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Volume 140, Issue 8

Evolutionary affiliation of the marine nitrogen-fixing cyanobacterium sp. strain NIBB 1067, derived by 16S ribosomal RNA sequence analysis Free

Abstract

The 16S rRNA sequence of sp. strain NIBB 1067 was determined and used for the construction of a distance tree and bootstrap analysis. The tree shows that, among the available cyanobacterial 16S rRNA sequences, NIBB 1067 has PCC 7515 as its closest relative, presenting 94.9% of sequence similarity with the latter strain. This is in contrast to a difference of 9 mol% G + C in mean genomic DNA base composition between the two organisms. Nevertheless, the genotypic heterogeneity presented by a number of strains assigned to the genus hinders a taxonomic decision on the separate existence of the genera and The sequence of the internal transcribed spacer (ITS) between the 16S and 23S rRNA genes was also determined, as a possible marker to study inter- and intraspecific variability. The ITS contains the genes coding for tRNA and tRNA and its total length is 547 nucleotides. In six out of eight sequenced clones, there is a duplication of 29 nucleotides, surrounding the 5′ end of the tRNA.

  • Received:
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Keyword(s): 16S rRNA sequence analysis , cyanobacteria , internal transcribed spacer , taxonomy and Trichodesmium
© Society for General Microbiology, 1994
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1994-08-01
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Evolutionary affiliation of the marine nitrogen-fixing cyanobacterium Trichodesmium sp. strain NIBB 1067, derived by 16S ribosomal RNA sequence analysis
Microbiology 140, 2159 (1994); https://doi.org/10.1099/13500872-140-8-2159
/content/journal/micro/10.1099/13500872-140-8-2159
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