@article{mbs:/content/journal/micro/10.1099/13500872-140-8-1989, author = "Ariza, Maria A. and Martín-Luengo, Francisco and Valero-Guillén, Pedro L.", title = "A family of diacyltrehaloses isolated from Mycobacterium fortuitum", journal= "Microbiology", year = "1994", volume = "140", number = "8", pages = "1989-1994", doi = "https://doi.org/10.1099/13500872-140-8-1989", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-140-8-1989", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "Mycobacterium fortuitum", keywords = "acyltrehalose", keywords = "glycolipid", abstract = "A trehalose-containing glycolipid was detected in several strains of Mycobacterium fortuitum and characterized as 2,3-di-O-acyltrehalose (DAT) by combined NMR spectroscopy, IR spectroscopy, GLC and GLC-MS. Lipid constituents of the molecule were identified as a mixture of straight-chain (14-18 carbon atoms) and methyl-branched-chain (17-21 carbon atoms) fatty acyl groups. DAT was further fractionated by reverse phase TLC into four fractions that were designated DAT-I-DAT-IV. DAT-I contained 70-75% straight-chain acyl substituents (hexadecanoyl and octadecanoyl predominating) and 25-30% 2-methyl branched substituents (mainly 2-methyl octadecadienoyl). DAT-II was composed of a mixture in which the acyl groups were almost exclusively 2-methyl branched, with 2-methyl octadecadienoyl and 2-methyl octadecen-2-oyl predominating. DAT-III, which was the major isolated fraction, consisted of compounds in which the ratio linear to branched acyl groups varied between 0.8 to 0.9, 2-methyl octadecen-2-oyl, hexadecanoyl and octadecanoyl being the most abundant. Finally, DAT-IV comprised a mixture of DAT molecules containing mostly 2-methyl octadecadienoyl, 2-methyl octadecen-2-oyl, 2-methyl eicosadienoyl and 2-methyl eicosen-2-oyl groups.", }