1887

Abstract

Cytokine gene expression was determined in the lungs and spleens of -infected BALB/c mice by means of qualitative and semiquantitative PCR-mediated mRNA amplification. During the acute phase of both primary and secondary infections, cytokines commonly associated with innate resistance, TNFα, IFNγ, IL-1β and IL-6, were expressed. In contrast, early expression of the genes for IL-2 and IL-2 receptor was detected only during reinfection. Expression was greater in the lungs than in the spleen, attesting to the rapid accumulation of lymphocytes at the infected site. Interestingly, IL-2 mRNA expression declined rapidly and was no longer detectable after 24 h, whereas IL-10 mRNA levels rose sharply during the same period. During reinfection, mRNAs for TNFα and IL-6 were 10-fold and for IFNγ about 50-fold higher than during primary challenge. The results suggest that the pathogenesis of diseases may be associated with elevated expression of proinflammatory cytokines.

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1994-08-01
2021-10-16
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References

  1. Barile M.F. Immunization against Mycoplasma pneumoniae disease: a review. Isr J Med Sci 1984; 20:912–915
    [Google Scholar]
  2. Barile M.F., Chandler D.K.F., Yoshida H., Grabowski M.W., Harasawa R., Razin S. Parameters of Mycoplasma pneumoniae infection in Syrian hamsters. Infect Immun 1988; 56:2443–2449
    [Google Scholar]
  3. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 162:156–159
    [Google Scholar]
  4. Cimolai N., Taylor G.P., Mah D., Morrison B.J. Definition and application of a histopathological scoring scheme for an animal model of acute Mycoplasma pneumoniae pulmonary infection. Microbiol Immunol 1992; 36:465–478
    [Google Scholar]
  5. Cuturi M.C., Anegon I., Sherman F., Loudon R., Clark S.C., Perussia B., Trinchieri G. Production of hematopoietic colony-stimulating factors by human natural killer cells. J Exp Med 1989; 169:569–583
    [Google Scholar]
  6. Ehlers S., Smith K.A. Differentiation of T cell lympho-kine gene expression: the in vitro acquisition of T cell memory. J Exp Med 1991; 173:25–36
    [Google Scholar]
  7. Ehlers S., Mielke M.E.A., Blankenstein T., Hahn H. Kinetic analysis of cytokine gene expression in the liver of naive and immune mice infected with Eisteria monocytogenes. The immediate early phase in innate resistance and acquired immunity. J Immunol 1992; 149:3016–3022
    [Google Scholar]
  8. Fiorentino D.F., Zlotnik A., Vieira P., Mosmann T.R., Howard M., Moore K.W., O'Garra A. IL-10 acts on the antigen-presenting cells to inhibit cytokine production by Thl cells. J Immunol 1991; 146:3444–3451
    [Google Scholar]
  9. Hayflick L. Cell cultures and mycoplasmas. Tex Rep Biol Med 1965; 23:285–303
    [Google Scholar]
  10. Hirano T., Akira S., Taga T., Kishimoto T. Biological and clinical aspects of interleukin 6. Immunol Today 1990; 11:443–449
    [Google Scholar]
  11. Jacobs E., Drews M., Stuhlert A., Biittner C., Klein P.J., Kist M., Bredt W. Immunological reactions of guinea pigs following intranasal Mycoplasma pneumoniae infection and immunization with the 168 kDa adherence protein. J Gen Microbiol 1988; 134:473–479
    [Google Scholar]
  12. Jacobs E., Rock R., Dalehite L., Bredt W. A B-cell T-cell linked epitope of Mycoplasma pneumoniae. Infect lmmun 1990; 58:2464–2469
    [Google Scholar]
  13. Jacobs E. Mycoplasma pneumoniae virulence factors and the immune response. Rep Med Microbiol 1991; 2:83–90
    [Google Scholar]
