1887

Abstract

We report the first quantitative analysis of the relationship between environmental changes and sporulation of a streptomycete, SMF301, in submerged culture. A chemically defined medium was constructed for sporulation, over 10 spores ml being formed in the submerged batch culture. Kinetic parameters calculated from batch and chemostat cultures showed that specific submerged spore formation rate (q) was inversely related to the specific mycelial growth rate (μ). The optimum growth rate for submerged spore formation was 0.05 h, when the maximum value of q was 1.0 × 10 spores g h. The turnover rate of biomass at maximum growth yield was 0.029 h when 5.6 × 10 spores were formed from 1 g mycelium. The present quantitative analysis of submerged spore formation using a controlled system opens the way for biochemical and molecular biological studies related to the morphological differentiation of spp.

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1994-08-01
2021-07-26
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References

  1. Babcock M.J., Kendrick K.E. Cloning of DNA involved in sporulation of Streptomyces griseus. J bacteriol 1988; 170:2802–2808
    [Google Scholar]
  2. Champness W.C. New loci required for Streptomyces coelicolor morphological and physiological differentiation. J bacteriol 1988; 170:1168–1174
    [Google Scholar]
  3. Champness W.C., Adamidis T., Riggle P.E. Developmental genes and antibiotic biosynthesis. In Genetics and Molecular biology of Industrial Microorganisms 1989 Edited by Flershberger C.L., Queener S.W., Hegeman G. Washington, DC: American Society for Microbiology; pp 108–112
    [Google Scholar]
  4. Chater K.F. Morphological and physiological differentiation in Streptomyces. In Microbial Development 1984 Edited by Losick R., Shapiro L. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; pp 89–115
    [Google Scholar]
  5. Chater K.F. Sporulation in Streptomyces. In Regulation of Prokaryotic Development 1989 Edited by Smith I., Slepecky R.A., Setlow P. Washington, DC: American Society for Microbiology; pp 277–299
    [Google Scholar]
  6. Chater K.F. Sap, hydrophobins and aerial growth. Curr biol 1991; 1:318–320
    [Google Scholar]
  7. Chater K.F., Lawlor E.J., Mendez C., Bruton C.J., Davis N.K., Plaskitt K., Guthrie E.P., Daly B.L., Baylis H.A., Trong K.V. Gene expression during Streptomyces development. In Biology of Actinomycetes '88. Proceedings of the 7th International Symposium for biology of Actinomycetes 1988 Edited by Okami Y. Tokyo: Japan Scientific Societies Press; pp 64–70
    [Google Scholar]
  8. Chater K.F., Bruton C.J., Plaskitt K.A., Buttner M.J., Mendez C., Helmann J. The developmental fate of Streptomyces coelicolor hyphae depends crucially on a gene product homologous with motility sigma factor of bacillus subtilis. Cell 1989; 59:133–143
    [Google Scholar]
  9. Daza A., Martin J.F., Dominguez A., Gil J.A. Sporulation of several species of Streptomyces in submerged culture after nutritional downshift. J Gen Microbiol 1989; 135:2483–2491
    [Google Scholar]
  10. Demain A.L. Catabolic regulation in industrial microbiology. In Overproduction of Microbial Products 1982 Edited by Krumphanzl V., Sikya B., Vanek Z. New York: Academic Press; pp 3–20
    [Google Scholar]
  11. Glazebrook M.A., Doull J.L., Stuttard C., Vining L.C. Sporulation of Streptomyces venezuelae in submerged cultures. J Gen Microbiol 1990; 136:581–588
    [Google Scholar]
  12. Huber F.M., Piper R.L., Mertz F.P. Sporulation of Streptomyces roseosporus in submerged culture. J Ind Microbiol 1987; 2:235–241
    [Google Scholar]
  13. Jeong B.C., Shin H.S., Lee K.J. Relationship between sporulation and synthesis of alkaline protease in a Streptomyces sp. Korean J Microbiol 1988; 26:355–361
    [Google Scholar]
  14. Kendrick K.E., Ensign J.C. Sporulation of Streptomyces griseus in submerged culture. J bacteriol 1983; 155:357–366
    [Google Scholar]
  15. Koepsel R., Ensign J.C. Microcycle sporulation of Streptomyces viridochromogenes. Arch Microbiol 1984; 140:9–14
    [Google Scholar]
  16. Lee K.J., Rho Y.T. Characteristics of spores formed by surface and submerged cultures of Streptomyces albidoflavus SMF301. J Gen Microbiol 1993; 139:3131–3137
    [Google Scholar]
  17. Miller G.L. Use of dinitrosalycilic acid reagent for determination of reducing sugar. Anal Chem 1959; 31:426–428
    [Google Scholar]
  18. Ochi K. Changes in nucleotide pools during sporulation of Streptomyces griseus in submerged culture. J Gen Microbiol 1987; 133:2787–2795
    [Google Scholar]
  19. Pirt S.J. The Principles of Microbe and Cell Cultivation 1975 Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  20. Rho Y.T., Jeong B.C., Lee K.J. Molecular cloning of alkaline protease in Streptomyces spp role of alkaline protease in the cell differentiation and optimization of fermentation. In Proceedings of the 3rd Conference on Molecular biology & Genetic Engineering 1988 Seoul: Korean Society of Molecular Biology; pp 57–62
    [Google Scholar]
  21. Rho Y.T., Kim J.W., Lee K.J. Regulation of alkaline protease biosynthesis in Streptomyces spp.: effects of culture environments on gene expression. In Proceedings of the 4th Conference on Molecular biology & Genetic Engineering 1989 Seoul: Korean Society of Molecular Biology; pp 91–94
    [Google Scholar]
  22. Rho Y.T., Kim H.T., Oh K.H., Kang H.I., Ward A.C., Goodfellow M., Hah Y.C., Lee K.J. Numerical identification of a Streptomyces strain producing spores in submerged culture. Korean J Microbiol 1992; 30:278–285
    [Google Scholar]
  23. Shin H.S., Lee K.J. Regulation of extracellular alkaline protease biosynthesis in a strain of Streptomyces spp. Korean J Microbiol 1986; 24:32–37
    [Google Scholar]
  24. Tan H., Chater K.F. Two developmentally controlled promoters of Streptomyces coelicolor A3(2) that resemble the major class of mobility-related promoters in other bacteria. J bacteriol 1993; 175:933–940
    [Google Scholar]
  25. Wellington E.M.H., Williams S.T. Preservation of actinomycete inoculum in frozen glycerol. Microbios Eett 1978; 6:151–157
    [Google Scholar]
  26. Williams S.T., Goodfellow M., Alderson G., Wellington E.M.H., Sneath P.H.A., Sackin M. Numerical classification of Streptomyces and related genera. J Gen Microbiol 1983a; 129:1743–1813
    [Google Scholar]
  27. Williams S.T., Goodfellow M., Wellington E.M.H., Vickers J.C., Alderson G., Sneath P.H.A., Sackin M., Mortimer A.M. A probability matrix for the identification of some streptomycetes. J Gen Microbiol 1983b; 129:1815–1830
    [Google Scholar]
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