Cytokine gene expression was determined in vivo in the lungs and spleens of Mycoplasma pneumoniae-infected BALB/c mice by means of qualitative and semiquantitative PCR-mediated mRNA amplification. During the acute phase of both primary and secondary infections, cytokines commonly associated with innate resistance, TNFα, IFNγ, IL-1β and IL-6, were expressed. In contrast, early expression of the genes for IL-2 and IL-2 receptor was detected only during reinfection. Expression was greater in the lungs than in the spleen, attesting to the rapid accumulation of lymphocytes at the infected site. Interestingly, IL-2 mRNA expression declined rapidly and was no longer detectable after 24 h, whereas IL-10 mRNA levels rose sharply during the same period. During reinfection, mRNAs for TNFα and IL-6 were 10-fold and for IFNγ about 50-fold higher than during primary challenge. The results suggest that the pathogenesis of M. pneumoniae diseases may be associated with elevated expression of proinflammatory cytokines.
CimolaiN., TaylorG.P., MahD., MorrisonB.J. Definition and application of a histopathological scoring scheme for an animal model of acute Mycoplasma pneumoniae pulmonary infection. Microbiol Immunol1992; 36:465–478
EhlersS., MielkeM.E.A., BlankensteinT., HahnH. Kinetic analysis of cytokine gene expression in the liver of naive and immune mice infected with Eisteria monocytogenes. The immediate early phase in innate resistance and acquired immunity. J Immunol1992; 149:3016–3022
FiorentinoD.F., ZlotnikA., VieiraP., MosmannT.R., HowardM., MooreK.W., O'GarraA. IL-10 acts on the antigen-presenting cells to inhibit cytokine production by Thl cells. J Immunol1991; 146:3444–3451
JacobsE., DrewsM., StuhlertA., BiittnerC., KleinP.J., KistM., BredtW. Immunological reactions of guinea pigs following intranasal Mycoplasma pneumoniae infection and immunization with the 168 kDa adherence protein. J Gen Microbiol1988; 134:473–479
KennyG.E., KaiserG.G., CooneyM.K., FoyH.M. Diagnosis of Mycoplasma pneumoniae pneumonia: sensitivities and specificities of serology with lipid antigen and isolation of the organism on soy peptone medium for identification of infections. J Clin Microbiol1990; 28:2087–2093
KindierV., SappinoA.P., GrauG.E., PiguetP.F., VassalliP. The inducing role of tumor necrosis factor in the development of bacterial granulomas during BCG infection. Cell1989; 56:731–740
KitaM., OhmotoY., HiraiY., YamaguchiN., ImanishiJ. Induction of cytokines in human peripheral blood mononuclear cells by mycoplasmas. Microbiol Immunol1992; 5:507–516
McGeeZ.A., Taylor-RobinsonD. Mycoplasmas in medical microbiology and infectious diseases. In Medical Microbiology and Infectious Diseases1981 Edited by BraudeA.I. Philadelphia: W.B. Saunders; pp 522–528
MielkeM.E.A., RosenH., BrockeS., PetersC., HahnH. Protective immunity and granuloma formation are mediated by two distinct tumor necrosis factor alpha-and gamma-interferon dependent T cell-phagocyte interactions in murine listeriosis: dissociation on the basis of phagocyte adhesion mechanisms. Infect lmmun1992; 60:1875–1882
PietschK., JacobsE. Characterization of the cellular response of spleen cells in BALB/c mice inoculated with Mycoplasma pneumoniae or the PI protein. Med Microbiol Immunol1993; 182:77–85
PirmezC., YamamuraM., UyemuraK., Paes-OliveiraM., Conceicao-SilvaF. Cytokine patterns in the pathogenesis of human leishmaniasis. J Clin Invest1993; 91:1390–1395