substance (AS) on a and α cell walls, is the first indispensable step of the mating reaction in ascosporogenous yeasts including . The AS biosynthetic process in was investigated by pulse label-chase experiments with analysis by polyacrylamide gel electrophoresis (PAGE) for 16 h in the presence of urea. Because of its low mobility, AS can be separated from other proteins by prolonged PAGE. Nascent AS was integrated into cell walls after it linked covalently to a ‘carrier’ glycoprotein. The results suggest that the ‘carrier’ is synthesized stepwise through three distinct precursors (III → II → I). The ‘carrier’ glycoprotein (I) and its precursors (II, III) were synthesized in both a, α haploid and a/α diploid cells. The -glycosylation linkage inhibitor, tunicamycin, and protein synthesis inhibitor, puromycin, inhibited the III to I maturation. The results indicated that both the ‘carrier’ and the nascent active site of AS linked to the ‘carrier’ are integrated into the wall in a haploid cell while the ‘carrier’ alone is integrated in a diploid cell.

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1994-09-01
2024-04-23
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References

  1. Cappeilaro C., Hauser K., Mrsa V., Watzele M., Watzele G., Gruber G., Tanner W. 1991; Saccharomjces cerevisiae a-and a-agglutinin: characterization of their molecular interaction.. EMBO J 10:4081–4088
    [Google Scholar]
  2. Lipke P. N., Wojciechowicz D., Kurjan J. 1989; AGa1 is the structural gene for the Saccharomjces cerevisiae a-agglutinin, a cell surface glycoprotein involved in cell-cell interactions during mating. Mol Cell Biol 9:3155–3165
    [Google Scholar]
  3. MacKay V. L., Manney T. R. 1974; Mutations affecting sexual conjugation and related processes in Saccharomjces cerevisiae. II. Genetic analysis of nonmating mutants.. Genetics 76:273–288
    [Google Scholar]
  4. McCaffrey G., Clay F. J., Kelsay K., Sprague G. F. 1987; Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae.. Mol Cell Biol 7:2680–2690
    [Google Scholar]
  5. Melcher U. 1971; Metabolism of puromycin by yeast cells. Biochim Biophys Acta 246:216–224
    [Google Scholar]
  6. Novick P., Ferro S., Schekman R. 1981; Order of events in the yeast secretory pathway. Cell 25:461–469
    [Google Scholar]
  7. Stevens T., Esmon B., Schekman R. 1982; Early stages in the yeast secretory pathway are required for transport of carboxypeptidase Y to the vacuole. Cell 30:439–448
    [Google Scholar]
  8. Strathern J. N., Hicks J. B., Herskowitz I. 1981; Control of cell type in yeast by the mating type locus: the a1-a2 hypothesis. J Mol Biol 147:357–372
    [Google Scholar]
  9. Suzuki K., Yanagishima N. 1986; Genetic characterization of an α-specific gene responsible for sexual agglutinability in Saccharomjces cerevisiae. mapping and gene dose effect.. Curr Genet 10:353–357
    [Google Scholar]
  10. Tohoyama H., Hagiya M., Yoshida K., Yanagishima N. 1979; Regulation of the production of the agglutination substances responsible for sexual agglutination in Saccharomjces cerevisiae.. Mol and Gen Genet 174:269–280
    [Google Scholar]
  11. Tohoyama H., Yanagishima N. 1982; Control of the production of the sexual agglutination substances by the mating type locus in Saccharomjces cerevisiae.. Mol and Gen Genet 186:322–327
    [Google Scholar]
  12. Tohoyama H., Yanagishima N. 1987; Site of pheromone action and secretion pathway of a sexual agglutination substance during its induction by pheromone a in a cells of Saccharomyces cerevisiae. Curr Genet 12:271–275
    [Google Scholar]
  13. Truehart J., Boeke J. D., Fink G. R. 1987; Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein. Mol Cell Bioll2316–2328
    [Google Scholar]
  14. Watzele M., Klis F., Tanner W. 1988; Purification and characterization of the inducible a agglutinin of Saccharomyces cerevisiae.. EMBO J 7:1483–1488
    [Google Scholar]
  15. Wojciechowicz D., Lipke P. N. 1989; α-Agglutinin expression in Saccharomyces cerevisiae.. Biochem Biophys Res Commun 161:46–51
    [Google Scholar]
  16. Yamaguchi M., Yoshida K., Yanagishima N. 1982; Isolation and partial characterization of cytoplasmic α agglutination substance in the yeast Saccharomyces cerevisiae. FEBS Eett 139:125–129
    [Google Scholar]
  17. Yamaguchi M., Yoshida K., Yanagishima N. 1984; Isolation, and biochemical and biological characterization of an α-mating-type-specific glycoprotein responsible for sexual agglutination from the ectoplasm of alpha-cells, in the yeast Saccharomyces cerevisiae.. Arch Microbiol 140:113–119
    [Google Scholar]
  18. Yanagishima N. 1984; Mating systems and sexual interactions in yeast. In Cellular Interactions (Encyclopedia of Plant Physiology, New Series) pp. 402–423 Edited by Linskens H.F., Heslop-Harrison J. Berlin and Heidelberg: Springer;
    [Google Scholar]
  19. Yanagishima N., Yoshida K. 1981; Sexual interaction in Saccharomyces cerevisiae with special reference to the regulation of sexual agglutinability. In Sexual Interactions in Eucaryotic Microbes pp. 261–295 Edited by O’Day D. H., Horgen P. A. . New York: Academic Press;
    [Google Scholar]
  20. Yoshida K., Hagiya M., Yanagishima N. 1976; Isolation and purification of the sexual agglutination substance of mating type a cells in Saccharomyces cerevisiae. Biochem Biophys Res Commun 71:1085–1094
    [Google Scholar]
  21. Yoshida K., Hisatomi T., Yanagishima N. 1989; Sexual behavior and its pheromonal regulation in ascosporogenous yeasts. J Basic Microbiol 29:99–128
    [Google Scholar]
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