  14. Kenny G.E., Kaiser G.G., Cooney M.K., Foy H.M. Diagnosis of Mycoplasma pneumoniae pneumonia: sensitivities and specificities of serology with lipid antigen and isolation of the organism on soy peptone medium for identification of infections. J Clin Microbiol 1990; 28:2087–2093
    [Google Scholar]
  15. Kindier V., Sappino A.P., Grau G.E., Piguet P.F., Vassalli P. The inducing role of tumor necrosis factor in the development of bacterial granulomas during BCG infection. Cell 1989; 56:731–740
    [Google Scholar]
  16. Kist M., Jacobs E., Bredt W. Release of Mycoplasma pneumoniae substances after phagocytosis by guinea pig alveolar macrophages. Infect lmmun 1982; 36:357–362
    [Google Scholar]
  17. Kita M., Ohmoto Y., Hirai Y., Yamaguchi N., Imanishi J. Induction of cytokines in human peripheral blood mononuclear cells by mycoplasmas. Microbiol Immunol 1992; 5:507–516
    [Google Scholar]
  18. Lind K., Hoier-Madson M., Wilk A., Clyde W.A. Jr Antibodies to the mitotic spindle apparatus in patients with Mycoplasma pneumoniae infection. Immunol & Infect Dis 1992; 2:249–255
    [Google Scholar]
  19. McGee Z.A., Taylor-Robinson D. Mycoplasmas in medical microbiology and infectious diseases. In Medical Microbiology and Infectious Diseases 1981 Edited by Braude A.I. Philadelphia: W.B. Saunders; pp 522–528
    [Google Scholar]
  20. Mielke M.E.A., Rosen H., Brocke S., Peters C., Hahn H. Protective immunity and granuloma formation are mediated by two distinct tumor necrosis factor alpha-and gamma-interferon dependent T cell-phagocyte interactions in murine listeriosis: dissociation on the basis of phagocyte adhesion mechanisms. Infect lmmun 1992; 60:1875–1882
    [Google Scholar]
  21. Murray L.J., Lee R., Martens C. In vivo cytokine gene expression in T cell subsets of the autoimmune MRL/Mp-Ipr/Ipr mouse. Eur J Immunol 1990; 20:163–170
    [Google Scholar]
  22. Nakayama T., Sonoda S., Urano T., Osano M., Maehara N., Sasaki K., Hayatsu E., Makino S. Interferon production during the course of Mycoplasma pneumoniae infection. Pediatr Infect Dis J 1992; 11:72–77
    [Google Scholar]
  23. O'Garra A., Vieira P. Polymerase chain reaction for detection of cytokine gene expression. Curr Opin Immunol 1992; 4:211–215
    [Google Scholar]
  24. Pabst R. Is BALT a major component of the human lung immune system. Immunol Today 1992; 13:119–122
    [Google Scholar]
  25. Pietsch K., Jacobs E. Characterization of the cellular response of spleen cells in BALB/c mice inoculated with Mycoplasma pneumoniae or the PI protein. Med Microbiol Immunol 1993; 182:77–85
    [Google Scholar]
  26. Pirmez C., Yamamura M., Uyemura K., Paes-Oliveira M., Conceicao-Silva F. Cytokine patterns in the pathogenesis of human leishmaniasis. J Clin Invest 1993; 91:1390–1395
    [Google Scholar]
  27. Platzer C., Richter G., Ueberla K., Mueller W., Bloecker H., Diamantenstein T. Analysis of cytokine mRNA levels in interleukin-4-transgenic mice by quantitative polymerase chain reaction. Eur J Immunol 1992; 22:1179–1184
    [Google Scholar]
  28. Razin S., Jacobs E. Review article: Mycoplasma adhesion. J Gen Microbiol 1992; 138:407–422
    [Google Scholar]
  29. Rollins S., Colby T., Clayton F. Open lung biopsy in Mycoplasma pneumoniae pneumonia. Arch Pathol Lab Med 1986; 110:34–41
    [Google Scholar]
  30. Street N.E., Mosmann T.R. Functional diversity of T lymphocytes due to secretion of different cytokine patterns. LASER J 1991; 5:171–177
    [Google Scholar]
  31. Tsunekawa H., Takagi E., Kishimoto H., Shimokata K. Depressed cellular immunity in Mycoplasma pneumoniae pneumonia. Eur J Respir Dis 1987; 70:293–299
    [Google Scholar]
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